Semitransparent bandages based on chitosan and extracellular matrix for photochemical tissue bonding

Frost, Samuel J; Mawad, Damia; Wuhrer, Richard; Myers, Simon J.; Lauto, Antonio

doi:10.1186/s12938-018-0444-1

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…A relatively unexplored alternative is to harness both systems’ advantages to combine the microbiostatic material with microbiocidal nanopattern surface topography. There are very few reports of coatings that combine a cationic polymer and nanopatterned surface, which are also antimicrobial and biocompatible. , The combination of a microbiostatic material synergistically paired with a microbicidal nanopillar surface is a potentially effective, environmentally friendly, and long-lasting antimicrobial approach.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Antimicrobial Activity of a Nanopillar Surface on a Chitosan Hydrogel

Heedy

Marshall

Pineda

et al. 2020

ACS Appl. Bio Mater.

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Despite ongoing efforts and technology development, the contamination of medical device surfaces by disease-causing microbes remains problematic. Two approaches to producing antimicrobial surfaces are using antimicrobial materials and applying physical topography such as nanopatterns. In this work, we describe the use of physical topography on a soft hydrogel to control microbial growth. We demonstrate this approach by using chitosan hydrogel films with nanopillars having periodicities ranging from 300 to 500 nm. The flat hydrophilic chitosan films exhibit antimicrobial activity against the pathogenic bacteria Pseudomonas aeruginosa and filamentous fungi Fusarium oxysporum . The addition of nanopillars to the hydrogel surface further reduces the growth of P. aeruginosa and F. oxysporum up to ∼52 and ∼99%, respectively. Multiple modes of antimicrobial action appear to act synergistically to inhibit microbial growth on the nanopillar hydrogels. We verified that the strongly bactericidal and fungicidal nanopillared material retains biocompatibility to human epithelial cells with the MTT assay. The nanopillared material is a promising candidate for applications that require a biocompatible and antimicrobial film. The study demonstrates that taking advantage of multiple modes of antimicrobial action can effectively inhibit pathogenic microbial growth.

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Section: Introductionmentioning

confidence: 99%

Synergistic Antimicrobial Activity of a Nanopillar Surface on a Chitosan Hydrogel

Heedy

Marshall

Pineda

et al. 2020

ACS Appl. Bio Mater.

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“…Adding chitosan to solubilized dECM to create scaffolds can be used to add antimicrobial properties and to modify the mechanical properties and pore sizes of the scaffold [ 110 , 115 , 197 , 279 , 280 ]. Chitosan can also be added to dECM to allow for photo-cross-linking to tissues with the right cross-linking agent [ 281 ].…”

Section: Ecm Modification and Methodsmentioning

confidence: 99%

“…Photoinitiators also induce protein cross-linking, but their mechanism is to generate reactive intermediates that initiate the cross-linking. Many photoinitiators, such as Irgacure 2959 [ 139 , 168 ], riboflavin/vitamin B 2 [ 136 , 161 , 169 ], and rose bengal [ 281 ] require excitation with UV to initiate the cross-linking process. Since UV can be harmful to cells embedded in a scaffold, other photoinitiators that work with visible light, such as Eosin Y [ 237 ], are being used to cross-link solubilized dECM.…”

Section: Ecm Modification and Methodsmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.

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“…The porous bioelectronic patch was fabricated following our previously published protocol , and is schematically presented in Figure A. Briefly, 1.6 mL of a stock solution of chitosan (1.6 wt % in 2 v/v % acetic acid solution) was drop-cast on a microscope slide (75 mm × 26 mm) and left to dry at room temperature for 2 weeks. − …”

Section: Methodsmentioning

confidence: 99%

Impact of Sterilization on a Conjugated Polymer-Based Bioelectronic Patch

Yan

Travaglini

Lau

et al. 2021

ACS Appl. Polym. Mater.

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Organic bioelectronics based on conjugated polymers as the active electronic material have been shown to operate efficiently at the biointerface. Their translation into a commercial medical device will hinge on their long-term operation in vivo. This will require the device to be subjected to clinically approved sterilization techniques without deterioration in its physical and electronic properties. To date, there remains a gap in the literature addressing the impact of this critical preoperative procedure on the properties of conjugated polymers. This study aims to address this gap by assessing the physical and electronic properties of a sterilized porous bioelectronic patch having polyaniline as the conjugated polymer. The patch was sterilized by autoclave, ethylene oxide, and gamma (γ-) irradiation at 15, 25, and 50 kGy doses. Autoclaving resulted in cracking and macroscopic degradation of the patch, while patches sterilized by γ-irradiation at 50 kGy exhibited reduced mechanical and electronic properties, attributed to chain scission and nonuniform cross-linking caused by high dose irradiation. Ethylene oxide and γ-irradiation at 15 and 25 kGy sterilization appeared to be the most effective at maintaining the mechanical and electronic properties of the patch and inducing a minimal immune response as revealed by a receding fibrotic capsule after 4 week implantation. Our findings pave the way toward closing the gap for the translation of organic bioelectronic devices from acute to long-term in vivo models.

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Semitransparent bandages based on chitosan and extracellular matrix for photochemical tissue bonding

Cited by 8 publications

References 35 publications

Synergistic Antimicrobial Activity of a Nanopillar Surface on a Chitosan Hydrogel

Synergistic Antimicrobial Activity of a Nanopillar Surface on a Chitosan Hydrogel

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Impact of Sterilization on a Conjugated Polymer-Based Bioelectronic Patch

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