Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes

Gomes, Rui S.; Moreira, Felismina T.C.; Fernandes, Rúben; Sales, M. Goreti F.

doi:10.1371/journal.pone.0196656

Cited by 47 publications

(30 citation statements)

References 47 publications

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“…The time given for incubation was typically set from 15 to 30 min. In general, longer times may improve sensitivity as there is more time to allow Cys-C adsorption, while shorter times may improve selectivity as there is little time for non-specific adsorption [33,34]. Therefore, as a compromise, without further experiments for this selection, the incubation time was set to an intermediate value of 20 min.…”

Section: Analytical Performance Of the Sensormentioning

confidence: 99%

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Plastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed Electrodes for Cystatin C Detection

et al. 2021

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This work reports the design of a novel plastic antibody for cystatin C (Cys-C), an acute kidney injury biomarker, and its application in point-of-care (PoC) testing. The synthetic antibody was obtained by tailoring a molecularly imprinted polymer (MIP) on a carbon screen-printed electrode (SPE). The MIP was obtained by electropolymerizing pyrrole (Py) with carboxylated Py (Py-COOH) in the presence of Cys-C and multiwall carbon nanotubes (MWCNTs). Cys-C was removed from the molecularly imprinted poly(Py) matrix (MPPy) by urea treatment. As a control, a non-imprinted poly(Py) matrix (NPPy) was obtained by the same procedure, but without Cys-C. The assembly of the MIP material was evaluated in situ by Raman spectroscopy and the binding ability of Cys-C was evaluated by the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) electrochemical techniques. The MIP sensor responses were measured by the DPV anodic peaks obtained in the presence of ferro/ferricyanide. The peak currents decreased linearly from 0.5 to 20.0 ng/mL of Cys-C at each 20 min successive incubation and a limit of detection below 0.5 ng/mL was obtained at pH 6.0. The MPPy/SPE was used to analyze Cys-C in spiked serum samples, showing recoveries <3%. This device showed promising features in terms of simplicity, cost and sensitivity for acute kidney injury diagnosis at the point of care.

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Section: Analytical Performance Of the Sensormentioning

confidence: 99%

“…tion [33,34]. Therefore, as a compromise, without further experiments for this selection, the incubation time was set to an intermediate value of 20 min.…”

Section: Analytical Performance Of the Sensormentioning

confidence: 99%

Plastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed Electrodes for Cystatin C Detection

et al. 2021

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“…They used label-free impedance to detect VEGF with a dynamic range from 20 to 200 pg mL −1 and a limit of detection of 0.08 pg mL −1 [ 146 ] ( Figure 11 ). Gomes et al developed an electropolymerized SPE with amine-substituted benzene rings as monomers and specifically charged monomers for creating protein imprinting materials for breast cancer biomarker CA 15-3 [ 147 ]. The commercial Au SPE from DropSens consists of gold working and auxiliary electrodes, and reference and electrical contacts made of silver.…”

Section: Screen Printingmentioning

confidence: 99%

“…The commercial Au SPE from DropSens consists of gold working and auxiliary electrodes, and reference and electrical contacts made of silver. Using this sensor, Gomes et al demonstrated a linear dynamic range of 0.25 to 20.00 U mL −1 , with detection limit of 0.05 U mL −1 , for CA 15-3, from square wave voltammograms in diluted serum, with only 15 min incubation between sensor and solution of CA-15-3 [ 147 ].…”

Section: Screen Printingmentioning

confidence: 99%

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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“…The electrically conductive nature of noble metal nanoparticles offers the possibility of combining electrochemical and optical sensing capabilities on a single substrate, and in combination with transparent conducting oxides (TCOs) allow for convenient optical studies of electrochemically induced perturbations in resonance conditions [9,10]. Electrochemical processes at metallic surfaces, including micro and nanostructures, are advantageous in the preparation of sensing devices with polymeric ligands (e.g., molecular imprint polymers-MIP) [11,12]. MIP layers can be prepared at plasmonic surfaces by bulk polymer synthesis [13] but control of film growth is difficult.…”

Section: Introductionmentioning

confidence: 99%

Under-Etched Plasmonic Disks on Indium Tin Oxide for Enhanced Refractive Index Sensing on a Combined Electrochemical and Optical Platform

2020

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A simple approach to enhance the refractive index sensitivity of gold nanodisks immobilized on electrically conducting indium tin oxide (ITO) substrates has been demonstrated. A two-fold increase in sensitivity to bulk refractive index change was achieved by substrate under-etching of gold nanodisks on ITO in 50 mM sulfuric acid. The influence of an intermediate titanium adhesion layer was investigated and was found to markedly influence the etching pattern and time. Etching with an adhesion layer resulted in enhanced refractive index sensitivity on disk-on-pin like structures after long etching times, whereas etching of disks deposited directly on ITO resulted in a disk-on-pincushion like configuration and similarly enhanced sensitivity already at shorter times. The gold disks remained electrically connected to the ITO substrate throughout etching and allowed site-specific electrodeposition of poly(3-aminophenol) at the nanodisks, showing enhanced thin-film refractive index sensitivity. This work demonstrates a simple method for enhancing refractive index sensitivity of nanostructures on ITO substrates for combined electrochemical and optical platforms, and subsequently a method to modify the surface of the electrically connected nanostructures, which has potential application in biosensing.

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Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes

Cited by 47 publications

References 47 publications

Plastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed Electrodes for Cystatin C Detection

Plastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed Electrodes for Cystatin C Detection

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

Under-Etched Plasmonic Disks on Indium Tin Oxide for Enhanced Refractive Index Sensing on a Combined Electrochemical and Optical Platform

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