Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig

Abstract

In pigs, uterine exposure to the constituents of semen is known to increase litter size but the underlying physiological mechanisms remain undefined. Studies in rodents and humans implicate immune modulating moieties in seminal plasma as likely candidates, acting through enhancing the receptivity of the female tract. In this study, the acute and longer term effects of seminal plasma on cytokine expression and leukocyte abundance in the pig endometrium during early pregnancy have been characterised. The reproductive tracts of gonadotrophin-primed pre-pubertal gilts treated with intrauterine infusions of either pooled seminal plasma or phosphate-buffered saline (PBS) were retrieved at 34 h, or on day 5 and day 9 after treatment. Seminal plasma elicited an endometrial inflammatory infiltrate comprised of predominantly macrophages and major histocompatibility complex class II 1 -activated macrophages and dendritic cells. The abundance of these cells was greatest at the pre-ovulatory (34 h) time-point and their increase relative to PBS-treated tissues was maintained until day 9 after seminal plasma treatment. Seminal plasma induced the expression of the cytokines, granulocyte macrophage colony-stimulating factor, interleukin-6 and monocyte chemoattractant protein-1, and the eicosanoid-synthesising enzyme cyclo-oxygenase-2. Expression was maximal 34 h after treatment but altered expression patterns as a consequence of seminal plasma induction persisted through early pregnancy. These changes were accompanied by altered dynamics in pre-implantation embryo development with an increase in the number of embryos and in their viability after seminal plasma treatment. Together, these findings implicate factors in seminal plasma in programming the trajectory of uterine cytokine expression and leukocyte trafficking during early pregnancy and in regulating pre-implantation embryo development in the pig.

Seminal fluid and reproduction: much more than previously thought

“…In addition to neutrophils a large number of antigen presenting cells (APCs) are recruited into the uterine endometrium, including macrophages and dendritic cells (DCs) expressing high levels of MHC class II [60,8]. This response has now been well characterised in other species including rabbit, horse, pig, and human [61][62][63][64]12].…”

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

“…Moreover, some SP-proteins, once into the female genital tract after natural mating or AI, modulate the uterine immune response against the foreign semen, facilitating sperm transport [11], sperm-oocyte fusion [12] and the development of a healthy embryo [13].…”

Semen Promotes the Differentiation of Tolerogenic Dendritic Cells

“…It has been shown that semen deposition in the human female reproductive mucosa results in the induction of a strong inflammatory response that includes the production of inflammatory cytokines and chemokines (7,9,10), the upregulation of cyclooxygenase-2 expression (11), the infiltration of the cervix by neutrophils, and the recruitment of dendritic cells (DCs) (9,12,13). This inflammatory response induced by semen has also been described in mice, pigs, rabbits, and other mammals, and appears to induce the adaptation of the female immune response to promote fertility (14,15).…”