Seminal Cytology and Reproductive Toxicology of N-Chloropiperidine

Abstract

Induction of sperm abnormality in mice and hamster and reproductive toxicity in mice was examined following chronic exposures (ad libitum) to N-chloropiperidine (NCP). The results showed that significant levels of abnormal sperm in the NCP treated group were observed after five weeks of exposure. The time of the appearance of NCP-induced anomalous sperm suggested that the compound did not affect late spermatocytes and spermiogenesis. Reproductive toxicological analysis based on moles, implantations, and offspring per pregnancy revealed that while the toxic effect of ethylmethane sulfonate was evident within the first week of treatment, NCP-induced toxicity was observed only after five weeks of treatment. The data further showed that in matings where both parents were exposed to NCP, the frequencies of moles, implantations, and offspring per pregnancy were fewer than those observed in single parent exposure.

Scientific Opinion on Flavouring Group Evaluation 86, Revision 2 (FGE.86Rev2): Consideration of aliphatic and arylalkyl amines and amides evaluated by JECFA (65th meeting)

“…In another study, an oral dose of 400 mg/kg bw/day of piperidine for 40-100 days did not induce sperm shape abnormalities in mice or hamsters (Bempong and Scully, 1983).…”

“…Mice given piperine at doses of up to 75 mg/kg bw/day by gavage or up to 4 mg/kg bw per day by intraperitoneal injection for 5 days showed no sperm shape abnormalities or dominant lethal mutations (Muralidhara and Narasimhamurthy, 1990;Karekar et al, 1996;Daware et al, 2000). In another study, an oral dose of 400 mg/kg bw/day of piperidine for 40-100 days did not induce sperm shape abnormalities in mice or hamsters (Bempong and Scully, 1983).…”

Scientific Opinion on Flavouring Group Evaluation 86, Revision 1 (FGE.86Rev1): Consideration of aliphatic and aromatic amines and amides evaluated by JECFA (65th meeting)

“…Mice given piperine at doses of up to 75 mg/kg bw/day by gavage or up to 4 mg/kg bw per day by intraperitoneal injection for 5 days showed no sperm shape abnormalities or dominant lethal mutations (Muralidhara & Narasimhamurthy, 1990;Karekar et al, 1996;Daware et al, 2000). In another study, an oral dose of 400 mg/kg bw/day of piperidine for 40-100 days did not induce sperm shape abnormalities in mice or hamsters (Bempong & Scully, 1983).…”

“…In another study, an oral dose of 400 mg/kg bw/day of piperidine for 40-100 days did not induce sperm shape abnormalities in mice or hamsters (Bempong & Scully, 1983).…”

Flavouring Group Evaluation 86, (FGE.86) - Consideration of aliphatic and aromatic amines and amides evaluated by JECFA (65th meeting) - Scientific Opinion of the Panel on Food Additives - Flavourings, Processing Aids and Materials in Contact with Food

“…Mice given piperine at doses of up to 75 mg/kg bw/day by gavage or up to 4 mg/kg bw/day by intraperitoneal injection for 5 days showed no sperm shape abnormalities or dominant lethal mutations (Muralidhara & Narasimhamurthy, 1990;Karekar et al, 1996;Daware et al, 2000). In another study, an oral dose of 400 mg/kg bw/day of piperidine for 40-100 days did not induce sperm shape abnormalities in mice or hamsters (Bempong & Scully, 1983). Negative results were reported in bacterial assays for reverse mutation with 15 aliphatic and aromatic amine and amide derivatives: ethylamine, isopropylamine, butylamine, isobutylamine, sec-butylamine, pentylamine, acetamide, 2-isopropyl-N,2,3-trimethylbutyramide, N-isobutyl (E,E)-2,4-decadienamide, piperine, piperidine, pyrrolidine, trimethylamine, triethylamine and piperazine.…”