Semisolid culture medium improves mycelial recovery of Agaricus subrufescens cryopreserved in cereal grains

Abstract

This study aimed to evaluate the effects of the solid and semisolid culture medium on the mycelial viability of A. subrufescens after 5-year cryopreservation at − 70°C. Mycelia were grown in three types of whole or ground grains, with or without 5% glycerol addition in the substrate and/or in a cryotube. After 5 years of cryopreservation at − 70°C, every treatment was thawed and recovered in malt extract culture medium with 15 (solid culture medium) or 5 g L −1 (semisolid culture medium) of agar. The semisolid recovery culture medium increased the mycelial viability recovery capacity of A. subrufescens cryopreserved for 5 years in grains with glycerol only in the cryotube, and specifically with medium-hard wheat grain without glycerol addition at all. Agar-based substrates such as malt extract agar, agar-ground grain, or the one with glycerol addition to the substrate were not effective to keep the mycelial viability, regardless of the recovery culture medium consistency. Hard and medium-hard endosperm wheat grains or hard endosperm rye grains with addition of glycerol as cryoprotectant only to the cryotube were effective to cryopreserve the fungus for 5 years without cryoprotectant addition in the substrate.

Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp

“…This study used Lentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) U9-1 strain from the Culture Collection of the Laboratory of Molecular Biology at Paranaense University, cryopreserved by the wheat grain technique, 28,29 identified by sequencing the internal transcribed spacers (ITS) of ribosomal DNA, and deposited in the GenBank database (http://www. ncbi.nlm.nih.gov/genbank/) with the accession number MG211674.…”

Lentinus crinitus basidiocarp stipe and pileus: chemical composition, cytotoxicity and antioxidant activity

“…Mushrooms are prized for their taste, nutritional value, and bioactive compounds used for health and well-being [1][2][3][4][5] but just some of them are cultivated in industrial scale worldwide such as Pleurotus, Lentinula, Auricularia, Agaricus, Flammulina, Coprinus, Agrocybe, and Volvariella genera [6][7][8][9][10][11][12][13].…”

HPTLC-Based Chemical Profiling: An Approach to Monitor Plant Metabolic Expansion Caused by Fungal Endophytes

“…Apart from their metabolic complexity, the metabolomic profiling of microorganisms has always been challenging due to the chemical noise caused by the components extracted from the culture medium [ 26 ]. This issue has been partially solved by sample cleanup procedures with specific resins like HP-20, solid phase extraction or liquid-liquid partitioning with organic solvents (ethyl acetate) [ 27 , 28 ]. However, for metabolomics purposes, this pre-purification might lead to the loss of important polar compounds aside from being time-consuming.…”