Seminal fluid proteins differ in abundance between genetic lineages of honeybees

Baer, Boris; Zareie, Reza; Paynter, Ellen; Poland, Veronica; Millar, A. Harvey

doi:10.1016/j.jprot.2012.08.002

Cited by 38 publications

(55 citation statements)

References 46 publications

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“…As would be predicted, SFPs identified in this study possessed a 520 significantly higher proportion of predicted secretion signals than sperm proteins and 521 were, on average, highly specific or biased towards expression in the MAG. Additionally, 522 analysis of the functional composition of our proteomes revealed that they were closely 523 aligned with the results of previous sperm [32][33][34]63] and SFP studies in insects [30,57]. 524 For example, our expanded sperm proteome was highly enriched for proteins related to 525 flagellar structure, including microtubules, dynein complexes, and ciliar components, 526 and proteins likely associated with the mitochondrial derivatives, which are a 527 predominant structure in mosquito sperm [68,69] and that of other insects.…”

supporting

confidence: 68%

Reproductive functions and genetic architecture of the seminal fluid and sperm proteomes of the mosquitoAedes aegypti

Degner

Ahmed-Braimah

Borziak

et al. 2018

Preprint

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The yellow fever mosquito, Aedes aegypti, transmits several viruses, including dengue, Zika, and chikungunya. Some proposed efforts to control this vector involve manipulating reproduction to suppress wild populations or replacing them with disease-resistant mosquitoes. The design of such strategies requires an intimate knowledge of reproductive processes, yet our basic understanding of reproductive genetics in this vector remains largely incomplete. To accelerate future investigations, we have comprehensively catalogued sperm and seminal fluid proteins (SFPs) transferred to females in the ejaculate using tandem mass spectrometry. By excluding female-derived proteins using an isotopic labelling approach, we identified 870 sperm proteins and 280 seminal fluid proteins. Functional composition analysis revealed parallels with known aspects of sperm biology and SFP function in other insects. To corroborate our proteome characterization, we also generated transcriptomes for testes and the male accessory glands-the primary contributors to Ae. aegypti sperm and seminal fluid, respectively. Differential gene expression of accessory glands from virgin and mated males suggests that protein translation is upregulated post-mating. Several SFP transcripts were also modulated after mating, but >90% remained unchanged. Finally, a significant enrichment of SFPs was observed on chromosome 1, which harbors the male sex determining locus in this species. Our study provides a comprehensive proteomic and transcriptomic characterization of ejaculate production and composition and thus provides a foundation for future investigations of Ae. aegypti reproductive biology, from functional analysis of individual proteins to broader examination of reproductive processes.

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supporting

confidence: 68%

Reproductive functions and genetic architecture of the seminal fluid and sperm proteomes of the mosquitoAedes aegypti

Degner

Ahmed-Braimah

Borziak

et al. 2018

Preprint

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“…These authors found a negative correlation between senescence and sperm viability, while a significant interaction between age and colony was also observed (a decline in sperm viability was observed in three of the five colonies). The potential mechanism of differences in sperm viability among colonies may be related to differences in seminal fluid protein abundance (Baer et al 2012). Perhaps such differences reflect the mechanism of IMD effects on the reproductive potential of drones.…”

Section: Discussionmentioning

confidence: 99%

Sperm parameters of honeybee drones exposed to imidacloprid

et al. 2016

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-The objective of this study was to evaluate the effects of chronical exposure of honeybee drones to environmental (5 ppb) and non-environmental concentration (200 ppb) of imidacloprid (IMD) on sperm concentration, motility, viability, and mitochondrial membrane potential measured in semen obtained from 180 drones originating from 18 colonies. The results demonstrate that IMD exposure did not affect sperm concentration; however, there were significant differences in concentration within colonies. IMD exposure was associated with reductions in sperm motility, which also varied within colonies. Statistically significant interactions between IMD exposure and colony were found for active mitochondria and sperm viability. Our results strongly suggest that neonicotinoids can negatively affect honeybee drone sperm quality. It is important to emphasize that IMD actions can be strongly modulated according to the colony.Apis mellifera / imidacloprid / spermatozoa / motility / viability

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“…Numbers refer to species studied and references, as follows: (1) S podoptera litura (Zhang et al., ); C hilo suppressalis (Xia et al., ); A grotis ipsilon (Gu et al., ); A grotis segetum (Strandh et al., ); S esamia inferens (Zhang et al., ); B ombyx mori (Dani et al., ); (2) A pis mellifera (Iovinella et al., ); (3) L eptopilina heterotoma (Heavner et al., ); P teromalus puparum (Wang et al., ); S irex noctilio (Wang et al., ); A pis mellifera (Li et al., ) (4) D rosophila melanogaster (Dyanov & Dzitoeva, ; Takemori & Yamamoto, ); (5) H elicoverpa armigera (Y.L. Sun et al., 2012 b ); (6) L ocusta migratoria (Ban et al., ; Zhou et al., ); (7) A edes aegypti (Li et al., ; Sirot et al., ); (8) A pis mellifera (Baer et al., ); (9) T ribolium castaneum (Xu et al., ); (10) P eriplaneta americana (Nomura et al., ; Kitabayashi et al., ); (11) A pis mellifera (Maleszka et al., ); (12) S olenopsis invicta (Cheng et al., ); (13) L ocusta migratoria (Guo et al., ); (14) A edes aegypti (Calvo et al., ); A nopheles stephensi (Isawa et al., ); (15) P hormia regina (Ishida et al., ); (16) M amestra brassicae (Nagnan‐Le Meillour et al., ); H elicoverpa armigera (Y.L. Liu et al., 2014 b ; Zhu et al., 2016 a ); (17) H elicoverpa armigera and other species (Zhu et al., 2016 a ); (18) B ombyx mori (Xuan et al., ); (19) B emisia tabaci (G.X.…”

Section: Multiple Functions Of Obps and Cspsmentioning

confidence: 99%

“…Other examples of OBPs produced in the sperm and transferred to females during mating include OBP22 of the mosquito A. aegypti (Li et al, 2008;Sirot et al, 2008), CSP3 and OBP9 of A. mellifera in the seminal fluid (Baer et al, 2012), two OBPs of Tribolium castaneum (Xu, Baulding & Palli, 2013) and OBP9 of the moth H. armigera (Y.L. Sun et al, 2012b).…”

Section: (2) Pheromone Glands: Releasing Semiochemicalsmentioning

confidence: 99%

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

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Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non-sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol-binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti-inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment.

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Seminal fluid proteins differ in abundance between genetic lineages of honeybees

Cited by 38 publications

References 46 publications

Reproductive functions and genetic architecture of the seminal fluid and sperm proteomes of the mosquitoAedes aegypti

Reproductive functions and genetic architecture of the seminal fluid and sperm proteomes of the mosquitoAedes aegypti

Sperm parameters of honeybee drones exposed to imidacloprid

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

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