Seminal Plasma Proteins as Potential Markers of Semen Characteristics in Buffalo and Bovine Bulls

Kadoom, A. K.; El-Keraby, F. E.; Ashour, A. F.; El-Siefy, E. M.

doi:10.21608/jappmu.2016.48715

Cited by 3 publications

(2 citation statements)

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“…Among all protein bands present in seminal plasma and sperm membrane, only four bands having molecular weight 55 kDa (Osteopontin), 30 kDa (Fertility associated antigen -FAA), 26 kDa (Lipocalintype PGD synthase -LPGDs) and 24 kDa (Type-2 tissue inhibitor of metalloproteinases -TIMP-2) were considered as the Fertility Associated Proteins (Fig 1). Similarly, 3 protein bands of molecular weight 14, 15 and 30 kDa were found in bovine bull seminal plasma (Kadoom et al, 2016) and 13 protein bands ranging from 6.5 kDa to 204 kDa in the seminal plasma of Haryana bulls were recorded (Dixit et al, 2016) out of which the presence of 5 protein bands (8.5, 26, 43, 66 and 160 kDa) corroborates with the present findings. The variation in the number of protein bands observed in the present study might be due to the inherent character of species difference between cattle and buffaloes or it may be due to methodology and media involved in protein isolation (Arangasamy et al, 2005).…”

Section: Resultssupporting

confidence: 89%

Qualitative and Quantitative Estimation of Osteopontin in Bull Semen and its Effect on Sperm Quality Parameters and Fertility Rate In vivo

Biswal

Joseph

Kumar

et al. 2022

IJAR

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Background: Osteopontin plays a crucial role in fertilization and subsequent embryonic development. Methods: The fresh semen samples were collected from crossbred Jersey and Haryana breeding bulls. The proteins in the seminal plasma and sperm membrane were precipitated and analysed by SDS-PAGE followed by western blotting. Based on the concentration of osteopontin in the seminal plasma, the bulls were grouped as high (≥20 pg/ml), medium (10-20 pg/ml) and low (≤10 pg/ml) OPN groups. Result: Total 13 and 14 different proteins were identified in the seminal plasma and sperm membrane, respectively. The sperm velocity parameters (straight line velocity and straightness) and functional membrane integrity showed significant difference among high, medium and low OPN groups. Significant difference (P less than 0.05) was observed in malondialdehyde, SOD and glutathione reductase levels. Bulls which are having high osteopontin concentration in the semen had less sperm abnormality, less apoptotic cells, better resistance to cryopreservation, less damaged chromatin and higher conception rate (64.80±1.09) than bulls having low osteopontin concentration in their semen. Therefore, osteopontin may be a valuable protein marker to predict fertility in bulls.

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Section: Resultssupporting

confidence: 89%

Qualitative and Quantitative Estimation of Osteopontin in Bull Semen and its Effect on Sperm Quality Parameters and Fertility Rate In vivo

Biswal

Joseph

Kumar

et al. 2022

IJAR

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“…Both the male and female reproductive characteristics of animals are significantly influenced by the complicated seminal plasma composition (Fuentes-Albero et al, 2021). In many species, seminal plasma proteins have been identified as fertility indicators (Yue et al, 2009;Kadoom et al, 2016). Seminal plasma has proteins and peptides that play a role in the control of the fertilization process, notably through their capacity to bind diverse types of ligands including polysaccharides, lipids, and ions (Mogielnicka-Brzozowska and Kordan, 2011).…”

Section: Introductionmentioning

confidence: 99%

Seminal Plasma Proteins as Biomarkers of Fertility and Semen Traits in Egyptian Barki Rams

Nagar

2022

Annals of Agricultural Science, Moshtohor

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The objective of this study was to identify seminal plasma proteins associated with semen traits (mass motility (MM; %), live sperm (LS; %), abnormal sperm (AS; %), hypo-osmotic swelling test (Host; %), pH, colour, viscosity (Visco), ejaculate volume (VOL; ml), normal chromatin (NC; %), live sperm with reacted acrosome (LR; %), live sperm with unreacted acrosome (LU; %), dead sperm with reacted acrosome (DR; %), dead sperm with unreacted acrosome (DU; %)) in Egyptian Barki rams. Depending on MM%, the animals were divided into three different groups (high (≥ 80% MM), medium (50-80% MM) and low-fertility (≤50% MM) group). The seminal plasma proteins were separated using one-dimensional sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis technique based on their molecular weight. The actual means

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Effects of Cryopreservation of Buffalo and Bovine Spermatozoa on Sperm DNA Damage and Early Embryonic Development

El-Regalaty

2017

Journal of Animal and Poultry Production

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Semen was collected using artificial vagina as per the standard practice from 3 healthy Egyptian buffalo and 3 Holstein Friesian bulls with good body condition, and their age ranged between 4 to 6 yr. Routine semen evaluation was performed. For a better prediction of buffalo and bovine sperm fertility, the first objective was to measure DNA fragmentation in buffalo and bovine bull semen using the alkaline Halo sperm assay. The second objective was to study the influence of cryopreservation process on sperm sustainability of the zygotic and embryonic development. The results revealed that the ejaculate volume and sperm concentration /ml differed (p<0.05) between the bovine and the buffalo bulls. Sperm physical characteristics including sperm motility, livability, percentage of dead sperm, abnormalities, acrosomal damage and values of sperm with halo were found to be different (p<0.05) depending on the species. Semen characteristics of bovine bull are almost better than there of buffalo.Sperm with halo (competent DNA) percentage was. There was a general decrease in post-thawing semen quality parameters including percentages of the live sperm, motility, dead, abnormality, acrosomal damage, and halo sperm value for buffalo and bovine. Moreover, evidence from the current results suggests that the percentage of embryos produced from insemination by fresh sperm had greater (p<0.05) values for the bovine in relation to the buffalo. The rate of development to morula or blastocyst, irrespective of the species or the status of the inseminated sperm (fresh or frozen/thawed) was comparable. It was concluded that (irrespective of the species) sperm freezing/thawing process significantly affected each of sperm physical characteristics and rate of DNA damage. It was also proposed that paternal effects on fertility may go beyond events of fertilization and completion of early stages of embryonic development, and might involve the ability of those embryos to sustain its development to the later stages.

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Seminal Plasma Proteins as Potential Markers of Semen Characteristics in Buffalo and Bovine Bulls

Cited by 3 publications

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Qualitative and Quantitative Estimation of Osteopontin in Bull Semen and its Effect on Sperm Quality Parameters and Fertility Rate In vivo

Qualitative and Quantitative Estimation of Osteopontin in Bull Semen and its Effect on Sperm Quality Parameters and Fertility Rate In vivo

Seminal Plasma Proteins as Biomarkers of Fertility and Semen Traits in Egyptian Barki Rams

Effects of Cryopreservation of Buffalo and Bovine Spermatozoa on Sperm DNA Damage and Early Embryonic Development

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