Senescent Profile of Cryopreserved Adipose Mesenchymal Stem Cells After Multiple Passages

Arif, Starifulkani; Dilogo, Ismail Hadisoebroto; Pawitan, Jeanne Adiwinata; Anggraeni, Ria; Gani, Yogi Ismail

doi:10.1166/asl.2017.9434

Cited by 1 publication

(1 citation statement)

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“…The issue of repeated cryopreservation, as well as the effects of multiple freeze–thaw cycles is of equal importance. Recent research shows that early passage cultures can be frozen and thawed up to two times without any loss of activity or functionality [ 88 ]. However, four or more freeze–thaw cycles cause both early aging and a decrease in the MSCs’ proliferative activity, which may be related to accumulated stress impact from the multiple cryopreservations.…”

Section: Cryopreservation Of Msc Suspensionsmentioning

confidence: 99%

Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products

Linkova

Rubtsova

Egorikhina

2022

Cells

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Mesenchymal stem cells (MSCs) manifest vast opportunities for clinical use due both to their ability for self-renewal and for effecting paracrine therapeutic benefits. At the same time, difficulties with non-recurrent generation of large numbers of cells due to the necessity for long-term MSC expansion ex vivo, or the requirement for repeated sampling of biological material from a patient significantly limits the current use of MSCs in clinical practice. One solution to these problems entails the creation of a biobank using cell cryopreservation technology. This review is aimed at analyzing and classifying literature data related to the development of protocols for the cryopreservation of various types of MSCs and tissue-engineered structures. The materials in the review show that the existing techniques and protocols for MSC cryopreservation are very diverse, which significantly complicates standardization of the entire process. Here, the selection of cryoprotectors and of cryoprotective media shows the greatest variability. Currently, it is the cryopreservation of cell suspensions that has been studied most extensively, whereas there are very few studies in the literature on the freezing of intact tissues or of tissue-engineered structures. However, even now it is possible to develop general recommendations to optimize the cryopreservation process, making it less traumatic for cells.

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Section: Cryopreservation Of Msc Suspensionsmentioning

confidence: 99%