Seminal Attributes and Semen Cryo-banking of Nepalese Indigenous Achhami (Bos indicus) Bull under Ex-situ Conservation

Morphometric features, seminal profile and diluters effect on post-thaw semen quality of Munshiganj cattle in Bangladesh

“…Previous investigators [ 48 ] reported that the average total motility was 67.98 ± 7.08 %, progressive motility 43.28 ± 4.94 %, static motility 32.02 ± 7.08 %, bent tail 5.26 ± 0.87 %, coiled tail 0.56 ± 0.11 %, DMR 1.75 ± 0.25 %, distal droplet 1.32 ± 0.23 %, proximal droplet 54.16 ± 4.15 % in post thawed semen which corroborates with the findings of the present study. In Achhami bull, post thaw progressive motility and slow motility were 75 % and 7.4 %, respectively [ 81 ] whereas in Gir bull the post thaw progressive motility was 53.81 ± 0.61 % [ 82 ]. The post thaw coiled tail were 2.3 ± 0.4 % in local and 2.2 ± 0.3 % in crossbred bull found in Bangladesh [ 83 ].…”

“…Progressive motility of frozen thawed semen diluted with Tris egg yolk was 68.19 ± 0.46 % in Holstein Friesian bull and 56.54 ± 0.25 % in Sahiwal bull [ 88 ]. On the other hand, in a study in Achhami bull in Nepal, it was found that post-thaw progressive motility 75.00 % in egg yolk-tris citrate based extender [ 81 ].…”

“…Previous researchers [ 88 ] studied Tris egg yolk effect on frozen semen and found that slow motility was 9.21 ± 0.46 % in Holstein Friesian bull and 11.11 ± 0.55 % in Sahiwal bull. In a study with Achhami bull semen in Nepal, it was found that slow motility was 7.4 % in post-thaw semen diluted with egg yolk tris-citrate based extender [ 81 ].…”

Supplementation of cryoprotective extender with resveratrol decreases apoptosis index and reactive oxygen species levels in post-thaw dog sperm

“…The use of artificial insemination (AI) has expanded widely in livestock and canine reproduction. This technique reduces the use of breeding dogs, which requires transportation of animals, is relatively costly, is time-consuming, and poses risks of disease transmission [1][2][3]. AI also facilitates breeding of the required, usually genetically superior dogs, both within and among countries [4,5].…”