Sensing neuroactive agents in Hawaiian plants

Rechnitz, G. A.; Coon, David R.; Babb, Christopher W.; Ogunseitan, Adeboye; Lee, Amy

doi:10.1016/s0003-2670(96)00424-2

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…This sensor was shown to respond to the antitubercular drug pyrazinamide and several local anesthetics. It was also possible to extract nerves from these legs and use them for the detection of neuroactive plant agents (Rechnitz et al 1997). Both agonists and antagonists can be detected.…”

Section: Biosensors Based On Animal Tissues and Membrane Constituentsmentioning

confidence: 99%

Biosensors: new approaches in drug discovery

Keusgen

2002

Naturwissenschaften

120

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The development of biosensors for analytical purposes has attracted a great deal of attention in recent years. A biosensor is defined as an analytical device consisting of a biological component (e.g., enzyme, antibody, entire cell, DNA) and a physical transducer (e.g., electrode, optical device). Biosensors are mostly designed for routine analysis, such as clinical diagnosis, quality control of food, in-process control of fermentations, and in environmental analysis. Many of these sensors are also suitable for screening purposes in order to find new drugs. Such systems should yield information either about compounds with known bioactivity or about the bioactivity of samples with known or unknown chemical composition. Biosensors intended for the latter purpose are essentially based on whole cells carrying receptors and ion channels at their surfaces. Miniaturization of structures, primarily based on silicon, allows integration of many sensors into arrays, which may be suitable for the screening of natural and chemical products as well as combinatorial libraries. Until now, no commercially available sensors of this kind exist but they are expected in the near future. Different biosensors, based on enzymes, antibodies, cells, artificial membranes and entire animal tissues, which can be used in drug discovery and may lead to efficient screening systems in the future, are described in this review.

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Section: Biosensors Based On Animal Tissues and Membrane Constituentsmentioning

confidence: 99%

Biosensors: new approaches in drug discovery

Keusgen

2002

Naturwissenschaften

120

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“…Such systems are very sensitive to compounds that have their effect by interfering with the conduction ability of the nerve tissue. Neuronal biosensors using electrochemical or biomagnetic transducers have been developed for a wide range of analytes, including neural activity modulatory drugs such as toxins, agonists, and anesthetics. , Neuroreceptors, either cloned and expressed in Xenopus oocytes or as intact ion channels of crustacean nerves, have been employed as molecular recognition elements. Thus far, the experimental arrangements have been limited to flow-based systems with constrained contact between the analyte and the nerve tissue.…”

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confidence: 99%

Neuronal Biosensors Using Liposomal Delivery of Local Anesthetics

1997

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The use of liposomal drug incorporation significantly improves the sensitivity of neuronal sensors to poorly soluble drugs and extends the applicability of such sensors to previously undetectable compounds. Data presented support physiological models for liposome−neuron interactions and are consistent with the channel-blocking actions of local anesthetics. We have incorporated the anesthetics benzocaine, bupivacaine, and tetracaine into liposomes, generating dose−response curves. Liposomes are shown to extend the range of the biosensor and improve its sensitivity.

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