Seminal plasma protein profiles based on molecular weight from different bull breeds as a potential ovulatory induction factor

Abstract

Seminal plasma is a specific biochemical compound that affects fertility. This study attempts to characterize bull breeds’ seminal plasma (SP) proteins with possible ovulatory induction factor (OIF) activity. The breed samples originated from Friesian Holstein (FH), Belgian Blue Cross (BBx), and Ongole Grade (OG). Centrifug the sperm for 30 minutes at 12,000 rpm. The sample was collected, placed in a microtube, and stored at -20°C. The measurement of protein concentration was done using a Nano-Drop 2000 spectrophotometer. Protein separation was performed using 1D-SDS-PAGE to characterize the protein according to its molecular weight (MW). Protein markers were used to identify the MW proteins, and the acrylamide gels were colored with Coomassie Brilliant Blue. Total protein bands were analyzed with Kruskal-Wallis test, and the identification protein was analyzed descriptively. Proteins of 49-41 kDa and 15-11 kDa were significantly different between breeds (p0.05), while the majority of the MW ranges were not (p>0.05). The proteins with MWs of 22–33 kDa were found to be most commonly in all breeds correlated as potential proteins of OIF. In conclusion, seminal plasma protein was quite varied among the bull breeds. Further research needs to be conducted to obtain the protein from OIF.