Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections

Meijer, A. E. F. H.; Vloedman, A. H. T.

doi:10.1007/bf00303985

Cited by 45 publications

(4 citation statements)

References 13 publications

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“…Pretreatment procedures -Results,, summarized in Table 4, indicate that a differential portion of native enzymes, present in unfixed tissue processed with the semipermeable membrane technique, is leaching from OE ?..nd OEE during iticubation in conventional \v aierbased media, as also observed in other ti.>.sues (13, I4-). To obtain inhibition of en-/\'t!e reactivity in the presetit study, it was, lioi^ever, necessary to preincubate sections \vi;h inhibitors diluted in buffer for 30 min prior to incubation; the membrane technique is not suitable for both preincubation and incubation procedures (14). As embryos older than 40 days furthermore had to be demineralized due to increasing hard tissue formation, fixation was foutid necessary.…”

Section: Discussionmentioning

confidence: 99%

Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Juhl

Vilmann

1990

European J Oral Sciences

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Juhl M, Vilmann H: Presence of a-naphthyl acetate esterase enzymes in dentai and oral epithelium of guinea pig embryos. Scand J Dent Res 1990; 98: 286-94. Abstract ~ This study examines the distribution of a-naphthyl acetate esterase enzymes in reduced enamel epithelium, i.e., post secretory ameloblasts (PSA) and external cells of reduced enamel epithelium (ERE) of continuously growing incisor and molar tooth germs and in the covering oral epithelium (OE). Jaws of guinea pig embryos, 25-50 days of gestation age, were pretreated, frozen, serially cut, and incubated with a-naphthyl acetate as substrate and hexazotized pararosaniline as eapSure agent for demonstration of enzyme activity. In addition, sections were preincubated with vatious inhibitors of enzyme activity. A strong enzyme reaction, essentially unaffected by pretreatment procedures, was detnonstrated in all cells of PSA and ERE, and in suprabasal cells of OE. Preliminary characterization ofthe a-naphthyl acetate esterase enzymes by means of inhibitors suggests a prevailing presence of B-esterase enzymes in both oral and dental epithelia. PSA were selectively stained when 2x10"^ M arsanilic acid was used as inhibitor, ERE were selectively inhibited by 10'^ M eserine, and cells of OE were selectively stained when 2 x 10"^ M HgClj was used as inhibitor. We therefore conclude that the a-naphthyl acetate esterase enzymes in combination with inhibitors may serve as histochemieal markers for discrimination of dental and oral epithelium in the guinea pig prior to eruption of teeth.

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Section: Discussionmentioning

confidence: 99%

Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Juhl

Vilmann

1990

European J Oral Sciences

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“…Aminopeptidase activity was demonstrated against L-leucine-4-methoxy-2-naphthylamide hydrochloride with Fast Blue B as the coupler. The substrate medium was as described by Wachsmuth & Wiist (1982) except that it contained 1% low gelling-temperature agarose (Sea Plaque) and was used in a semi-permeable membrane technique (Meijer & Vloedman, 1973).…”

Section: Histological and Enzyme Histochemical Techniquesmentioning

confidence: 99%

Some Histochemical Observations on Invasive Cells in a Myopathy Induced in Rats With 5–hydroxytryptamine

Christie

Modi

1984

Neuropathology Appl Neurobio

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The enzyme cytochemistry and proportion of mononuclear cells exclusively present within degenerating muscle fibres of the soleus of rats 24 h after a single intraperitoneal injection of 5-hydroxytryptamine (5-HT) (10 mg/kg) were studied. The following enzymes displayed strong cytochemical activities within these cells: non-specific esterase (against naphthol AS-D acetate and alpha-naphthylacetate), aminopeptidase (against leucine-4-methoxy-2-naphthylamide) and dipeptidyl peptidase II. A peroxidatic activity was also present, but was found to arise from cytochrome oxidase in phagocytosed muscle mitochondria. The aminopeptidase activity in the monocytic cells increased by roughly 50% between 6 and 24 h after 5-HT administration. Dipeptidyl peptidase II was found, by the use of appropriate inhibitors, to be present in both mononuclear cells and muscle fibres and may be implicated in degrading the basal lamina of muscle fibres, thereby permitting access to the mononuclear cells. The morphological, enzyme histochemical and electron microscopical evidence suggest that the cells which invade muscle fibres in the early stages of a myopathy induced by 5-HT in rats are actively phagocytosing, mature macrophages.

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“…Acid phosphatase activity was demonstrated histochemically by the method of Meyer ("1972). After I h incubation at 37'''C, 2% glutaraldehyde in cacodylate buffer (04 mol/l, pH 73) was used for postfixation, haematoxylin for counterstaining (15 min), and Euparal for mounting the slices.…”

Section: Enzyme Histochemistry On Human Hair Roots and Monkey Skin Bimentioning

confidence: 99%

Fabry's disease: biochemical and histochemical studies on hair roots for carrier detection

Vermorken

et al. 1978

Br J Dermatol

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A method of assay alpha-galactosidase and acid phosphatase activities in single hair roots is described. Enzyme histochemical studies show that the distribution of acid phosphatase in the human hair root matches that of alpha-galactosidase. Histochemically, the main activity is located in the upper part of the sheath near the orifice of the duct of the sebaceous gland. This is confirmed by enzyme assays on different parts of the hair root after dissection. The variation in the values found in individual hair roots is improved by relating alpha-galactosidase to acid phosphatase activities. Storage experiments indicate a remarkable stability of both alpha-galactosidase and acid phosphatase in human hair roots.

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Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections

Cited by 45 publications

References 13 publications

Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Some Histochemical Observations on Invasive Cells in a Myopathy Induced in Rats With 5–hydroxytryptamine

Fabry's disease: biochemical and histochemical studies on hair roots for carrier detection

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