Sensing of survivin mRNA in malignant astrocytes using graphene oxide nanocarrier-supported oligonucleotide molecular beacons

Stobiecka, Magdalena; Dworakowska, Beata; Jakieła, Sławomir; Łukasiak, Agnieszka; Chałupa, Agata; Zembrzycki, Krzysztof

doi:10.1016/j.snb.2016.04.176

Cited by 63 publications

(51 citation statements)

References 52 publications

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“…However, in our study, the plant phenotype on the GO/MS medium was similar overall to that on the MS medium, and GO was confirmed to be noncytotoxic to the plant. Stobiecka et al, revealed that GO was aggregated at the higher concentration than 80 µg/mL in a phosphate-buffered saline, and the stacking interactions of GO layer was accelerated at the higher concentration of >17 µg/mL in a phosphate buffer [24]. This means a possibility that the small amounts of GO used in this work might be minimized for the aggregation in the agar plate and the GO solution.…”

Section: Direct Influence Of Graphene Oxide On Watermelonmentioning

confidence: 78%

Graphene Oxide-Assisted Promotion of Plant Growth and Stability

et al. 2020

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The control and promotion of plant and crop growth are important challenges globally. In this study, we have developed a nanomaterial-assisted bionic strategy for accelerating plant growth. Although nanomaterials have been shown to be toxic to plants, we demonstrate herein that graphene oxide can be used as a regulator tool for enhancing plant growth and stability. Graphene oxide was added to the growth medium of Arabidopsis thaliana L. as well as injected into the stem of the watermelon plant. We showed that with an appropriate amount provided, graphene oxide had a positive effect on plant growth in terms of increasing the length of roots, the area of leaves, the number of leaves, and the formation of flower buds. In addition, graphene oxide affected the watermelon ripeness, increasing the perimeter and sugar content of the fruit. We believe that graphene oxide may be used as a strategy for enabling the acceleration of both plant growth and the fruit ripening process.

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Section: Direct Influence Of Graphene Oxide On Watermelonmentioning

confidence: 78%

Graphene Oxide-Assisted Promotion of Plant Growth and Stability

et al. 2020

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“…It was also shown that polycystin-1 co-localizes with E-cadherin at the cell-cell contacts, accelerating the recruitment of intracellular E-cadherin to reforming junctions [40]. These exosome proteins could be proposed as targets for the detection of a cancer biomarker [41,42,43]. …”

Section: Discussionmentioning

confidence: 99%

Exosome Proteome of U-87MG Glioblastoma Cells

Chun

Ahn

Yeom³

et al. 2016

Biology

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Exosomes are small membrane vesicles between 30 and 100 nm in diameter secreted by many cell types, and are associated with a wide range of physiological and/or pathological processes. Exosomes containing proteins, lipids, mRNA, and microRNA contribute to cell-to-cell communication and cell-to-environment regulation, however, their biological functions are not yet fully understood. In this report, exosomes in the glioblastoma cell line, U-87MG, were isolated and the proteome was investigated. In addition, exosome proteome changes in U-87MG cells exposed to a low temperature were investigated to elucidate whether the exosome proteome could respond to an external stimulus. Cell culture medium was collected, and exosomes were isolated by continuous centrifugation eliminating cell debris, nucleic acids, and other particles. The morphology of exosomes was observed by cryo-tunneling electron microscopy. According to 2-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, certain proteins including collagen type VI alpha 1, putative RNA-binding protein 15B chain A, substrate induced remodeling of the active site regulates HTRA1, coatomer protein complex-subunit beta 2, myosin-heavy chain 1, and keratin-type I cytoskeletal 9 showed differences between the control proteome and the low temperature-exposed proteome.

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“…Among the many potential predictive biomarkers is the protein survivin which is an inhibitor of apoptosis and plays crucial role in the regulation of cell division and cell cycle control. Survivin is strongly expressed in malignant tumors [12][13][14]. Survivin has been tested in both the in vivo and in vitro setting, however, information regarding it use in human cancers has been challenged.…”

Section: Clinical Utility Of Biomarkers In Cancermentioning

confidence: 99%

“…Survivin has been tested in both the in vivo and in vitro setting, however, information regarding it use in human cancers has been challenged. Using nanotechnology the determination of survivin will be easier and its use in cancer research and cancer treatment will meet the requirements for real time [12]. The oligonucleotide molecular beacons encoded for survivin mRNA have been proposed for the indirect detection of survivin expression [15].…”

Section: Clinical Utility Of Biomarkers In Cancermentioning

confidence: 99%

Some aspects of cancer biomarkers and their clinical application in solid tumors – revisited

Isaac¹,

Elayoubi²,

A³

et al. 2017

J Cancer Res Ther.

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Cancer biomarkers can be used for a variety of purposes related to screening, prediction, stratification, detection, diagnosis, prognosis, treatment design, and monitoring of a therapeutic response. One of the most important characteristics of a given biomarker includes ease of collection allowing for a non-invasive approach and frequent sampling. Such samples may be obtained from serum or plasma, sputum, bronchoalveolar lavage, saliva, nipple discharge, pleural, or peritoneal effusions. Validation of different biomarkers is considered a mandatory method for useful evaluation. In this review, we highlight the clinical applicability of some cancer biomarkers, as well as future approaches for their development and collection, which may help guide clinicians and researchers. The role of liquid biopsies will also be summarized. Further studies using liquid biopsies are needed to elucidate the significance of various sources of biomarkers suitable for clinical application.

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Sensing of survivin mRNA in malignant astrocytes using graphene oxide nanocarrier-supported oligonucleotide molecular beacons

Cited by 63 publications

References 52 publications

Graphene Oxide-Assisted Promotion of Plant Growth and Stability

Graphene Oxide-Assisted Promotion of Plant Growth and Stability

Exosome Proteome of U-87MG Glioblastoma Cells

Some aspects of cancer biomarkers and their clinical application in solid tumors – revisited

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