Semiquantitative reverse transcription-polymerase chain reaction to evaluate the expression patterns of genes involved in the oestrogen pathway

Rey, JM; Pujol, Pascal; Callier, Patrick; Cavaillès, Vincent; Freiss, Gilles; Maudelondé, Thierry; Brouillet, JP

doi:10.1677/jme.0.0240433

Cited by 37 publications

(24 citation statements)

References 17 publications

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“…2A). FRO cells express predominantly S1P 2 , and ML-1 cells express several receptors for S1P (Balthasar et al 2006, Rey et al 2000. Furthermore, S1P and VEGF have been shown to cooperate in the regulation of cellular functions (Igarashi et al 2003, Fieber et al 2006.…”

Section: Resultsmentioning

confidence: 99%

“…Relative mRNA levels were calculated with the second derivative maximum method and LightCycler software. The data were normalized to the reference gene HPRT (Rey et al 2000). Control analyses, where reverse transcriptase was omitted from the cDNA synthesis reaction, were performed for each of the RNA preparations to ensure that the preparations were not contaminated with genomic DNA.…”

Section: Relative Quantitative Rt-pcr Of S1p Receptors and Vegfr-2mentioning

confidence: 99%

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Interactions between sphingosine-1-phosphate and vascular endothelial growth factor signalling in ML-1 follicular thyroid carcinoma cells

Balthasar¹,

Bergelin²,

Löf³

et al. 2008

Endocrine Related Cancer

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Sphingosine-1-phosphate (S1P) induces migration of human ML-1 thyroid follicular cancer cells and inhibits migration of human FRO anaplastic thyroid cancer cells. As tumour cells often secrete vascular endothelial growth factor (VEGF), we investigated a possible interaction between S1P and VEGF signalling in the regulation of thyroid tumour cell migration. We found that both ML-1 and FRO cells secreted VEGF-A (w3.6 and !0

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Section: Resultsmentioning

confidence: 99%

Section: Relative Quantitative Rt-pcr Of S1p Receptors and Vegfr-2mentioning

confidence: 99%

Interactions between sphingosine-1-phosphate and vascular endothelial growth factor signalling in ML-1 follicular thyroid carcinoma cells

Balthasar¹,

Bergelin²,

Löf³

et al. 2008

Endocrine Related Cancer

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“…We set up a multiplex RT-PCR strategy to quantify the cytokine mRNA levels relative to the hypoxanthine phosporibosyltransferase (HPRT) housekeeping gene, which was an internal control. 20 A typical experiment is shown in Figure 1. We noted large individual variations of all cytokine expression in healthy controls and genetically ascertained FMF patients (Figure 2a).…”

mentioning

confidence: 99%

Enhanced cytokine mRNA levels in attack-free patients with familial Mediterranean fever

et al. 2002

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Familial Mediterranean fever (FMF) is a recessively inherited inflammatory disorder, characterized by recurrent attacks of fever and serositis. Screening of mutations in the causing gene (MEFV) now allows accurate diagnosis of FMF among other inflammatory conditions. It is well documented that secreted levels of some pro-inflammatory cytokinesFamilial Mediterranean fever (FMF) is the prototype of a group of hereditary inflammatory disorders. Patients experience short, self resolving, attacks of fever and serosal inflammation (peritonitis, arthritis, pleuritis). 1 Although between attacks patients totally recover clinically, biological markers of inflammation (Serum Amyloid A protein, C Reactive Protein) sometimes remain elevated. 2,3 The gene for this recessive disorder (MEFV) was identified 4 years ago by a positional cloning strategy. 4,5 Screening for MEFV mutations has provided the first specific tool for diagnosing FMF, which can now be differentiated from the various other inflammatory conditions. However, it has been proposed that some MEFV variants may predispose to inflammation in general, [6][7][8] and indeed, preferential association with other inflammatory conditions has been reported. [9][10][11] The function of MEFV is still unknown. The disease phenotype and the almost exclusive expression of MEFV in leukocytes suggest that the corresponding protein plays an important role in the inflammatory cascade. [12][13][14] Some inflammatory mediators such as cytokines have been shown to be involved in the pathogenesis of FMF. [15][16][17][18] The cytokine network has been studied in many circumstances in clinically diagnosed patients, ie during and between attacks, both on and off colchicine treatment, and after ex vivo induction, as well as in controls. The secreted levels of pro-inflammatory cytokines are enhanced during attacks, whereas during attack-free periods they do not differ or are slightly higher than in healthy subjects.The present study is the first investigation of cytokines (i) at the transcriptional level, and (ii) in genetically ascertained patients. We have compared messenger RNA (mRNA) levels of four pro-inflammatory cytokines, tumor necrosis factor alpha (TNF-␣), interleukin 1 beta (IL-1␤), interleukin 6 (IL-6) and interleukin 8 (IL-8) in FMF patients in clinical remission and in healthy controls. TNF-␣ plays a key role in the inflammatory process by stimulating production of IL-1, which has a pyretic action; IL-6, promotes the release of acute phase reactants, and IL-8, is a chemoattractant.The FMF group comprised 44 patients, whose composition is shown in Table 1. 19 They were all in attack-free intervals upon blood sampling, based on clinical criteria: (i) no fever, no pain, no sign of inflammation, (ii) the time of their last attack was systematically documented, and happened at least 3 weeks earlier. The control group comprised 31 mutation-free healthy volunteers, unrelated to the patient group. We set up a multiplex RT-PCR strategy to quantify the cytokine mRNA levels relative to ...

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“…Expression of FAP-1, a negative regulator of Fas mediated apoptosis has also been investigated in endometrial cancer (Lee et al 1999, Rey et al 2000. However, the correlation between expression and response to therapy has yet to be investigated.…”

Section: P Chaudhry and E Asselin: Therapeutic Resistance In Endometrmentioning

confidence: 99%

Resistance to chemotherapy and hormone therapy in endometrial cancer

Chaudhry¹,

Asselin²

2009

Endocrine-Related Cancer

113

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Endometrial cancer is the most common gynecological malignancy in developed countries and represents the eighth leading cause of cancer related death in women. The growing incidence of endometrial cancer leads scientists and oncologists to identify effective preventive measures and also molecular markers for diagnosis and prognosis. Chemotherapy and hormone therapy is the mainstay treatment option for advanced and recurrent endometrial cancer and response to therapy is one of the most important factor which favors prognosis and overall survival. In recent years, there have been major advances in the treatment of patients with endometrial cancer. Despite advances made in the treatment of this cancer, the overall survival of patients has not significantly improved because considerable number of patients harbor tumor refractory to these therapies and the majority of the initially responsive tumors become refractory to treatments. Therefore, determination of sensitivity/resistance is becoming increasingly important for individualization of endometrial cancer therapy. The aim of this review is to present the existing knowledge about the molecular markers that could play a crucial role in determining resistance to chemo-and hormone therapy. Extensive literature search for the cell signaling pathways and factors responsible for chemoresistance have been performed and reviewed. Several recent studies suggest that deregulations in the apoptotic pathways (such as p53, Fas/FasL, Bcl-2 family proteins, inhibitor of apoptosis proteins), survival pathways (PI3K/AKT, MAPK), hormone receptor signaling pathways (progesterone receptor), Cyclooxygenase-2 and Her-2 are considered as key factors involved in the onset and maintenance of therapeutic resistance, suggesting that resistance is a multi-factorial phenomenon.

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Semiquantitative reverse transcription-polymerase chain reaction to evaluate the expression patterns of genes involved in the oestrogen pathway

Cited by 37 publications

References 17 publications

Interactions between sphingosine-1-phosphate and vascular endothelial growth factor signalling in ML-1 follicular thyroid carcinoma cells

Interactions between sphingosine-1-phosphate and vascular endothelial growth factor signalling in ML-1 follicular thyroid carcinoma cells

Enhanced cytokine mRNA levels in attack-free patients with familial Mediterranean fever

Resistance to chemotherapy and hormone therapy in endometrial cancer

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