Sensing of mercury ions in Porphyra by Copper @ Gold nanoclusters based ratiometric fluorescent aptasensor

Shi, Yongqiang; Li, Wenting; Feng, Xuping; Lin, Lei; Nie, Ping; Shi, Jiyong; Zhang, Xiaobo; He, Yong

doi:10.1016/j.foodchem.2020.128694

Cited by 85 publications

(30 citation statements)

References 37 publications

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“…In these cases, the underlying mechanism is usually ascribed to Förster resonance energy transfer (FRET). A probe based on aptamer-modified Copper @ Gold nanoclusters (apt-Cu@Au NCs) was designed for ratiometric detection of Hg 2+ [ 63 ]. The mercury ions were captured by T-rich nucleic acid single strands on the surface of nanoclusters.…”

Section: Preparation Methods and Sensing Mechanism Of Cu Ncsmentioning

confidence: 99%

“…This on-site detection and monitoring of the target analytes in real samples (e.g., tap, river and pond water) could be achieved easily with the developed cheap smartphone-aided and paper-based kit. Shi et al [ 63 ] developed a ratiometric sensing strategy toward mercury ions by modifying copper nanoclusters @ gold nanoclusters via aptamer (apt-Cu@Au NCs). The changes in the fluorescence of this novel probe is attributed to the FRET strategy, which was achieved through the thymidine–Hg–thymidine (T–Hg–T) structure generated by addition of Hg 2+ into the solution containing apt-Cu@Au NCs.…”

Section: Sensing Applications Based On Cu Ncsmentioning

confidence: 99%

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Synthesis of Copper Nanocluster and Its Application in Pollutant Analysis

et al. 2021

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Copper nanoclusters (Cu NCs) with their inherent optical and chemical advantages have gained increasing attention as a kind of novel material that possesses great potential, primarily in the use of contaminants sensing and bio-imaging. With a focus on environmental safety, this article comprehensively reviews the recent advances of Cu NCs in the application of various contaminants, including pesticide residues, heavy metal ions, sulfide ions and nitroaromatics. The common preparation methods and sensing mechanisms are summarized. The typical high-quality sensing probes based on Cu NCs towards various target contaminants are presented; additionally, the challenges and future perspectives in the development and application of Cu NCs in monitoring and analyzing environmental pollutants are discussed.

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Section: Preparation Methods and Sensing Mechanism Of Cu Ncsmentioning

confidence: 99%

Section: Sensing Applications Based On Cu Ncsmentioning

confidence: 99%

Synthesis of Copper Nanocluster and Its Application in Pollutant Analysis

et al. 2021

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“…The fluorescence intensity and Hg 2+ concentration had good linear correlation in the range of 2–160 n and detection limit was 0.2 nM. Shi et al [ 101 ] developed a FRET aptasensor based on T-Hg 2+ -T double-stranded structure. The aptamer-modified Copper @ Gold nanoclusters (apt-Cu@Au NCs) aggregated in the solution in the presence of Hg 2+ , resulting in change of fluorescence intensity.…”

Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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Heavy metal pollution has become more and more serious with industrial development and resource exploitation. Because heavy metal ions are difficult to be biodegraded, they accumulate in the human body and cause serious threat to human health. However, the conventional methods to detect heavy metal ions are more strictly to the requirements by detection equipment, sample pretreatment, experimental environment, etc. Aptasensor has the advantages of strong specificity, high sensitivity and simple preparation to detect small molecules, which provides a new direction platform in the detection of heavy metal ions. This paper reviews the selection of aptamers as target for heavy metal ions since the 21th century and aptasensors application for detection of heavy metal ions that were reported in the past five years. Firstly, the selection methods for aptamers with high specificity and high affinity are introduced. Construction methods and research progress on sensor based aptamers as recognition element are also introduced systematically. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.

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“…Unlike antibodies, aptamers are relatively stable under extreme temperature conditions for a short time (14). Therefore, several platforms of aptamer sensors, such as fluorescence (15), colorimetry (16), and electrochemistry (17), have been developed for the detection of pesticides. To improve the stability and sensitivity of aptamer-based assays, nanomaterials (one of the most interesting sensing materials) are bioconjugated with aptamers for selective and sensitive detection of analytes.…”

Section: Introductionmentioning

confidence: 99%

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

Zhai

Liu

et al. 2022

Front. Nutr.

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Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe3O4 was functionalized with a complementary strand of DNA (C-DNA) to form Fe3O4 probes (Fe3O4-C-DNA). Subsequently, the CBZ and the Fe3O4 probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe3O4). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe3O4 biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products.

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Sensing of mercury ions in Porphyra by Copper @ Gold nanoclusters based ratiometric fluorescent aptasensor

Cited by 85 publications

References 37 publications

Synthesis of Copper Nanocluster and Its Application in Pollutant Analysis

Synthesis of Copper Nanocluster and Its Application in Pollutant Analysis

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

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