Seminal plasma proteins and their relationship with sperm motility and morphology in boars

Lazari, Franciele Lucca de; Sontag, Elistone Rafael; Schneider, Alexander; Moura, Arlindo A.; Vasconcelos, Fábio Perdigão; Nagano, Celso Shiniti; Mattos, Rodrigo Costa; Jobim, Mariana; Bustamante‐Filho, Ivan Cunha

doi:10.1111/and.13222

Cited by 43 publications

(37 citation statements)

References 51 publications

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“…This effect could be explained by BSP (binder sperm proteins) in this fraction, which initiated cholesterol efflux and modulated the conformation of sperm membrane components, leading to a motility increase [ 15 ]. In boars, high concentrations of heparin-non-binding PSP-I protein are associated with reduced total and progressive sperm motility [ 45 ]. A recent study by Suárez et al, 2020 [ 46 ] proved that high concentrations of stallion SP decrease total and progressive motility, as well as some kinematic parameters of spermatozoa post-thaw.…”

Section: Discussionmentioning

confidence: 99%

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Bubeníčková

Maňásková-Postlerová

Šimoník

et al. 2020

IJMS

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Seminal plasma (SP) is the natural environment for spermatozoa and contains a number of components, especially proteins important for successful sperm maturation and fertilization. Nevertheless, in standard frozen stallion insemination doses production, SP is completely removed and is replaced by a semen extender. In the present study, we analyzed the effects of the selected seminal plasma protein groups that might play an important role in reducing the detrimental effects on spermatozoa during the cryopreservation process. SP proteins were separated according to their ability to bind to heparin into heparin-binding (Hep+) and heparin-non-binding (Hep−) fractions. The addition of three concentrations—125, 250, and 500 µg/mL—of each protein fraction was tested. After thawing, the following parameters were assessed: sperm motility (by CASA), plasma membrane integrity (PI staining), and acrosomal membrane integrity (PNA staining) using flow cytometry, and capacitation status (anti-phosphotyrosine antibody) using imaging-based flow cytometry. Our results showed that SP protein fractions had a significant effect on the kinematic parameters of spermatozoa and on a proportion of their subpopulations. The 125 µg/mL of Hep+ protein fraction resulted in increased linearity (LIN) and straightness (STR), moreover, with the highest values of sperm velocities (VAP, VSL), also this group contained the highest proportion of the fast sperm subpopulation. In contrast, the highest percentage of slow subpopulation was in the groups with 500 µg/mL of Hep+ fraction and 250 µg/mL of Hep− fraction. Interestingly, acrosomal membrane integrity was also highest in the groups with Hep+ fraction in concentrations of 125 µg/mL. Our results showed that the addition of protein fractions did not significantly affect the plasma membrane integrity and capacitation status of stallion spermatozoa. Moreover, our results confirmed that the effect of SP proteins on the sperm functionality is concentration-dependent, as has been reported for other species. Our study significantly contributes to the lack of studies dealing with possible use of specific stallion SP fractions in the complex puzzle of the improvement of cryopreservation protocols. It is clear that improvement in this field still needs more outputs from future studies, which should be focused on the effect of individual SP proteins on other sperm functional parameters with further implication on the success of artificial insemination in in vivo conditions.

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Section: Discussionmentioning

confidence: 99%

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Bubeníčková

Maňásková-Postlerová

Šimoník

et al. 2020

IJMS

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“…Its fluid is responsible for maintaining cell function and structural viability; thus, changes in this milieu could result in impaired sperm storage and decreased fertility. In the boar, several studies were published aiming to describe the epididymal proteome (Dacheux et al, 2005; Labas et al, 2015; Syntin et al, 1999, 1996) as well as the seminal plasma protein profile (Gonzalez‐Cadavid et al, 2014; Perez‐Patino et al, 2016, 2019; Strzezek et al, 2005) and molecular markers associated with semen quality (De Lazari et al, 2019) and freezability (Valencia, Gomez, Lopez, Mesa, & Henao, 2017; Vilagran et al, 2015). However, there is a gap in understanding how the epididymal milieu is impacted in pathological conditions, such as HPT‐axis disruption.…”

Section: Discussionmentioning

confidence: 99%

Changes in porcine cauda epididymal fluid proteome by disrupting the HPT axis: Unveiling potential mechanisms of male infertility

Souza

Lopes

Silva

et al. 2020

Molecular Reproduction Devel

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Male infertility or subfertility is frequently associated with disruption of the hypothalamic-pituitary-testis axis events, like secondary hypogonadism. However, little is known how this condition affects the proteomic composition of the epididymal fluid. In the present study, we evaluated the proteomic changes in the cauda epididymal fluid (CEF) in a swine model of secondary hypogonadism induced by anti-GnRH immunization using multidimensional protein identification technology. Seven hundred and eighteen proteins were identified in both GnRH-immunized and control groups. GnRH immunization doubled the number of proteins in the CEF, with 417 proteins being found exclusively in samples from GnRH-immunized boars. CEF from GnRH-immunized boars presented an increase in the number of proteins related to cellular and metabolic processes, with affinity to organic cyclic compounds, small molecules, and heterocyclic compounds, as well changed the enzymatic profile of the CEF. Also, a significant increase in the number of proteins associated to the ubiquitin-proteasome system was identified in CEF from GnRH-immunized animals. These results bring strong evidence of the impact of secondary hypogonadism on the epididymal environment, which is responsible for sperm maturation and storage prior ejaculation. Finally, the differently expressed proteins in the CEF are putative seminal biomarkers for testicular and epididymal disorders caused by secondary hypogonadism.

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“…Although the transfer of proteins to SPZ represents a common feature of seminal exosomes, it is actually discussed whether distinct populations of vesicles carry out different tasks [ 22 , 23 ]. Whatever the case, the analysis of seminal proteome has corroborated the involvement of this fluid in the control of sperm physiology [ 24 ], motility [ 25 ], morphology [ 26 ], concentration [ 27 ] and protection from oxidation [ 28 ]. Interestingly, Ca 2+ signaling machinery required for sperm motility may be directly achieved from SP [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

Candenas

Chianese

2020

IJMS

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Infertility has become a global health issue, with approximately 50% of infertility cases generated by disorders in male reproduction. Spermatozoa are conveyed towards female genital tracts in a safe surrounding provided by the seminal plasma. Interestingly, this dynamically changing medium is a rich source of proteins, essential not only for sperm transport, but also for its protection and maturation. Most of the seminal proteins are acquired by spermatozoa in transit through exosomes (epididymosomes and prostasomes). The high number of seminal proteins, the increasing knowledge of their origins and biological functions and their differential expression in the case of azoospermia, asthenozoospermia, oligozoospermia and teratozoospermia or other conditions of male infertility have allowed the identification of a wide variety of biomarker candidates and their involvement in biological pathways, thus to strongly suggest that the proteomic landscape of seminal plasma may be a potential indicator of sperm dysfunction. This review summarizes the current knowledge in seminal plasma proteomics and its potentiality as a diagnostic tool in different degrees of male infertility.

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Seminal plasma proteins and their relationship with sperm motility and morphology in boars

Cited by 43 publications

References 51 publications

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Changes in porcine cauda epididymal fluid proteome by disrupting the HPT axis: Unveiling potential mechanisms of male infertility

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

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