Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts

Chen, Joseph C.; Johnson, Brittni; Erikson, David W.; Piltonen, Terhi; Barragan, Fatima; Chu, Simon; Kohgadai, Nargis; Irwin, Juan C.; Greene, Warner C.; Giudice, Linda C.; Roan, Nadia R.

doi:10.1093/humrep/deu047

Cited by 65 publications

(103 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Growing understanding of how seminal fluid influences the periconception cytokine environment in mice indicates that clinical studies are warranted to determine its impact on oviduct and endometrial cytokines in women. Notwithstanding the different reproductive tract anatomy and reduced access of seminal fluid to the higher tract compared with mouse, there is evidence that sperm absorb seminal fluid signalling molecules including TGFβ (Chu et al 1996) and that this reaches the higher regions of the tract (Bulletti et al 1997), where it can alter endometrial cytokine production (Gutsche et al 2003;Chen et al 2014). …”

Section: Cytokines As Mediators Of Paternal Influence On Embryo Survimentioning

confidence: 99%

Female Tract Cytokines and Developmental Programming in Embryos

Robertson

Chin

Schjenken

et al. 2015

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

In the physiological situation, cytokines are pivotal mediators of communication between the maternal tract and the embryo. Compelling evidence shows that cytokines emanating from the oviduct and uterus confer a sophisticated mechanism for 'fine-tuning' of embryo development, influencing a range of cellular events from cell survival and metabolism, through division and differentiation, and potentially exerting long-term impact through epigenetic remodelling. The balance between survival agents, including GM-CSF, CSF1, LIF, HB-EGF and IGFII, against apoptosis-inducing factors such as TNFα, TRAIL and IFNg, influence the course of preimplantation development, causing embryos to develop normally, adapt to varying maternal environments, or in some cases to arrest and undergo demise. Maternal cytokine-mediated pathways help mediate the biological effects of embryo programming, embryo plasticity and adaptation, and maternal tract quality control. Thus maternal cytokines exert influence not only on fertility and pregnancy progression but on the developmental trajectory and health of offspring. Defining a clear understanding of the biology of cytokine networks influencing the embryo is essential to support optimal outcomes in natural and assisted conception.

show abstract

Section: Cytokines As Mediators Of Paternal Influence On Embryo Survimentioning

confidence: 99%

Female Tract Cytokines and Developmental Programming in Embryos

Robertson

Chin

Schjenken

et al. 2015

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

show abstract

“…Fresh eEC culture is identical to culturing recovered eEC, and as previously described (21, 22). Briefly, freshly isolated epithelial fragments were plated in KSFM with gentamycin (without FBS) on Matrigel-coated plates.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, freshly isolated epithelial fragments were plated in KSFM with gentamycin (without FBS) on Matrigel-coated plates. Also, eSF that were sample-matched to eEC obtained and cultured by methods previously described (21, 22) served as cell type controls for gene expression in situ . The filtered <40-μm single-cell fraction was pelleted and washed with PBS twice to remove residual digestion medium.…”

Section: Methodsmentioning

confidence: 99%

“…Indirect immunofluorescence was conducted as previously reported (21, 22) to identify eEC-specific markers. Briefly, eEC cultured in Matrigel-coated plates (n=5; 2 oocyte donors in ESE, 3 non-oocyte donors in SE) were fixed in ice cold methanol, permeabilized with 0.1% Triton X-100 (Sigma Aldrich), blocked with 10% normal goat serum (Sigma Aldrich), and incubated overnight at 4°C with the following primary antibodies at 1–200 dilution: mouse anti-human KRT18 (1:200; C-7785, Sigma Aldrich), CDH1 (ab1416, Abcam, Cambridge, MA), rabbit anti-human CLDN1 (ab15098, Abcam), rabbit anti-human OCLN (ab31721, Abcam).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA from recovered eEC and eSF was obtained using methods previously reported (21, 22). Briefly, non-polarized eEC cultured on Matrigel-coated plates were harvested and purified using the Nucleospin RNA II Purification Kit (740955-250, Machery Nagel, Bethlehem, PA).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Cryopreservation and recovery of human endometrial epithelial cells with high viability, purity, and functional fidelity

Chen

Hoffman

Arora

et al. 2016

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

Objective To develop a protocol for cryopreservation and recovery of human endometrial epithelial cells (eEC) retaining molecular and functional characteristics of endometrial epithelium in vivo. Design This is an in vitro study using human endometrial cells. Setting University research laboratory. Patients Endometrial biopsies were obtained from premenopausal women undergoing benign gynecological procedures. Interventions Primary eEC were cryopreserved in 1% fetal bovine serum (FBS)/10% dimethyl sulfoxide (DMSO) in Defined Keratinocyte Serum Free Medium (KSFM). Recovered cells were observed for endometrial stromal fibroblast (eSF) contamination and subsequently evaluated for morphology, gene expression, and functional characteristics of freshly cultured eECs and in vivo endometrial epithelium. Main Outcome Measures Analysis of eEC morphology and the absence of eSF contamination; evaluation of epithelial-specific gene and protein expression; assessment of epithelial polarity. Results eEC recovered after cryopreservation (n=5) displayed epithelial morphology and expressed E-cadherin (CDH1), occludin (OCLN), claudin1 (CLDN1), and keratin18 (KRT18). Compared to eSF, recovered eEC displayed increased (P<0.05) expression of epithelial-specific genes AREG, CDH1, DEFB4A, MMP7, and WNT7A, while exhibiting low-to-undetectable (P<0.05) stromal-specific genes COL6A3, HOXA11, MMP2, PDGFRB, and WNT5A. Recovered eEC secrete levels of cytokines and growth factors comparable to freshly cultured eEC. Recovered eEC can formed a polarized monolayer with high transepithelial electrical resistance (TER) and impermeability to small molecules, and expressed apical/basolateral localization of CDH1 and apical localization of OCLN. Conclusion We have developed a protocol for cryopreservation of eEC in which recovered cells after thawing demonstrate morphological, transcriptomic, and functional characteristics of human endometrial epithelium in vivo.

show abstract

Application of seminal plasma to female genital tract prior to embryo transfer in assisted reproductive technology cycles (IVF, ICSI and frozen embryo transfer)

Ata

Abou‐Setta

Seyhan

et al. 2015

Cochrane Database of Systematic Reviews

View full text Add to dashboard Cite

Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts

Abstract: This project was supported by NIH AI083050-04 (W.C.G./L.C.G.); NIH U54HD 055764 (L.C.G.); NIH 1F32HD074423-02 (J.C.C.); DOD W81XWH-11-1-0562 (W.C.G.); NIH 5K12-DK083021-04, NIH 1K99AI104262-01A1, The UCSF Hellman Award (N.R.R.). The authors have nothing to disclose.

Cited by 65 publications

References 80 publications

Female Tract Cytokines and Developmental Programming in Embryos

Female Tract Cytokines and Developmental Programming in Embryos

Cryopreservation and recovery of human endometrial epithelial cells with high viability, purity, and functional fidelity

Application of seminal plasma to female genital tract prior to embryo transfer in assisted reproductive technology cycles (IVF, ICSI and frozen embryo transfer)

Contact Info

Product

Resources

About