Sensibilidade Da Coloração De Gram No Diagnóstico Prévio Das Infecções Em Sítios Corporais Estéreis

Abstract

O isolamento e identificação do agente etiológico causador de infecção a partir de líquidos biológicos podem ser um fator crítico para a recuperação da saúde do paciente, pois estas infecções geralmente são graves e deixam seqüelas. Neste trabalho foram analisadas 82 amostras de diferentes líquidos biológicos, comparando os resultados da coloração de Gram com os das culturas, além de verificar incidência dos microrganismos isolados. O Gram apresentou sensibilidade e especificidade de 62,5% e 93,9% respectivamente. Pseudomonas aeruginosa, Escherichia coli e Staphylococcus aureus foram as bactérias mais isoladas. Concluiu-se que a Coloração de Gram pode ser uma ferramenta útil na análise dos líquidos biológicos, contribuindo no diagnóstico preliminar destas infecções. SENSITIVITY OF GRAM STAINING FOR EARLY DIAGNOSTIC OF INFECTIONS IN STERILE BODY SITES Abstract Isolation and identification of an etiologic agent from biological fluids can be a critic factor for the clinical outcome of the patient, because this infection can generally be severe and cause sequels. Eighty-two different samples of biological fluids were analyzed through the Gram staining method. The results of Gram were compared with those obtained with culture, and the incidence of isolated microorganisms was also analyzed. The Gram staining presented 62.5% and 93.9% of sensitivity and specificity, respectively. Among all types of isolated and identified bacteria, P. aeruginosa, E. coli and S. aureus were the commonest ones. It was concluded that the Gram staining could be a useful tool on analysis of biological fluids, contributing for the previous diagnostic of these infections.

Enrichment methodology to increase the positivity of cultures from body fluids

“…However, the risk of false negative results is high because only a small number of microorganisms may be present in the specimens [3,4]. In an attempt to overcome this drawback, innovative techniques using enrichment media and newly-developed laboratory devices and instruments, such as blood culture bottles, have been adopted [5]. We evaluated the positivity of various biological body fluids, comparing the results obtained with the conventional method with those produced with the enrichment method.…”