Sensing of bacterial cell viability using nanostructured bioelectrochemical system: rGO-hyperbranched chitosan nanocomposite as a novel microbial sensor platform

Sedki, Mohammed; Hassan, Rabeay Y. A.; Hefnawy, Amr; El‐Sherbiny, Ibrahim M.

doi:10.1016/j.snb.2017.05.163

Cited by 32 publications

(19 citation statements)

References 53 publications

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“…Then the surface of the electrode was removed and dried (at room temperature, for 2 h). The electrodes were thoroughly washed twice with phosphate buffer before being dried again and sputter‐coated with gold ready for morphological characterization using scanning electron microscopy (SEM, JEOL, JXA‐840A) at an accelerating applied potential of 15 keV …”

Section: Methodsmentioning

confidence: 99%

“…The electrodes were thoroughly washed twice with phosphate buffer before being dried again and sputter-coated with gold ready for morphological characterization using scanning electron microscopy (SEM, JEOL, JXA-840A) at an accelerating applied potential of 15 keV. 3,22 Studying the limiting factors of photosynthetically evolved oxygen Dissolved oxygen (DO) was observed during three different algal growth conditions [ Fig. 1(a)].…”

Section: Morphological Characterization Of the Anode-formed Algal Biomentioning

confidence: 99%

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Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems

Mahmoud

Abdo

Samhan

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Microalgae have attracted worldwide interest resulting from their extensive applications in renewable energy and biomass production. However, in algal fuel cells, photosynthetically evolved oxygen hinders the use of algal biofilms formed at the anode surface. Here, nanostructured bio‐electrochemical systems have been designed to explore the algal bio‐electrochemistry at different illumination and growth conditions. RESULTS Three algal strains were screened for their exoelectrogenic activity and the possibility of direct electron transfer to the chemically modified surfaces. After adjusting light and dark conditions and medium compositions, oxygen production within the fuel cells was regulated. At the anode surface, the obtained bioelectrochemical responses and the morphological characterizations suggested that Oscillatoria agardhii has the potential to serve as electron donor and the nanostructured surface is the final electron acceptor. To that end, dual‐chamber algal fuel cells were constructed and glucose (10 g L–1) was used as carbon source. CONCLUSION Power density of 26.8 mW m–2 was produced using the biofilm formed by O. agardhii. Eventually, the consumption of organic waste was monitored, whereas the chemical oxygen demand removal reached 82%. © 2019 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Morphological Characterization Of the Anode-formed Algal Biomentioning

confidence: 99%

Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems

Mahmoud

Abdo

Samhan

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

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“…In the last 10 years, enormousa dvances in nanomaterials and sensinga pproaches, such as the use of graphene, [22,23] CNTs, [24,25] immunomagnetic nanoparticles or beads, [26] screen printed electrodes (SPEs), [27] paper-based [24] and inkjet-printed platforms, [28] as well as interdigitated array microelectrodes [29] have been achieved to improve the specificity and sensitivity of biosensors. [30] Herein, only electrochemically relevant materials will be discussed to illustrate how improve the sensitivity, specificity and automation of the pathogen biosensors can be improved.…”

Section: Methodsmentioning

confidence: 99%

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Kuss

Amin

Compton

2018

Chemistry An Asian Journal

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Bacterial infections represent one of the leading causes of mortality worldwide, nevertheless the design and development of rapid, cost-efficient and reliable detection methods for pathogens remains challenging. In recent years, electrochemical sensing methods have gained increasing attention for the detection of pathogenic bacteria, due to their increasingly competitive sensitivity. However, combining sensitivity with cost efficiency, high selectivity and a facile working procedure in a portable device is difficult. The presented review provides a summary of biosensing strategies for bacteria, published since 2015, by covering significant achievements towards custom-designed portable point-of-care devices. Herein, the direct chemical recognition of bacteria via enzyme activity or secretion products, as well as their detection at various electrode surfaces and materials, such as nanomaterials, indium tin oxide or paper-based immunosensors, is discussed. Furthermore, newly established hyphenated sensing principles, incorporated into lab-on-a-chip and microfluidic devices, are presented and remaining technical challenges and limitations are considered.

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“…Sedki’s group developed a nanocomposite of reduced graphene oxide (RGO) with hyperbranched chitosan to develop sensor platform for monitoring cell viability [265]. The target bacterium was P. aeruginosa and its viability was tested in the presence of various antibiotics, such as ciprofloxacin, simvastatin, and kanamycin.…”

Section: Organic Nanomaterials Applications For Healthcare Biosensingmentioning

confidence: 99%

Nanomaterials for Healthcare Biosensing Applications

Pirzada

Altıntaş

2019

Sensors

188

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In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field. Nanomaterials introduce versatility to the sensing platforms and may even allow mobility between different detection mechanisms. The prospect of a combination of different nanomaterials allows an exploitation of their synergistic additive and novel properties for sensor development. This paper covers more than 290 research works since 2015, elaborating the diverse roles played by various nanomaterials in the biosensing field. Hence, we provide a comprehensive review of the healthcare sensing applications of nanomaterials, covering carbon allotrope-based, inorganic, and organic nanomaterials. These sensing systems are able to detect a wide variety of clinically relevant molecules, like nucleic acids, viruses, bacteria, cancer antigens, pharmaceuticals and narcotic drugs, toxins, contaminants, as well as entire cells in various sensing media, ranging from buffers to more complex environments such as urine, blood or sputum. Thus, the latest advancements reviewed in this paper hold tremendous potential for the application of nanomaterials in the early screening of diseases and point-of-care testing.

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Sensing of bacterial cell viability using nanostructured bioelectrochemical system: rGO-hyperbranched chitosan nanocomposite as a novel microbial sensor platform

Cited by 32 publications

References 53 publications

Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems

Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Nanomaterials for Healthcare Biosensing Applications

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