Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice

Fingas, Felix; Volke, Daniela; Hassert, Rayk; Fornefett, Juliane; Funk, Sophie; Baums, Christoph Georg; Hoffmann, Ralf

doi:10.1186/s12866-019-1417-7

Cited by 12 publications

(8 citation statements)

References 32 publications

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“…Experimental infection is associated with IgG seroconversion even at a low dose of 10 4 CFU. This is detectable either by using whole cell extracts as antigen as shown in this study or in the case of R. heylii by using CARLO-1 as antigen as described previously [12]. Additionally, to demonstrating virulence in R. heylii this study describes a colonization model which is suitable for further studies on host-pathogen interaction and prevention measures.…”

Section: Discussionmentioning

confidence: 86%

“…As shown in Fig. 1, the gene encoding the "characteristic antigen for Rodentibacter of laboratory origin 1" (CARLO-1 [12]) was detected but none of the three known pnx genes using DNA of strain R. heylii SF27GVG in contrast to the R. pneumotropicus strain JF4Ni previously characterized in experimental infections [8].…”

Section: Genotyping Of the R Heylii Challenge Strain Sf27gvgmentioning

confidence: 89%

“…Wells were washed three times (PBS, 300 μL) using a Columbus Pro ELISA washer (Tecan), blocked with Superblock (300 μL, 30 min, RT), and stored at 4 °C. ELISA was performed as described before [12]. Cut offs were determined as mean of negative controls (PBS) plus three standard deviations.…”

Section: Antigen Preparation and Elisamentioning

confidence: 99%

“…Experimental infection with 10 8 CFU M. muris 694/11does not induce mortality or high morbidity, confirming that this member of the Pasteurellaceae is of low virulence, at the most. R. heylii and M. muris colonize the upper respiratory tract very efficiently even after low infection doses.MethodsBacterial strains and culture medium R. heylii SF27GVG (pnxI-, pnxII-, pnxIII-) was isolated in 2017 from a mouse in a German research facility[12] and cultured overnight at 37 C on Columbia Blood Agar (Thermo Fischer scientific) or in Brain-Heart-Infusion (BHI; Becton, Dickinson and Company, France) containing 10% inactivated horse serum. M. muris 694/11was cultivated the same way except that BHI did not include horse serum.…”

mentioning

confidence: 99%

“…No amplicon was produced from DNA of R.heylii SF27GVG in contrast to R. pneumotropicus JF4Ni. D: The gene encoding the "characteristic antigen for Rodentibacter of laboratory origin 1" (CARLO-1,[12]) was detected using the same DNA preparation of R. heylii SF27GVG.…”

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confidence: 99%

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A Rodentibacter heylii strain lacking all known RTX toxin genes is highly virulent in C57BL/6 and BALB/c mice in contrast to Muribacter muris

Kähl¹,

Fornefett²,

Fingas³

et al. 2019

Preprint

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Background Rodentibacter (R.) heylii and Muribacter (M.) muris are frequently detected in laboratory rodents. Repeats in toxin (RTX) toxins are considered to be important virulent factors in R. pneumotropicus and R. heylii. As many R. heylii isolates do not carry genes encoding known RTX toxins we hypothesized that these isolates are at the most moderately virulent or even avirulent as M. muris . To test this hypothesis, we evaluated the virulence of R. heylii and M. muris strains negative for all known RTX toxin genes in experimental infections of C57BL/6 and BALB/c mice. Results Experimental intranasal infection with 10 8 colony forming units (CFU) of a pnx I-, pnx II- and pnx III-negative R. heylii strain resulted in 75% and 100% mortality of C57BL/6 and BALB/c mice, respectively. Infections of multiple internal organs such as lung and genito-urinary tract were recorded. Purulent bronchopneumonia was a common finding in lungs of early losses. Application of 10 4 CFU of the same R. heylii strain was neither associated with clinical signs nor dissemination, but with efficient colonization of the upper respiratory tract. Intranasal application of M. muris in different doses ranging from 10 4 to 10 8 CFU did not result in mortality or severe weight loss but efficient colonization and induction of systemic M. muris specific IgG in most animals. Conclusion The current study reveals high virulence of R. heylii strain SF27GVG carrying none of the known RTX toxin genes in wildtype mice. This result questions the validity of estimating virulence in the genus Rodentibacter by profiling of pnx toxin genes. Suitable colonization models for future investigations were established for R. heylii and M. muris . Application of M. muris was associated with a systemic IgG immune response and cultural detection in draining lymph nodes in most animals indicating infection and not sole colonization.

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Section: Discussionmentioning

confidence: 86%

Section: Genotyping Of the R Heylii Challenge Strain Sf27gvgmentioning

confidence: 89%

Section: Antigen Preparation and Elisamentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Rodentibacter heylii strain lacking all known RTX toxin genes is highly virulent in C57BL/6 and BALB/c mice in contrast to Muribacter muris

Kähl¹,

Fornefett²,

Fingas³

et al. 2019

Preprint

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Rodentibacter

Nicklas¹,

Bisgaard²,

Boot³

et al. 2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Ro.den.ti.bac'ter. N.L. pl. n. rodentes rodents, rodens L. pres. part. gnawing; N.L. masc. n. bacter rod; N.L. masc. n. Rodentibacter rod from rodents. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Rodentibacter The genus Rodentibacter was established based on the phenotypic and genotypic characterization of strains, predominantly from rodents. The strains formed a monophyletic group within Pasteurellaceae based on 16S rRNA gene sequence analysis and showed 93.8% or higher similarity, and their unique relationship at genus level within Pasteurellaceae was confirmed by phenotypic analysis. Comparison of a partial region of the rpoB gene sequence showed 86% or higher similarity of all strains. The type species Rodentibacter pneumotropicus was reclassified from [ Pasteurella ] pneumotropica , and the genus further includes seven validly published names of species and two genomospecies. The species were classified based on comparisons of 16S rRNA gene sequences, partial rpoB gene sequences, DNA–DNA renaturation estimated from whole‐genomic sequences, and phenotypes. Rodentibacter heylii includes the former biovar Heyl of [ P .] pneumotropica . Rodentibacter ratti includes taxon 22 of Bisgaard, while Rodentibacter myodis includes taxon 41 of Bisgaard. Rodentibacter rarus was based on the characterization of two strains including the reference strain of taxon 17 of Bisgaard. Rodentibacter mrazii included Bisgaard taxon 21. In addition, the species Rodentibacter heidelbergensis and Rodentibacter trehalosifermentans were outlined. The eight validly published species names could be separated by phenotypic characteristics. DNA G + C content (mol%) : 40.0–41.0. Type species : Rodentibacter pneumotropicus Adhikary et al. 2017a VP (basonym: Pasteurella pneumotropica Jawetz 1950 AL ).

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Bacteria and Bacterial Diagnostics

Christensen,

Nicklas

2024

Laboratory Animal Science and Medicine

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Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice

Cited by 12 publications

References 32 publications

A Rodentibacter heylii strain lacking all known RTX toxin genes is highly virulent in C57BL/6 and BALB/c mice in contrast to Muribacter muris

A Rodentibacter heylii strain lacking all known RTX toxin genes is highly virulent in C57BL/6 and BALB/c mice in contrast to Muribacter muris

Rodentibacter

Bacteria and Bacterial Diagnostics

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