Sensing mammographic density using single-sided portable Nuclear Magnetic Resonance

Alqurashi, Maher; Momot, Konstantin I.; Aamry, Ali; Al-Mohammed, H.I.; Aamri, Hussin; Johary, Yehia H.; Abolaban, Fouad A.; Sulieman, Abdelmoneim

doi:10.1016/j.sjbs.2021.12.022

Cited by 3 publications

(4 citation statements)

References 30 publications

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“…The suitability of single-sided Portable NMR for compositional and microstructural profiling of biological tissues has been well-demonstrated [7,56]. We have previously demonstrated that single-sided Portable NMR measurements of MD of patient-derived explants (PDEs) in vitro closely correlate with the MD measured by MicroCT and X-ray mammography [2,3,32,54], and Portable NMR measurements in healthy human volunteers in vivo enabled depth profiling of MD within the breast [40]. We have also used Portable NMR to accurately measure MD in PDEs of human breast tissue cultured ex vivo over two weeks [33].…”

Section: Introductionmentioning

confidence: 94%

Portable NMR for the investigation of models of mammographic density ex vivo: Androgens antagonise the promotional effect of oestrogen

Huang,

Reye,

Momot

et al. 2023

BSI

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Background: Increased mammographic density (MD) is a strong and independent risk factor for breast cancer. Lifetime oestrogenic exposure is associated with increased MD, however androgenic effects on MD have not been widely investigated. Methods: We studied the effect of 17 β-oestradiol (E2) alone or in combination with an androgen receptor (AR) agonist ( 5 α-dihydrotestosterone [DHT]) or a selective AR modulator (Enobosarm), in modulating MD as measured via single-sided Portable NMR in a patient-derived explant (PDE) model of normal human mammary tissue. Results: We observed an upward trend in explants treated with E2 alone in 3/6 cases, an effect which appeared to be somewhat influenced by menopausal status. Co-treatment of E2 with the AR agonists DHT or Enobosarm however effected a downward trend in regards to MD. E2 significantly upregulated the ER regulated genes (ERGs) CELSR2 and AR, and the AR regulated genes SEC14L2 and GRPC5A, whereas E2 in combination with AR agonist Enobosarm downregulated ERGs SERPINA3, ATP6V1B1, TFF1 and PR regulated gene RANK. Only GREB1 and CLIC6 were upregulated by E2 and downregulated by the combination of E2 with Enobosarm. DHT + E2 exhibited no significant difference in expression of ER, AR or PR genes examined, compared with the effect of E2 alone. A negative trend was observed between ER protein levels and MD increase in PDEs. Since ER protein gets degraded upon hormone activation, this observation supports active ER signalling in the promotion of MD. Conclusions: Collectively, these findings supports the utility of single-sided Portable NMR for the measurement of MD in explanted human mammary tissue to detect MD change in response to exogenous hormone treatments. A mechanism through which activation of AR may reduce MD as a potential mechanism for reducing breast cancer risk associated to high MD is discussed.

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Section: Introductionmentioning

confidence: 94%

Portable NMR for the investigation of models of mammographic density ex vivo: Androgens antagonise the promotional effect of oestrogen

Huang,

Reye,

Momot

et al. 2023

BSI

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“…One such technique is single-sided portable NMR (pNMR). We have previously demonstrated the ability of pNMR to quantify MD both in excised breast tissue samples in vitro 12,[32][33][34][35][36] and, more recently, in a healthy volunteer trial in vivo. 37 These studies have investigated several MR measurement approaches including transverse relaxation, 33,[35][36][37] diffusion, 34,35 and longitudinal relaxation.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously demonstrated the ability of pNMR to quantify MD both in excised breast tissue samples in vitro 12,[32][33][34][35][36] and, more recently, in a healthy volunteer trial in vivo. 37 These studies have investigated several MR measurement approaches including transverse relaxation, 33,[35][36][37] diffusion, 34,35 and longitudinal relaxation. 32,35 In addition to breast imaging, the utility of pNMR and MRI for the measurement of compositional, structural, and functional characteristics of biological tissues has been demonstrated in a range of other prospective applications including the nervous system, [38][39][40][41] skin, [42][43][44][45][46] cardiovascular and respiratory system, 47-49 musculoskeletal system, [50][51][52][53][54][55][56] and other internal organs.…”

Section: Introductionmentioning

confidence: 99%

“…37 These studies have investigated several MR measurement approaches including transverse relaxation, 33,[35][36][37] diffusion, 34,35 and longitudinal relaxation. 32,35 In addition to breast imaging, the utility of pNMR and MRI for the measurement of compositional, structural, and functional characteristics of biological tissues has been demonstrated in a range of other prospective applications including the nervous system, [38][39][40][41] skin, [42][43][44][45][46] cardiovascular and respiratory system, 47-49 musculoskeletal system, [50][51][52][53][54][55][56] and other internal organs. [57][58][59][60] These studies have covered the measurements both in vitro 41,50,[52][53][54]56 and in vivo in test animals 38,51,58 and in humans.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative measurement of mammographic density in breast‐tissue explants using portable NMR: Precision and accuracy

Foongkajornkiat,

Sokolowski,

Stephenson

et al. 2024

Magnetic Resonance in Med

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PurposeSingle‐sided portable NMR (pNMR) has previously been demonstrated to be suitable for quantification of mammographic density (MD) in excised breast tissue samples. Here we investigate the precision and accuracy of pNMR measurements of MD ex vivo as compared with the gold standards.MethodsForty‐five breast‐tissue explants from 9 prophylactic mastectomy patients were measured. The relative tissue water content was taken as the MD‐equivalent quantity. In each sample, the water content was measured using some combination of three pNMR techniques (apparent T2, diffusion, and T1 measurements) and two gold‐standard techniques (computed microtomography [μCT] and hematoxylin and eosin [H&E] histology). Pairwise correlation plots and Bland–Altman analysis were used to quantify the degree of agreement between pNMR techniques and the gold standards.ResultsRelative water content measured from both apparent T2 relaxation spectra, and diffusion decays exhibited strong correlation with the H&E and μCT results. Bland–Altman analysis yielded average bias values of −0.4, −2.6, 2.6, and 2.8 water percentage points (pp) and 95% confidence intervals of 13.1, 7.5, 11.2, and 11.8 pp for the H&E – T2, μCT – T2, H&E – diffusion, and μCT – diffusion comparison pairs, respectively. T1‐based measurements were found to be less reliable, with the Bland–Altman confidence intervals of 27.7 and 33.0 pp when compared with H&E and μCT, respectively.ConclusionApparent T2‐based and diffusion‐based pNMR measurements enable quantification of MD in breast‐tissue explants with the precision of approximately 10 pp and accuracy of approximately 3 pp or better, making pNMR a promising measurement modality for radiation‐free quantification of MD.

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Portable NMR for quantification of breast density in vivo: Proof-of-concept measurements and comparison with quantitative MRI

Parry

Blick

Foongkajornkiat

et al. 2022

Magnetic Resonance Imaging

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Sensing mammographic density using single-sided portable Nuclear Magnetic Resonance

Cited by 3 publications

References 30 publications

Portable NMR for the investigation of models of mammographic density ex vivo: Androgens antagonise the promotional effect of oestrogen

Portable NMR for the investigation of models of mammographic density ex vivo: Androgens antagonise the promotional effect of oestrogen

Quantitative measurement of mammographic density in breast‐tissue explants using portable NMR: Precision and accuracy

Portable NMR for quantification of breast density in vivo: Proof-of-concept measurements and comparison with quantitative MRI

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