Sensing and amplification of oligonucleotide-DNA interactions by means of impedance spectroscopy: a route to a Tay–Sachs sensor

Bardea, Amos; Patolsky, Fernando; Dagan, Arie; Willner, Itamar

doi:10.1039/a808319c

Cited by 200 publications

(114 citation statements)

References 17 publications

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“…A hybridisation efficiency lower than 10% was observed. The 18-mer probes, containing five thiophosphate thymines, were also used by Willner and co-workers (Patolsky et al, 1999;Bardea et al, 1999b;Alfonta et al, 2001). The 18-mer oligonucleotides, modified by a phosphorothioate group either at its 3 or both 3 and 5 terminals, were used by Hianik and co-workers to modify gold electrodes (Hianik et al, 2001).…”

Section: Direct Self-assembly Of Phosphorothioester-modified Probesmentioning

confidence: 99%

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Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Lucarelli

Marrazza

Turner

et al. 2004

Biosensors and Bioelectronics

374

204

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Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples.

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Section: Direct Self-assembly Of Phosphorothioester-modified Probesmentioning

confidence: 99%

“…A novel method for the electronic transduction of DNA hybridisation, based on faradic impedance spectroscopy, was reported by Willner and co-workers (Bardea et al, 1999b). Impedance spectra were recorded in the presence of [Fe(CN) 6 ] 3−/4− redox probe.…”

Section: Enzyme Labelsmentioning

confidence: 99%

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Lucarelli

Marrazza

Turner

et al. 2004

Biosensors and Bioelectronics

374

204

View full text Add to dashboard Cite

show abstract

“…Electroless deposition of silver onto metallic nanoparticle labels has also been used [37,[79][80][81]. Various methods have been reported for post-affinity attachment of charged and/or bulky particles that alter the interface impedance [82][83][84][85].…”

Section: Amplificationmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

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“…In the presence of a redox probe, the sensor probes the biological events occurring on its surface by measuring the induced changes in Faradaic impedance. This technique has been regarded as an effective way to sense the formation of antigen-antibody (Kharitonov et al, 2000;Maupas et al, 1996;Patolsky et al, 1998;DeSilva et al, 1995), biotin-avidin complexes (Athey et al, 1995), oligonucleotide-DNA interaction (Bardea et al, 1999), and the binding of foot-and-mouth disease virus (Rickert et al, 1996) on electrode surfaces by probing the features of the interfacial properties (capacitance, electron transfer resistance) of electrodes.…”

Section: Impedance Biosensors For Bacterial Detectionmentioning

confidence: 99%

Food‐borne Pathogenic Bacteria

Shi

Xie

Zhou

2017

Food Safety in China

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The realization of rapid, sensitive, and specific methods to detect foodborne pathogenic bacteria is central to implementing effective practice to ensure food safety and security. As a principle of transduction, the impedance technique has been applied in the field of microbiology as a means to detect and/or quantify foodborne pathogenic bacteria. The integration of impedance with biological recognition technology for detection of bacteria has led to the development of impedance biosensors that are finding wide-spread use in the recent years. This paper reviews the progress and applications of impedance microbiology for foodborne pathogenic bacteria detection, particularly the new aspects that have been added to this subject in the past few years, including the use of interdigitated microelectrodes, the development of chip-based impedance microbiology, and the use of equivalent circuits for analysis of the impedance systems. This paper also reviews the significant developments of impedance biosensors for bacteria detection in the past 5 years, focusing on microfabricated microelectrodes-based and microfluidic-based Faradaic electrochemical impedance biosensors, non-Faradaic impedance biosensors, and the integration of impedance biosensors with other techniques such as dielectrophoresis and electropermeabilization. Published by Elsevier Inc.

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Sensing and amplification of oligonucleotide-DNA interactions by means of impedance spectroscopy: a route to a Tay–Sachs sensor

Cited by 200 publications

References 17 publications

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Label‐Free Impedance Biosensors: Opportunities and Challenges

Food‐borne Pathogenic Bacteria

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