Senescence-specific change in ROS scavenging enzyme activities and regulation of various SOD isozymes to ROS levels in psf mutant rice leaves

Wang, Fubiao; Liu, Jianchao; Zhou, Lujian; Pan, Gang; Li, Zhaowei; Zaidi, Syed Hassan Raza; Cheng, Fangmin

doi:10.1016/j.plaphy.2016.10.005

Cited by 105 publications

(62 citation statements)

References 36 publications

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“…Then, GSH is synthesized from the intermediate products γ-glutamylcysteine and glycine with GSH2 as the catalyst; GSH1 is a key and rate-limiting enzyme in GSH biosynthesis, although some studies have shown that GSH2 is also rate limiting for GSH synthesis. Regardless, GSH1 and GSH2 are important enzymes for GSH synthesis and provide feedback on GSH activity (Wang et al, 2016a). γ-Glutamylcysteine synthetase is also a rate-limiting enzyme in GSH synthesis that determines the GSH level in vivo and is an important antioxidant factor.…”

Section: Discussionmentioning

confidence: 99%

Preventive effect of Lactobacillus plantarum KSFY02 isolated from naturally fermented yogurt from Xinjiang, China, on d-galactose–induced oxidative aging in mice

Zhao

Zhou

et al. 2019

Journal of Dairy Science

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Yogurt from Xinjiang, China, is a traditional and naturally fermented food, and abundant microorganisms are produced during its fermentation process. In this study, we carried out in vivo animal experiments to explore the effect of a newly isolated lactic acid bacterial strain, Lactobacillus plantarum KSFY02 (LP-KSFY02), on oxidative aging. We used d-galactose to induce oxidative aging in mice and analyzed the serum and tissues of those mice using molecular biology detection methods. The results showed that LP-KSFY02 could inhibit the decreases in the thymic, cerebral, cardiac, liver, spleen, and kidney indices of mice caused by oxidative aging. The LP-KSFY02 strain increased activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and glutathione (GSH) and reduced levels of nitric oxide (NO) and malondialdehyde in the serum, liver, and spleen of the oxidative aging mice. Pathological observation demonstrated that LP-KSFY02 alleviated damage to the liver and spleen of oxidative aging mice. Quantitative PCR showed that LP-KSFY02 effectively upregulated mRNA expression of neuronal nitric oxide synthase (Nos1), endothelial nitric oxide synthase (Nos3), copper/zinc superoxide dismutase (Sod1), manganese superoxide dismutase (Sod2), catalase (Cat), heme oxygenase-1 (Hmox1), nuclear factor erythroid 2 related factor 2 (Nfe2l2), γ-glutamylcysteine synthetase (Gclm), and quinone oxidoreductase 1 (Nqo1) in mouse liver and spleen and downregulated expression of inducible nitric oxide synthase (Nos2). Western blot analysis revealed that LP-KSFY02 effectively upregulated protein expression of SOD1, SOD2, CAT, GSH1, and GSH2 in mouse liver and spleen tissues. Therefore, LP-KSFY02 can effectively prevent d-galactose-induced oxidative aging in mice. Its efficacy was superior to that of Lactobacillus delbrueckii ssp. bulgaricus (LDSB) and vitamin C, which are commonly used in the medical field as antioxidants. Thus, LP-KSFY02 is a high-quality strain with probiotic potential.

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Section: Discussionmentioning

confidence: 99%

Preventive effect of Lactobacillus plantarum KSFY02 isolated from naturally fermented yogurt from Xinjiang, China, on d-galactose–induced oxidative aging in mice

Zhao

Zhou

et al. 2019

Journal of Dairy Science

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“…Thus, the NSCs in leaf sheaths might play an important compensating role in grain filling when the functional leaves experienced early aging during grain filling (Li et al., ). For different functional leaves within a rice plant, the upper‐positional leaves differed from the lower leaves in their photosynthetic assimilation abilities as well as the initiation and progression of leaf senescence during grain filling (Wang et al., ). Nevertheless, there are few reports about the relationship of leaf senescence induced by N deficiency with the accumulation and translocation of NSC in leaf sheaths for different sheath positions within a rice plant.…”

Section: Introductionmentioning

confidence: 99%

Senescence‐related translocation of nonstructural carbohydrate in rice leaf sheaths under different nitrogen supply

et al. 2020

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The translocation of nonstructural carbohydrates (NSC) from leaf sheaths to filling grains after anthesis contributed greatly to the grain yield of cereal crops. In this study, the effect of nitrogen (N) supply levels on the accumulation and translocation of NSC in leaf sheath tissues and its relationship with the initiation and progression of leaf senescence during grain filling was investigated using two rice (Oryza sativa L.) genotypes, namely, premature flag leaf senescence mutant (psf) and its wild‐type. Three N treatment levels were used to examine N‐supply induced alteration in the activities of several key enzymes involved in NSC translocation and N assimilation in different leaf sheaths. The results show that the NSC translocation rate in leaf sheaths under low nitrogen (LN) treatment was significantly higher than those under normal nitrogen (NN) and high nitrogen (HN) treatments. However, the positive effect of LN on the NSC translocation in leaf sheath was closely associated with its negative effect on grain yield, due to accelerated leaf senescence and shortened leaf longevity. Comparatively, the upper‐positional sheath had a lower NSC amount and higher NSC translocation rate than the lower‐leaf sheaths after heading. High N suppressed sucrose‐phosphate synthase (SPS) activity in leaf sheaths, but enhanced the activity of key enzymes involving in N assimilation in leaf sheaths. The upper sheath had higher activity of sucrose‐metabolizing enzymes and lower activity of N‐assimilating enzymes. Hence, the upper‐leaf sheath had a relatively weak N assimilation and stronger NSC translocation than the lower‐leaf sheaths.

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“…production, and the outbreak of reactive oxygen species is known to lead to leaf senescence (Edreva, 2005). The excessive accumulation of these reactive oxygen species on the plant has a particularly toxic effect, influencing the process of intracellular metabolism, causing membrane lipid peroxidation, leading to cell membrane and endometrial system damage, and further affecting the rice leaves before programmed cell death, leading to plant senescence (Wang et al, 2016). Superoxide dismutase, as an O 2 − scavenging factor, can convert O 2 − into H 2 O 2 by disproportionation, and excess H 2 O 2 is decomposed by POD into water, which is completely harmless (Alscher et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…At this time, O 2 is used as an electron acceptor to form O 2 − , and O 2 − initiates a series of chain reactions to produce more H 2 O 2 and ×OH (Chow et al, 2013). The excessive accumulation of these reactive oxygen species on the plant has a particularly toxic effect, influencing the process of intracellular metabolism, causing membrane lipid peroxidation, leading to cell membrane and endometrial system damage, and further affecting the rice leaves before programmed cell death, leading to plant senescence (Wang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Identification and Gene Mapping of an Early Senescent Leaf Mutant esl11 of Rice

Wang

Xie

et al. 2018

Crop Science

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As an essential process in the life of a plant, natural leaf senescence is necessary for adaptation to environmental diversity, whereas early leaf senescence can reduce per‐unit yield and cause inferior harvest quality during crop production. To explore the molecular mechanism of leaf senescence in rice (Oryza sativa L.), we studied an early senescence leaf (esl11) mutant obtained by ethyl methane sulfonate (EMS) mutagenesis. The leaves of the esl11 mutant showed normal growth and leaf coloration prior to the three‐leaf stage, but after this stage, all fully expanded leaves underwent yellowing and senescence from the edges of the leaf blade and tips, apart from the emerging leaves, which showed gradual growth. The net photosynthetic rate and photosynthetic pigment content of the tip and leaf base of the mutant esl11 were significantly lower than those of the wild type. The role of active oxygen species O2−, H2O2, and ⋅OH in the mutant increased, whereas the activities of MDA, peroxidase, superoxide dismutase, and catalase decreased compared with the wild type. The results of trypan blue staining on the senescent part of the leaf tip of the mutant esl11 indicated procedural death in part of the cell, whereas the wild type showed no change. The early leaf senescence trait in the mutant was controlled by of recessive nuclear gene, which was mapped to chromosome 7, with a physical range of 143.0 kb. The results of this study provide the foundation for important progress in gene cloning and functional analysis of the esl11 gene.

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Senescence-specific change in ROS scavenging enzyme activities and regulation of various SOD isozymes to ROS levels in psf mutant rice leaves

Cited by 105 publications

References 36 publications

Preventive effect of Lactobacillus plantarum KSFY02 isolated from naturally fermented yogurt from Xinjiang, China, on d-galactose–induced oxidative aging in mice

Preventive effect of Lactobacillus plantarum KSFY02 isolated from naturally fermented yogurt from Xinjiang, China, on d-galactose–induced oxidative aging in mice

Senescence‐related translocation of nonstructural carbohydrate in rice leaf sheaths under different nitrogen supply

Identification and Gene Mapping of an Early Senescent Leaf Mutant esl11 of Rice

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