Background: Infertility is a condition with psychological, economic, medical implications resulting in trauma, stress, particularly in a social set-up, with a strong emphasis on child-bearing. The prevalence of infertility in the general population is 8-12% worldwide. Of this, the male factor is responsible for 40-50%.Semen analysis is an indispensable diagnostic tool in the evaluation of the male partners of infertile couples. Materials and Method: Seminal fluid from the male partners was analysed in the clinical laboratory, JIIU'S IIMSR Medical College, Warudi, Jalna during June 2018 to June 2019 using the World Health Organization (WHO) 2010 criteria for human semen characteristics. Parameters like semen volume, liquefaction time, sperm concentration, motility, vitality, morphology were evaluated. Results: Total 122 males were evaluated in the study. Mean age of presentation was 28.95 yrs. 76 cases [62.30%] found to have abnormal semen analysis and 46 [37.70%] with normal pattern. Abnormalities in sperm no. were seen as most common abnormality 40 patients [32.79%], followed by abnormality in motility 20 patients [16.39%]. Normozoospermia [48.36%], Asthenozoospermia [14.75%], Teratozoospermia [2.46%], Asthenozoospermia with teratozoospermia [1.64%], Oligozoospermia [9.02%], Oligoasthenozoospermia [6.56%], Oligozoospermia with teratozoospermia[3.28%], Oligoastheno-teratozoospermia [2.46%], Azoospermia [11.47%] Conclusion: Though semen analysis has limited role in evaluation of male factor infertility but still it is indispensable and cost effective tool in evaluation of male factor infertility, particularly in rural population.