Sensing Reversible Protein–Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors

Am, Münzer; W, Seo; Gj, Morgan; Zp, Michael; Zhao, Yong; Melzer, K.; Scarpa, Giuseppe

doi:10.1021/jp503670a

Cited by 34 publications

(23 citation statements)

References 48 publications

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“…For example, the various preparations steps during growth or during the fabrication of devices may introduce chemical groups (such as oxygen-containing functionalities) that may affect the charge density on the graphene surface. Similar observations have also been made on carbon nanotubes (CNTs) 17 18 . The isoelectric point (IEP or pI)/point-of-zero charge (pzc) provides a measure of the acid-base properties of the ionizable groups and in turn the surface charge behavior as a function of solution pH 19 20 .…”

supporting

confidence: 78%

Tuning the isoelectric point of graphene by electrochemical functionalization

Zuccaro

Krieg

Desideri

et al. 2015

Sci Rep

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The ability to control the charge-potential landscape at solid-liquid interfaces is pivotal to engineer novel devices for applications in sensing, catalysis and energy conversion. The isoelectric point (pI)/point of zero charge (pzc) of graphene plays a key role in a number of physico-chemical phenomena occurring at the graphene-liquid interface. Supported by theory, we present here a methodology to identify the pI/pzc of (functionalized) graphene, which also allows for estimating the nature and extent of ion adsorption. The pI of bare graphene (as-prepared, chemical vapor deposition (CVD)-grown) is found to be less than 3.3, which we can continuously modify up to 7.5 by non-covalent electrochemical attachment of aromatic amino groups, preserving the favorable electronic properties of graphene throughout. Modelling all the observed results with detailed theory, we also show that specific adsorption of ions and the substrate play only an ancillary role in our capability to tune the pI of graphene.

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supporting

confidence: 78%

Tuning the isoelectric point of graphene by electrochemical functionalization

Zuccaro

Krieg

Desideri

et al. 2015

Sci Rep

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“…Moreover, FET biosensors are workable to small amounts of biomolecules in physiological solution, suitable to detect human cancer and disease. [ 12‐15 ] As shown in Figure 1, FET biosensors have been applied to biomolecular monitor, including nucleic acid sensing, [ 16‐20 ] protein sensing, [ 21‐25 ] as well as cell or bacterium. [ 26‐30 ]…”

Section: Introductionmentioning

confidence: 99%

“…Schematic application of FET biosensor in nucleic acid, protein, cell and bacterium. Reproduced with permission from Ref [19] (Copyright 2019, American Chemical Society), Ref [20] (Copyright 2015, American Chemical Society), Ref [24] (Copyright 2014, American Chemical Society), Ref [25] (Copyright 2019, American Chemical Society), Ref [29] (Copyright 2016, American Chemical Society), Ref [30] (Copyright 2019, American Chemical Society).…”

Section: Introductionmentioning

confidence: 99%

Overcome Debye Length Limitations for Biomolecule Sensing Based on Field Effective Transistors^†

et al. 2021

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Biosensors based on field effective transistor (FET) have aroused tremendous attention in the past few years owning to their huge application in drug discovery, disease diagnosis and environmental monitoring. The FET biosensors possess small volume, high sensitivity at ultra-low concentration, considerable mechanical strength, as well as excellent stability in solution, which plays a vital role in the point of care testing (POCT) systems. Recent advances have summarized some progress involved in the improvement of morphology and structure of channel materials, the functionalization of organic molecule, the influence of device operation and sensing environment on the detecting performance. However, for FET biosensors, the charge screening phenomena were inevitable in the solution, which seriously degrade the device performance. In this article, we summarize recent advances to overcome debye length limitations for biomolecule sensing based on FET. We will firstly describe the charge screening mechanism, then focous on the strategy to overcome charge screening, including synthesizing special channel materials with crumpled morphology, designing aptamer binding mode, and modulating device measurement. Finally, we discuss the major challenges and perspectives about overcoming debye length limitations of FET biosensors. These summaries provide further insights to realize real-time, lable-free, high-sensitivity FET sensors for medical healthcare. Zhi Zheng (left) received his Ph.D. degree in Prof. Tianyou Zhai's group at the School of Materials Science and Engineering, HUST. He is currently an associate professor of Faculty of Materials Science and Chemistry, China University of Geosciences (Wuhan). His scientific research concentrates on the preparation of large-area two-dimensional materials for field effective transistors biosensors. Hongyuan Zhang (middle) obtained his bachelor degree in School of Resources and Civil Engineering in 2017 at Northeastern University. He is currently pursuing his Ph.D. degree under the supervision of Prof. Fan Xia in Faculty of Materials Science and Chemistry at China University of Geosciences (Wuhan). His current research interests mainly focused on the biosensors based on field effect transistor. Fan Xia (right) studied physical chemistry at the Institute of Chemistry, Chinese Academy of Sciences (ICCAS), where he received Ph.D. degree in 2008 under the supervision of Prof. Lei Jiang. He then worked as a postdoctoral fellow in Prof. Alan J. Heeger's group at the University of California, Santa Barbara. He joined HUST as part of the 1000 Young Talents Program in 2012 and became professor at HUST. He is currently a professor and Dean of Faculty of Materials Science and Chemistry, China University of Geosciences (Wuhan). His scientific interest is focused on bioanalytical chemistry.

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“…A straightforward way to achieve more specific analyte sensing using the CNT sensor array would include to control the nature of the integrated CNTs by either sorting them based on their electronic properties or by performing a functionalization step prior to integration by means of DEP, as has been described in Hennrich et al [18] A second route is to functionalize the CNT surface after DEP integration on the CMOS system. [35,36] We have demonstrated the potential of using a DEP-based protocol for the parallel deposition of CNTs on electrode pairs of a CMOS system. We developed an interdigitated floating-electrode configuration to fabricate 1024 independent CNT devices on a CMOS array in parallel.…”

mentioning

confidence: 99%

Dielectrophoresis‐Assisted Integration of 1024 Carbon Nanotube Sensors into a CMOS Microsystem

Seichepine¹,

Rothe

Dudina

et al. 2017

Advanced Materials

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Carbon-nanotube (CNT)-based sensors offer the potential to detect single-molecule events and picomolar analyte concentrations. An important step toward applications of such nanosensors is their integration in large arrays. The availability of large arrays would enable multiplexed and parallel sensing, and the simultaneously obtained sensor signals would facilitate statistical analysis. A reliable method to fabricate an array of 1024 CNT-based sensors on a fully processed complementary-metal-oxide-semiconductor microsystem is presented. A high-yield process for the deposition of CNTs from a suspension by means of liquid-coupled floating-electrode dielectrophoresis (DEP), which yielded 80% of the sensor devices featuring between one and five CNTs, is developed. The mechanism of floating-electrode DEP on full arrays and individual devices to understand its self-limiting behavior is studied. The resistance distributions across the array of CNT devices with respect to different DEP parameters are characterized. The CNT devices are then operated as liquid-gated CNT field-effect-transistors (LG-CNTFET) in liquid environment. Current dependency to the gate voltage of up to two orders of magnitude is recorded. Finally, the sensors are validated by studying the pH dependency of the LG-CNTFET conductance and it is demonstrated that 73% of the CNT sensors of a given microsystem show a resistance decrease upon increasing the pH value.The potential of carbon nanotubes (CNTs) as electronic sensors in liquid environments has been extensively demonstrated. Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts electronic environment and the nanotube's high surface/volume ratio render them particularly suited for sensing applications in liquid phases. Furthermore, through functionalization of the CNTs, a specificity for numerous analytes can be achieved. [1][2][3][4][5] Highly sensitive CNT nanosensors have been demonstrated in liquid-gate CNT field-effecttransistor (LG-CNTFET) configurations, where the carrier density of the CNT gate was controlled via a potential applied to the surrounding electrolyte.[6] By using LG-CNTFET sensors consisting of only a single or a small number of individual CNTs as sensing material, only few molecules are required to generate detectable signals. In the case of DNA hybridization, even the detection of a single molecule is possible. [7,8] Despite their application potential in point-of-care diagnosis and label-free sensing, the widespread use of CNT nanosensor technologies is still hampered by technological hurdles. First of all, a route to fabricating large numbers of devices with reproducible characteristics has to be developed. Additionally, the high sensitivity of CNT sensors entails the presence of baseline fluctuations due to unspecific and random adsorption events, which are likely to create detection errors.[9] Finally, the limited adsorption area of single-CNT nanosensors leads to slow response times for low concentrations.[10]A possibility to overcome some o...

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Sensing Reversible Protein–Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors

Cited by 34 publications

References 48 publications

Tuning the isoelectric point of graphene by electrochemical functionalization

Tuning the isoelectric point of graphene by electrochemical functionalization

Overcome Debye Length Limitations for Biomolecule Sensing Based on Field Effective Transistors^†

Dielectrophoresis‐Assisted Integration of 1024 Carbon Nanotube Sensors into a CMOS Microsystem

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Sensing Reversible Protein–Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors

Cited by 34 publications

References 48 publications

Tuning the isoelectric point of graphene by electrochemical functionalization

Tuning the isoelectric point of graphene by electrochemical functionalization

Overcome Debye Length Limitations for Biomolecule Sensing Based on Field Effective Transistors†

Dielectrophoresis‐Assisted Integration of 1024 Carbon Nanotube Sensors into a CMOS Microsystem

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Product

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Overcome Debye Length Limitations for Biomolecule Sensing Based on Field Effective Transistors^†