2006
DOI: 10.1063/1.2180868
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Sensing protein molecules using nanofabricated pores

Abstract: We report the detection of protein molecules with nanofabricated pores using the resistive pulse sensing method. A 20-nm-thick silicon nitride membrane with a nanofabricated pore measuring about 55nm in diameter separated an electrolyte cell into two compartments. Current spike trains were observed when bovine serum albumin (BSA) was added to the negatively biased compartment. The magnitude of the spikes corresponded to particles 7–9nm in diameter (the size of a BSA molecule) passing through the pore. This sug… Show more

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Cited by 171 publications
(171 citation statements)
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“…The nanochannel fabrication process is based on partial etching of a sacrificial layer [25]. The fabrication process only requires established micromachining techniques and does not require any nanolithography.…”
Section: Microfabrication Process Of Nanochannelsmentioning
confidence: 99%
See 1 more Smart Citation
“…The nanochannel fabrication process is based on partial etching of a sacrificial layer [25]. The fabrication process only requires established micromachining techniques and does not require any nanolithography.…”
Section: Microfabrication Process Of Nanochannelsmentioning
confidence: 99%
“…This was only possible because of the recent advances in the innovative development of cost-effective fabrication methods of nanochannels [15,25,[34][35][36]. The filling rate decreased with diminishing channel depth (Fig.…”
Section: Washburn Kinetics In Channels With Different Depthmentioning
confidence: 99%
“…For example, nucleic acid and protein analysis has perhaps been the most popular 1,14,15 . The predominant method used for the detection of a translocation event (i.e.…”
mentioning
confidence: 99%
“…Therefore, nanopore-based techniques are powerful meth-ods for sensing single molecules. Nanopore technique has been shown to be a versatile tool for studying size, charge, or folding state of proteins [14,21,[24][25][26] according to the modulation of ionic current caused by their translocation. Solid-state nanopores have prominent advantages of characterizing single molecules including the versatility of pore dimensions, robustness, longer lifetime, and high chemical resistance to denaturing agents [10,[27][28][29][30][31][32][33][34].…”
Section: Introductionmentioning
confidence: 99%
“…Nanopores, both biological nanopore [1][2][3][4][5] and solid-state nanopore [6][7][8], have been developed to be very promising candidates for biological detection at single molecule level, such as DNA [7,[9][10][11], RNA [12,13] and proteins [14][15][16][17][18][19][20][21][22][23]. The detection of single molecules is realized through monitoring the temporary ionic current blockage during single molecule translocation through nanopore which is driven by external electric field.…”
Section: Introductionmentioning
confidence: 99%