Sensing and imaging of PPi <i>in vivo</i> using lanthanide-based second near-infrared luminescent probes

Su, Yaoquan; Ye, Lingyue; Gu, Jiayu; Zhao, Lingzhi; Zhou, Yunyun; Peng, Juanjuan

doi:10.1039/d1tb02579a

Cited by 10 publications

(3 citation statements)

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“…Article https://doi.org/10.1038/s41467-024-45052-1 more sensitive toward the dielectric environment of SWNTs such as absolute PL intensities and PL peak positions will require even more advanced adjustments. A previously reported NIR-II fluorescent sensor for PP i based on lanthanide nanoparticles showed similar sensitivity and higher selectivity toward PP i but lacked the advantage of internal calibration and multimodal sensing enabled by Dz-alkyne functionalized SWNTs 39 .…”

Section: Detection Of Pp I In Biological Environmentsmentioning

confidence: 99%

“…In recent years, in vivo imaging within the NIR-II has emerged as a method that benefits from ultralow light scattering and deeper penetration through biological tissues 34,35 . Various NIR-II fluorescent probes have been developed, including organic dyes 36,37 , gold nanoclusters 38 , quantum dots and lanthanide nanocrystals [39][40][41] . However, they often suffer from limited biocompatibility due to the presence of toxic transition metals (e.g., Pb, Cd) or limited stability due to photobleaching 42,43 .…”

mentioning

confidence: 99%

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Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Settele,

Schrage,

Jung

et al. 2024

Nat Commun

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Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors.

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Section: Detection Of Pp I In Biological Environmentsmentioning

confidence: 99%

mentioning

confidence: 99%

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Settele,

Schrage,

Jung

et al. 2024

Nat Commun

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“…As a biologically active anion, inorganic pyrophosphate (PPi) is closely related to various cellular processes and metabolism [20]. In living organisms, PPi is produced by the hydrolysis of adenosine triphosphate (ATP) and participates in the polymerization of DNA and RNA [21][22][23]. In addition, PPi has been reported to be applied in the diagnosis of arthritis, chondrocalcinosis, and Mönckeberg's arteriosclerosis wildly, resulting from its intimate relationship with blood coagulation [24,25].…”

Section: Introductionmentioning

confidence: 99%

Thienopyrimidine‐derived multifunctional fluorescence sensor for the detection of Cu²⁺, Fe³⁺, and PPi in different solvents

Shi,

Kang,

Liu

et al. 2024

Luminescence

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Hydrazine substituted thienopyrimidine, a new fluorophore, was used to synthesize a novel Schiff base R1 as a chemosensor via the condensation with p‐formyltriphenylamine, and the structure was confirmed using nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) analysis. When treated with Cu2+ in dimethylsulfoxide (DMSO)/H2O buffer, R1 showed a phenomenon of fluorescence quenching, which was reversible with the action of ethylenediaminetetraacetic acid (EDTA). When treated with Fe3+ in dimethylformamide (DMF)/H2O buffer, R1 exhibited the same phenomenon, but fluorescence was recovered with inorganic pyrophosphate (PPi) quantitatively. The complexation ratios for R1‐Cu2+ and R1‐Fe3+ were both 1:2, which were manifested by MS titrations and corresponding Job's plots. The limits of detection of R1 for Cu2+ and Fe3+ were 3.11 × 10−8 and 1.24 × 10−7 M, respectively. The sensing mechanism of R1 toward Cu2+ and Fe3+ was confirmed using density functional theory calculations and electrostatic potential analysis. Test strips of R1 were fabricated successfully for on‐site detection of Cu2+ and Fe3+. In addition, R1 was applied to recognize Cu2+ and Fe3+ in actual water samples with satisfactory recovery.

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Ratiometric Fluorescence Assay for Pyrophosphate Based on Sulfur Nanodots Decorated Metal‐Organic Frameworks

Wang

et al. 2023

Chemistry A European J

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Selective and sensitive detection of inorganic pyrophosphate (PPi) are of great significance both for clinical applications and fundamental research. In this work, a ratiometric fluorescent probe was developed by decorating a porphyrin‐based metal‐organic framework (PCN‐224) with sulfur nanodots (S‐dots). The red‐fluorescence of PCN‐224 was significantly promoted (more than 6‐fold) by S‐dots through inhibiting molecular motion of porphyrin ligand, which also provided active sites for the detection of Cu2+ and PPi. Cu2+ could selectively quench the red‐fluorescence of PCN‐224 through coordination with porphyrin ligand, and the competition reaction between PPi and Cu2+ resulted in the decomposing of coordination and recovery of red‐fluorescence. The blue‐fluorescence from S‐dots was deemed as effective reference, which provided a built‐in correction in complex environments. Based on these photophysical properties, a ratiometric fluorescence assay was developed for the quantitative detection of Cu2+ and PPi, with a limit of detection of 0.11 and 2.66 μM, respectively. The accuracy and practical applications of assays were also demonstrated by detection in tap water and human serum samples.

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Sensing and imaging of PPi in vivo using lanthanide-based second near-infrared luminescent probes

Abstract: pathological changes. Here, we reported a luminescent nanoprobe for the detection and imaging of PPi in vivo based on a lanthanide nanoparticle with luminescence at the second near-infrared window modified...

Cited by 10 publications

References 44 publications

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Thienopyrimidine‐derived multifunctional fluorescence sensor for the detection of Cu²⁺, Fe³⁺, and PPi in different solvents

Ratiometric Fluorescence Assay for Pyrophosphate Based on Sulfur Nanodots Decorated Metal‐Organic Frameworks

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Sensing and imaging of PPi in vivo using lanthanide-based second near-infrared luminescent probes

Abstract: pathological changes. Here, we reported a luminescent nanoprobe for the detection and imaging of PPi in vivo based on a lanthanide nanoparticle with luminescence at the second near-infrared window modified...

Cited by 10 publications

References 44 publications

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Thienopyrimidine‐derived multifunctional fluorescence sensor for the detection of Cu2+, Fe3+, and PPi in different solvents

Ratiometric Fluorescence Assay for Pyrophosphate Based on Sulfur Nanodots Decorated Metal‐Organic Frameworks

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Thienopyrimidine‐derived multifunctional fluorescence sensor for the detection of Cu²⁺, Fe³⁺, and PPi in different solvents