Sensitive and Label-Free Detection of DNA by Surface Plasmon Resonance

Li, Yongjun; Xiang, Juan; Zhou, Feimeng

doi:10.1007/s11468-007-9029-8

Cited by 23 publications

(12 citation statements)

References 85 publications

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“…The SPR and SPRI biosensors have been applied in molecular biology for the detection of proteins with a LOD ranging from picomolar to nanomolar [235][236][237][238][239][240], and detection of DNA molecules [130,[241][242][243] with the LOD as low as 54 fM with a flow injection device and as low as 1 fM when aided by signal amplification through gold nanoparticles [147,244]. Most of them are based on prism coupling design, such as Biocore series (Biocore X to Biocore 4000), SR series (7000 and 7500), Autolab SPR, Plasmonic, Spreeta, Nanofilm, Multiskop.…”

Section: Closing Remarks and Conclusionmentioning

confidence: 99%

“…Especially in the past few years, the performance of SPR biosensors increases quickly. The SPR and SPRI biosensors have been applied in molecular biology for the detection of proteins with a LOD ranging from picomolar to nanomolar [235][236][237][238][239][240], and detection of DNA molecules [130,[241][242][243] with the LOD as low as 54 fM with a flow injection device and as low as 1 fM when aided by signal amplification through gold nanoparticles [147,244]. SPR also provides sensitive and fast detection of cancer biomarkers [245][246][247][248] and other biomarkers [249][250][251] with the LOD at ng mL À1 level for medical diagnosis.…”

Section: Closing Remarks and Conclusionmentioning

confidence: 99%

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Surface plasmon resonance based biosensor technique: A review

Guo

2012

Journal of Biophotonics

390

223

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Optical Surface plasmon resonance (SPR) biosensors represent the most advanced and developed optical label‐free biosensor technology. Optical SPR biosensors are a powerful detection and analysis tool that has vast applications in environmental protection, biotechnology, medical diagnostics, drug screening, food safety and security. This article reviews the recent development of SPR biosensor techniques, including bulk SPR and localized SPR (LSPR) biosensors, for detecting interactions between an analyte of interest in solution and a biomolecular recognition. The concepts of bulk and localized SPs and the working principles of both sensing techniques are introduced. Major sensing advances on biorecognition elements, measurement formats, and sensing platforms are presented. Finally, the discussions on both biosensor techniques as well as comparison of both SPR sensing techniques are made. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Closing Remarks and Conclusionmentioning

confidence: 99%

Section: Closing Remarks and Conclusionmentioning

confidence: 99%

Surface plasmon resonance based biosensor technique: A review

Guo

2012

Journal of Biophotonics

390

223

View full text Add to dashboard Cite

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“…Moreover, it can detect analytes in real time avoiding the use of labels or amplification steps. It represents an user-friendly and robust analytical tool for the analysis of oligonucleotides in minimally-processed samples as previously demonstrated by detection of DNA targets (Li et al 2007;Vaisocherová et al) with a specificity up to single mismatches (Carrascosa et al 2009), and various RNA types, such as microRNAs (Lee et al 2008;Nasheri et al 2011;Sipova et al 2010;Zhou et al 2011), triplex-forming RNAs (Carrascosa et al 2012), ribosomal-RNA (Joung et al 2008;Nelson et al 2002) as well as other RNA sequences (Boucard et al 2006;Mandir et al 2009;Nair et al 2000). However, to the best of our knowledge, it has never been applied to the direct quantitative analysis of RNA spliced variants.…”

Section: Introductionmentioning

confidence: 99%

Quantitative evaluation of alternatively spliced mRNA isoforms by label-free real-time plasmonic sensing

Huertas

Carrascosa

Bonnal

et al. 2016

Biosensors and Bioelectronics

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Alternative splicing of mRNA precursors enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status. Its deregulation is strongly linked to disease onset and progression. Current methodologies for monitoring alternative splicing demand elaborate procedures and often present difficulties in discerning between closely related isoforms, e.g. due to cross-hybridization during their detection. Herein, we report a general methodology using a Surface Plasmon Resonance (SPR) biosensor for label-free monitoring of alternative splicing events in real-time, without any cDNA synthesis or PCR amplification requirements. We applied this methodology to RNA isolated from HeLa cells for the quantification of alternatively spliced isoforms of the Fas gene, involved in cancer progression through regulation of programmed cell death. We demonstrate that our methodology is isoform-specific, with virtually no cross-hybridization, achieving limits of detection (LODs) in the picoMolar (pM) range. Similar results were obtained for the detection of the BCL-X gene mRNA isoforms. The results were independently validated by RT-qPCR, with excellent concordance in the determination of isoform ratios. The simplicity and robustness of this biosensor technology can greatly facilitate the exploration of alternative splicing biomarkers in disease diagnosis and therapy.

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“…Label-free methods are becoming an important tool for biological analysis due to its inherent merits. Among the methods routinely employed are autofluorescence detection, surface plasmon resonance (SPR) (7)(8)(9), ellipsometry (10,11), interference (12,13) and acoustic wave detection (14,15), mass spectrometry (16,17), atomic force microscopy (18,19), and electrochemical methods (20)(21)(22). The most widely used label-free detection method is SPR.…”

Section: Introductionmentioning

confidence: 99%

Autofluorescence Detection in Analytical Chemistry and Biochemistry

Seeger

2010

Applied Spectroscopy Reviews

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Label-free detection of molecules and particles based on native fluorescence excited at the ultraviolet (UV) region of the electromagnetic spectrum shows great potential for molecular analysis in life sciences. It offers simple, low-cost, and fast methods for sensitive detection of important biological analytes in nature forms. This article reviews the most significant research on label-free native fluorescence detection in analytical chemistry and biochemistry, with particular focus on the instrumental requirements and applications that cover papers published to the beginning of 2009. Future development directions for label-free optical biosensors based on native fluorescence are discussed.

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Sensitive and Label-Free Detection of DNA by Surface Plasmon Resonance

Cited by 23 publications

References 85 publications

Surface plasmon resonance based biosensor technique: A review

Surface plasmon resonance based biosensor technique: A review

Quantitative evaluation of alternatively spliced mRNA isoforms by label-free real-time plasmonic sensing

Autofluorescence Detection in Analytical Chemistry and Biochemistry

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