Seminal plasma miRNAs in Klinefelter syndrome and in obstructive and non-obstructive azoospermia

Finocchi, Federica; Pelloni, Marianna; Balercia, Giancarlo; Pallotti, Francesco; Radicioni, A.; Lenzi, Andrea; Lombardo, Francesco; Paoli, Donatella

doi:10.1007/s11033-020-05552-x

Cited by 21 publications

(14 citation statements)

References 63 publications

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“…A new investigation on the role of microRNAs found significant underexpression levels of miR‐122‐5p, miR‐34b‐3p, miR‐34c‐5p and miR‐509‐5p in all seminal plasma samples extracted from azoospermic cases. They selected a set of 40 seminal plasma samples of normozoospermic patients as controls, 40 seminal plasma samples of patients having Klinefelter syndrome, 60 seminal plasma samples of non‐obstructive azoospermia patients and 60 seminal plasma samples of obstructive azoospermia patients to perform this retrospective cohort study (Finocchi et al, 2020). The authors also figured out the important role of miR‐122 in causing the aberrant sperm development.…”

Section: Resultsmentioning

confidence: 99%

Using microRNAs as molecular biomarkers for the evaluation of male infertility

Asadpour

Chelan

2021

Andrologia

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Infertility is a multiplex disorder in the reproductive system, and men are responsible for more than half of the cases. Nowadays, semen analysis has been considered the critical assessment test to diagnose infertile men; however, it has limitations so that the cause behind infertility in 40% of infertile men is unrevealed. Weaknesses of semen assessment indicate a global need for novel and better diagnostic tools and biomarkers. MicroRNAs are short (about 18–22 nucleotide length) non‐coding RNAs that control most (>60%) of our protein‐coding genes post‐transcriptionally. These molecules are aberrant in the body fluids, and abnormal alterations in their expression level can signify a specific disease such as infertility. Therefore, microRNAs can be novel candidate biomarkers that can diagnose different types of male infertility, including azoospermia, oligozoospermia, asthenozoospermia and teratozoospermia. This narrative review aimed to collect and sum up new papers published about the significant role of microRNAs in different male infertility categories.

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Section: Resultsmentioning

confidence: 99%

Using microRNAs as molecular biomarkers for the evaluation of male infertility

Asadpour

Chelan

2021

Andrologia

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“…Particularly, three miRNAs are reported by more than two studies: miRNAs belonging to the miR-34, miR-122, and miR-509 families (Table 1). They all are down-regulated in patients with NOA and in those with oligozoospermia compared with fertile controls [26,31,32]. Hence, multi-center studies on ample cohorts should be accomplished to validate their diagnostic value in patients with idiopathic male infertility.…”

Section: Resultsmentioning

confidence: 99%

Seminal Plasma Transcriptome and Proteome: Towards a Molecular Approach in the Diagnosis of Idiopathic Male Infertility

Cannarella

Barbagallo

Crafa

et al. 2020

IJMS

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As the “-omic” technology has largely developed, its application in the field of medical science seems a highly promising tool to clarify the etiology, at least in part, of the so-called idiopathic male infertility. The seminal plasma (SP) is made-up of secretions coming from the male accessory glands, namely epididymis, seminal vesicles, and prostate. It is not only a medium for sperm transport since it is able to modulate the female reproductive environment and immunity, to allow the acquisition of sperm competence, to influence the sperm RNA content, and even embryo development. The aim of this systematic review was to provide an updated and comprehensive description of the main transcripts and proteins reported by transcriptome and proteome studies performed in the human SP of patients with idiopathic infertility, in the attempt of identifying possible candidate molecular targets. We recurrently found that micro RNA (miR)-34, miR-122, and miR-509 are down-regulated in patients with non-obstructive azoospermia (NOA) and oligozoospermia compared with fertile controls. These molecules may represent interesting targets whose predictive role in testicular sperm extraction (TESE) or assisted reproductive techniques (ART) outcome deserves further investigation. Furthermore, according to the available proteomic studies, ECM1, TEX101, lectingalactoside-binding andsoluble 3 binding protein (LGALS3BP) have been reported as accurate predictors of TESE outcome. Interestingly, ECM1 is differently expressed in patients with different ART outcomes. Further prospective, ample-sized studies are needed to validate these molecular targets that will help in the counseling of patients with NOA or undergoing ART.

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“…MiRNAs were quantified using TaqMan MicroRNA Assay hsa-miR-146a-5p, hsa-miR-34a-5p and hsa-miR-23a-5p, with the endogenous control U6 snRNA for the seminal plasma (Thermo Fisher, Milan, Italy) as previously described [17][18][19].…”

Section: Quantitative Rt-pcr Mirnasmentioning

confidence: 99%

Pro-inflammatory cytokines and microRNAs in male infertility

et al. 2021

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Background Male infertility is a problem that affects 10–15% of men of reproductive age. In particular, gametogenesis is a complex process in which inflammation may play a central role through the secretion of cytokines and the expression of microRNAs. We assessed the potential role of proinflammatory cytokines (TNF-α, IL-6 and IL-1α) and microRNAs (miR-146a-5p, miR-34a-5p and miR-23a-3p) in the seminal plasma of infertile men compared to controls, evaluating their correlation with seminal and biochemical parameters. Methods and results Expression of cytokines and microRNAs was analyzed by ELISA and q-PCR. Our data shows that IL-1α was significantly increased in the azoospermic group compared to controls, TNF-α mRNA was more expressed in the oligozoospermic group than controls. There were no significant differences in miRNAs expression among the three groups. The correlations between sperm parameters and inflammatory markers were evaluated, however no significance was highlighted. Conclusions The determination of each inflammatory marker separately in the seminal plasma of subfertile men, despite some significant differences, does not have a diagnostic value in male infertility even if an assay of selective pro-inflammatory cytokines and microRNAs in the semen may improve the diagnosis of male infertility.

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Seminal plasma miRNAs in Klinefelter syndrome and in obstructive and non-obstructive azoospermia

Cited by 21 publications

References 63 publications

Using microRNAs as molecular biomarkers for the evaluation of male infertility

Using microRNAs as molecular biomarkers for the evaluation of male infertility

Seminal Plasma Transcriptome and Proteome: Towards a Molecular Approach in the Diagnosis of Idiopathic Male Infertility

Pro-inflammatory cytokines and microRNAs in male infertility

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