Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men

Marić, Tihana; Bojanac, Ana Katušić; Matijević, Ana; Ceppi, Marcello; Bruzzone, Marco; Evgeni, Evangelini; Petrović, Tea; Wójcik, Iwona; Trbojević‐Akmačić, Irena; Lauc, Gordan; Ježek, Davor; Fučić, Aleksandra

doi:10.3390/life11090989

Cited by 5 publications

(4 citation statements)

References 70 publications

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“…Terminal Lewis x /Lewis y N- glycans in seminal plasma were implicated in the suppression of innate and adaptive immune responses . Due to the variety potential roles in semen function during fertilization, seminal plasma glycoproteins raise widespread concerns in applications as clinical biomarkers. − Therefore, a comprehensive and global exploration of glycosylation in seminal plasma will expand our understanding of reproductive biology in humans.…”

Section: Introductionmentioning

confidence: 99%

Precision Structural Interpretation of Site-Specific N-Glycans in Seminal Plasma

Xin

You

et al. 2022

J. Proteome Res.

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N-Linked glycoproteins are rich in seminal plasma, playing various essential roles in supporting sperm function and the fertilization process. However, the detailed information on these glycoproteins, particularly site-specific glycan structures, is still limited. In this study, a precision site-specific N-glycoproteome map of human seminal plasma was established by employing the site-specific glycoproteomic approach and a recently developed glycan structure interpretation software, StrucGP. A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins. These glycans were comprised of four types of core structures and 13 branch structures. The majority of identified glycoproteins functioned in response to stimulus and immunity. As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways. Comparison of site-specific glycans between seminal plasma and spermatozoa revealed more complicated glycan structures in seminal plasma than in spermatozoa, even on their shared glycoproteins. These present data will be greatly beneficial for the in-depth structural and functional study of glycosylation in the male reproduction system.

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Section: Introductionmentioning

confidence: 99%

Precision Structural Interpretation of Site-Specific N-Glycans in Seminal Plasma

Xin

You

et al. 2022

J. Proteome Res.

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“…Concanavalin A is able to bind biantennary complex glycans, while does not recognize those with three or four antennae. Marić et al [ 142 ] in their SP N-glycome analysis identified a decreased level of biantennary bisialylated glycans (with or without fucose) accompanying increasing sperm chromatin maturity. This may be read as relatively increased glycan branching accompanying the impaired semen pattern.…”

Section: Seminal Plasma Glycomementioning

confidence: 99%

“…These cells, presenting a panel of immunomodulatory lectins as described in the former paragraphs, enter the microenvironment created by seminal plasma, which makes the direct interaction possible. The second fact that should be emphasized is the presentation of glycoepitopes, which can potentially be ligands for lectins in SP glycoproteins, and also the reports on the changes in their glycosylation pattern, observed in the patients with fertility disorders [ 142 , 144 ], classified as: oligozoospermia—sperm count < 16 mln/mL; asthenozoospermia—<32% of sperm with progress motion; teratozoospermia—<4% sperm with correct morphology; and normozoospermia—the semen fulfilled the WHO recommended values, while male infertility factor was suspected. So far, data on the direct interaction of seminal plasma glycoconjugates with endogenous lectins of the immune system are limited.…”

Section: Seminal Plasma Glycomementioning

confidence: 99%

Seminal Plasma Glycoproteins as Potential Ligands of Lectins Engaged in Immunity Regulation

Olejnik

Ferens-Sieczkowska

2022

IJERPH

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Environmental pollution, chronic stress, and unhealthy lifestyle are factors that negatively affect reproductive potential. Currently, 15–20% of couples in industrialized countries face the problem of infertility. This growing health and social problem prompts researchers to explore the regulatory mechanisms that may be important for successful fertilization. In recent years, more attention has been paid to male infertility factors, including the impact of seminal plasma components on regulation of the female immune response to allogenic sperm, embryo and fetal antigens. Directing this response to the tolerogenic pathway is crucial to achieve a healthy pregnancy. According to the fetoembryonic defense hypothesis, the regulatory mechanism may be associated with the interaction of lectins and immunomodulatory glycoepitopes. Such interactions may involve lectins of dendritic cells and macrophages, recruited to the cervical region immediately after intercourse. Carbohydrate binding receptors include C type lectins, such as DC-SIGN and MGL, as well as galectins and siglecs among others. In this article we discuss the expression of the possible lectin ligands, highly fucosylated and high mannose structures, which may be recognized by DC-SIGN, glycans of varying degrees of sialylation, which may differ in their interaction with siglecs, as well as T and Tn antigens in O-glycans.

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“….jrasb.com motility; improvement of sperm fertilisation characters; reduction of any alteration that can occur in the endocrine and replacement of testicular damages; and reduction of germ cell apoptosis by improving testicular histology [80,81,82]. …”

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confidence: 99%

Role of Antioxidants of Natural Herbs in Management of Male Infertility

Gaznee¹,

Kohli²,

Kumar³

2023

J. Res. Appl. Sci. Biotechnol.

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If you and your spouse have been trying to conceive for a year and neither of you has been successful, there is a possibility that you are one of the 50–80 million people throughout the world who struggle with infertility. There is a male component present in approximately 20%-30% of these instances. It is common knowledge that low-quality sperm and oxidative stress (OS) both have a role in the aetiology of male infertility. Because of the oxidation of DNA, proteins, and lipids, excessive levels of reactive oxygen species (ROS) have the potential to disrupt the viability, motility, and morphology of sperm cells. ROS are responsible for these changes. Methods: Through the use of the PubMed interface, we searched the MEDLINE database for studies that had been completed and published during the past ten years that analysed the effect that antioxidants had on sperm in infertile guys (2012–2022). A number of different phrases, including infertility, alpha-lipoic acid, zinc, folate, coenzyme Q10, selenium, and vitamin, were utilised during the search. The study's findings indicate that inositol serves a purpose in a number of different mechanisms that foster contacts between sperm and oocytes, and that it also affects OS levels in sperm cells by way of its engagement in mitochondrial events. Alpha-lipoic acid (ALA) lessens the damage caused by reactive oxygen species (ROS) and improves the quality of sperm in a number of ways, including motility, morphology, and count. There might be a connection between not getting enough zinc in your diet and having low-quality sperm. Zinc and folate are two nutrients that can boost the quantity and quality of sperm. When taken orally, coenzyme Q10 increases the number of sperm as well as their overall motility and forward movement. A therapy with selenium (Se) results in an improvement in the sperm's overall quality, and this improvement is connected with an increase in ejaculatory production. Only vitamin B12 has been found to improve the quality of sperm; it does this by boosting sperm count and motility and by decreasing sperm DNA damage. Vitamin B12 is the only vitamin that has been proved to do this. Conclusions: Dietary supplementation with antioxidants may improve sperm quality in men with low-quality semen by reducing OS-induced sperm damage and increasing hormone synthesis and spermatozoa concentration, motility, and morphology. This may be achieved in men who also have low levels of antioxidants in their semen. Antioxidants can exert their effects in a variety of ways; hence, it is important for researchers conducting future clinical trials to study the possibility of combining a number of antioxidants.

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Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men

Cited by 5 publications

References 70 publications

Precision Structural Interpretation of Site-Specific N-Glycans in Seminal Plasma

Precision Structural Interpretation of Site-Specific N-Glycans in Seminal Plasma

Seminal Plasma Glycoproteins as Potential Ligands of Lectins Engaged in Immunity Regulation

Role of Antioxidants of Natural Herbs in Management of Male Infertility

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