Senescence-associated β-galactosidase—A biomarker of aging, DNA damage, or cell proliferation restriction?

Моргунова, Г. В.; Колесников, А. Ф.; Клебанов, А. А.; Хохлов, А. Н.

doi:10.3103/s0096392515040082

Cited by 14 publications

(11 citation statements)

References 18 publications

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“…Substrates of this reaction are β- d -galactosides, such as lactose, keratin sulfates, sphingolipids, etc. [ 2 ]. Highly concentrated enzyme can cause disintegration of macromolecular proteoglycans and damage basement membranes through hydrolyzation of glycosidic bonds, resulting in separation of side chains of amino polysaccharides from core proteins [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Senescence-Associated β-Galactosidase Detection in Pathology

et al. 2022

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Activity of β-galactosidase at pH 6 is a classic maker of senescence in cellular biology. Cellular senescence, a state of highly stable cell cycle arrest, is often compared to apoptosis as an intrinsic tumor suppression mechanism. It is also thought that SA-β-gal is crucial in malignant cell transformation. High levels of senescence-associated β-galactosidase (SA-β-gal) can be found in cancer and benign lesions of various localizations making the enzyme a highly promising diagnostic marker for visualization of tumor margins and metastases. These findings facilitate the research of therapy induced senescence as a promising therapeutic strategy. In this review, we address the need to collect and analyze the bulk of clinical and biological data on SA-β-gal mechanisms of action to support wider implementation of this enzyme in medical diagnostics. The review will be of interest to pathologists, biologists, and biotechnologists investigating cellular senescence for purposes of regenerative medicine and oncology.

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Section: Introductionmentioning

confidence: 99%

Senescence-Associated β-Galactosidase Detection in Pathology

et al. 2022

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“…SA-β-gal is a DNA damaging enzyme that is found only in senescent cells. 26,27 HPL did not cause any morphological abnormalities in T47D cells. Cells propagated using HPL had normal morphology compared to control cells and cells cultured in FBS.…”

Section: Discussionmentioning

confidence: 70%

Utilization of Expired Platelet Concentrate for Production of Human Platelet Lysate as a Medium for T47D Cell Propagation

Mentari

Pebrina

Nurpratami³

2022

Mol Cell Biomed Sci

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Background: Platelet concentrate (PC) has a short shelf life (5 days). Expired PC cannot be used for clinical purposes. PC is used for human platelet lysate (HPL) production, which was found to be more effective than FBS at increasing T47D cell proliferation. HPL production using expired PC has not been reported. This study aimed to investigate whether the use of HPL produced from expired PC (storage duration >5 days) can increase the proliferation of T47D cells in vitro.Materials and methods: Expired PC samples with a shelf life of 7 and 11 days were used to produce HPL via freeze/thaw method. pH, total protein content, glucose and albumin levels were measured. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure proliferation rate and doubling time of HPL-treated T47D cells.Results: After HPL production, the glucose level was influenced by the pH (p=0.003), and albumin level was influenced by total protein content (p=0.030). HPL stored for 7 and 11 days increased cell proliferation rate by 1.41 and 1.80 times higher than 10% FBS, respectively. HPL produced from expired PC did not cause morphological abnormality of the cells. In this study, the glucose levels affected cell proliferation (p=0.030). High glucose levels inhibited T47D cell proliferation.Conclusion: Expired PC can be used as a potential material for HPL production, since HPL produced from expired PC increases cell proliferation rate and shortens cell doubling time.Keywords: cell proliferation, human platelet lysate, platelet concentrate, thrombocyte, T47D

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“…The proportion of SA-βgal positive cells, an indicator of cellular senescence, was not different between the two groups (Fig. 1 f ) (Morgunova et al 2016 ). To evaluate the conditions of the cells, we conducted cell cycle analysis following previous reports (Yang et al 2006 ; Son et al 2021 ).…”

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confidence: 82%