Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography

Chen, Guang; Li, Jianjun; Liu, Mengge; Li, Guoliang; Sun, Zhi‐Wei; Zhang, Shijuan; Song, Cuihua; Wang, Hua; Suo, Yourui; You, Jing

doi:10.1016/j.chroma.2014.05.061

Cited by 18 publications

(8 citation statements)

References 44 publications

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“…Primarily, large amounts of pharmaceutical matrix components undoubtedly exert severe matrix interferences, resulting in a decrease of method specificity and sensitivity. Another complication is that, drug substance samples cannot experience highly direct concentration in case that the pharmaceutical matrix components will also be concentrated together with the amines, which brings about more matrix interferences . Moreover, other tedious preconcentration techniques, such as SPME and solvent bar microextraction , readily cause the loss of aliphatic amines even at ambient temperature.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, other tedious preconcentration techniques, such as SPME and solvent bar microextraction , readily cause the loss of aliphatic amines even at ambient temperature. This may account for the fact that some methods with complex enrichment process provided low recoveries and repeatability for aliphatic amines .…”

Section: Introductionmentioning

confidence: 99%

“…Over the years, many efforts have been made to quantitate aliphatic amines at a low level from complex matrixes using various derivatization techniques. This has facilitated the appearance of many derivatization reagents, such as o ‐phthalaldehyde and 9‐fluorenylmethyl chloroformate , dansyl chloride , naphthalene‐2,3‐dicarboxaldehyde , pentafluorobenzoyl chloride , 2,3,4,5,6‐pentafluorobenzaldehyde , and other self‐prepared derivatization reagents . However, for those reported derivatization methods, a potential weakness is that the excessive unreacted reagents are usually difficult to separate from the derivatization products due to their similar properties, therefore, application of tedious and time‐consuming pretreatments or dedicated separation techniques is always required .…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of eight short‐chain aliphatic amines in drug substances by HPLC with diode array detection after derivatization with halonitrobenzenes

Han

Hou

et al. 2017

J of Separation Science

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Short-chain aliphatic amines are a class of hazardous impurities in drug substances. A simple method, involving derivatization followed by high-performance liquid chromatography with diode array detection, has been developed for residue determination of eight aliphatic amines simultaneously in drug substances. Different halonitrobenzenes derivatization reagents were systematically compared. As a result, 1-fluoro-2-nitro-4-(trifluoromethyl)benzene was selected since the derivatization effectively shifted the absorption wavelength to the visible region (400-450 nm), where most drug substances, impurities and even the derivatization reagent absorb very weakly. Due to the redshift effect, interference was minimized and adequately low limits of quantitation were reached (0.24-0.80 nmol/mL). Moreover, the derivatization reaction was readily carried out in dimethyl sulfoxide at room temperature for 1 h using N,N-diisopropylethylamine as catalyst to achieve the highest yield. Without any pre-treatment, the derivatives were analyzed by high-performance liquid chromatography with diode array detection. The high stability of the derivatives within 24 h at room temperature (RSD<1.04%) further facilitated the simultaneous preparation and consecutive analysis of quantities of samples. Finally, the proposed method was successfully applied for residue determination of eight aliphatic amines simultaneously in eight drug substance samples. This study could be helpful for the routine analysis and residue control of aliphatic amines in drug substances.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous determination of eight short‐chain aliphatic amines in drug substances by HPLC with diode array detection after derivatization with halonitrobenzenes

Han

Hou

et al. 2017

J of Separation Science

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“…[22][23][24] For species with poor ionization, fluorescent detection allows "universal" discovery of tagged species. Additionally, a comparison of the fluorescent trace to MS/MS-based search results quickly discriminates between real and false hits because only labeled species have a fluorescent signal.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to the three components, there is also a mixture of antibody-drugs and conjugation sites. The antibody-drug ratio (DAR) and sites play important roles in ADC pharmacokinetics profile, efficacy and safety; [23][24][25] therefore identification of conjugation DAR and sites is essential to understanding ADC properties.…”

Section: Protein Conjugationsmentioning

confidence: 99%

Discovery and elucidation of unknown protein modifications : brominated coumarin azide as a probe for alkynes

Yang¹

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Novel Flow‐through Potentiometric System for Dimethyamine Assessment Using New Ion Exchangers Doped‐polymeric Membrane Sensors

2018

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New simple potentiometric sensing systems are described for the sensitive and selective monitoring of dimethylamine (DMA). These systems are based on using polymeric membranes doped in some ion exchangers such as dimethylamine/phosphomolybdate [(CH3)2NH3]22+[MP]2− (Sensor I), dimethylamine/tetraphenylborate [(CH3)2NH3]+[TPB]− (Sensor II) and dimethylamine/phosphotungstate [(CH3)2NH3]+[TP]− (sensor III). All the sensing systems are incorporated in poly(vinyl chloride) matrix plasticized with o‐NPOE. In a Trizma buffer solution of pH 7.1, the sensors exhibit a stable, fast and linear response for 5.0×10−6–1.0×10−2, 2.5×10−5–1.0×10−2, and 5.0×10−6–1.0×10−2 mol L−1 DMA+ with slopes of 53.2±0.4, 49.4±0.6, and 53.8±0.3 mV decade−1, and detection limits of 2.0×10−6 (0.16 μg mL−1), 1.6×10−5(1.3 μg mL−1), and 1.4×10−6 (0.11 μg mL−1) mol L−1 for sensors (I), (II), and (III), respectively. Potentiometric selectivity measurements of these sensors reveal negligible interferences from most common aliphatic and aromatic amines. Satisfactory results are obtained with an average accuracy of 99.5 % and a mean precision of ±0.5 %. Validation of the proposed assay methods are examined by measuring the limit of detection, linearity range, accuracy, precision, within‐day repeatability, between‐days reproducibility and method ruggedness. Good performance characteristics and applicability of these sensors for DMA assessment in different matrices under static and hydrodynamic modes of measurements are demonstrated. The proposed sensors are also prepared in a tubular form and used as DMA detector in a two‐channel flow injection setup for continuous monitoring. The frequency rate is 27–53 samples h−1. The sensors are satisfactory applied for DMA quantification in both biological matrices and environmental samples with recoveries ranging between 95.0 and 104.0 %.

show abstract

Sensitive, accurate and rapid detection of trace aliphatic amines in environmental samples with ultrasonic-assisted derivatization microextraction using a new fluorescent reagent for high performance liquid chromatography

Cited by 18 publications

References 44 publications

Simultaneous determination of eight short‐chain aliphatic amines in drug substances by HPLC with diode array detection after derivatization with halonitrobenzenes

Simultaneous determination of eight short‐chain aliphatic amines in drug substances by HPLC with diode array detection after derivatization with halonitrobenzenes

Discovery and elucidation of unknown protein modifications : brominated coumarin azide as a probe for alkynes

Novel Flow‐through Potentiometric System for Dimethyamine Assessment Using New Ion Exchangers Doped‐polymeric Membrane Sensors

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