Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility

Abstract

Objectives: Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels with sperm parameters.Patients and methods: Specimens were divided into two groups: group 1. normospermia (n=20); group 2. oligoasthenospermia (n=31). Seminal MDA concentration was measured by thiobarbituric acid reaction method. Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide. Seminal MDA levels and GPx activities in both groups were compared.Results: MDA concentrations in both groups were significantly different (1.52 ± 0.75 vs. 2.25 ± 0.88 nM, p = 0.0021). GPx activities in both groups were non-significantly different (0.48 ± 0.11 vs. 0.47 ± 0.12 U/ml). MDA levels were negatively correlated with the sperm motility (MDA = -0.014 x motility + 2.62, p =0.017) and concentration (MDA = -0.0045 x concentration + 2.23, p = 0.0166). GPx activities were positively but non-significantly correlated with the sperm concentration and sperm motility.Conclusions: Seminal MDA concentrations are negatively correlated with sperm concentration and motility, which might provide a simple and useful tool in predicting sperm parameters. GPx activity is non-significantly correlated with the seminal quality. Roles of seminal MDA upon spermatogenesis merits further surveys.

Relationship between seminal malondialdehyde levels and sperm quality in fertile and infertile men

“…Suleiman et al (1996) demonstrated that the MDA concentration in the seminal plasma was not correlated with the sperm concentration and motility. In this work the negative significant correlation was observed between lipid peroxidation with sperm concentration, motility and normal morphology between fertile and infertile men which was compatible with the findings of Kobayashi et al (1991), Huang et al (2000), Hsieh et al (2006), Zalata et al (2004), Suleiman et al (1996) and Gomez et al (1998). But this correlation was not significant in the fertile or infertile men.…”

“…Indeed, this decrease in fluidity could affect the membrane transport activity and thereby affect on the surviving of sperm. A number of studies have shown that lipid peroxidation affects the sperm concentration, motility, morphology and related with poor sperm quality (Huang et al 2000;Hsieh et al, 2006;Gomez et al, 1998). Kobayashi et al (1991) demonstrated that MDA level in spermatozoa was significantly related to the number of immotile sperm.…”

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

“…Sperm membrane lipid peroxidation status, measured by malondialdehyde (MDA) concentration, is the main nonconventional measurable marker of oxidative stress in semen. Malondialdehyde levels have often been correlated with deterioration of sperm quality [82][83][84]. An association between the presence of some known pathogens, such as U. urealyticum or C. trachomatis, and the induction of sperm lipid peroxidation, as judged by the MDA levels, has also been suggested [17,26].…”

Influence of oxidative stress on functional integrity of human spermatozoal membrane

“…Samples showing more than 60% of swollen spermatozoa were classified as HOS positive and those with less than 60% as HOS negative 6 . Estimation of seminal plasma malondialdehyde (MDA) concentration: Seminal MDA concentration was measured by thiobarbituric acid reaction method 7 . …”