Sensitive and label-free electrochemical lead ion biosensor based on a DNAzyme triggered G-quadruplex/hemin conformation

Wang, LeLe; Wen, Yanli; Li, Lanying; Yang, Xue; Jia, Nengqin; Li, Wen; Meng, Jiaoran; Duan, Manlei; Sun, Xiao‐Guang; Liu, Gang

doi:10.1016/j.bios.2018.04.054

Cited by 67 publications

(24 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The properties of DNA molecules have also been exploited to form dendritic structure DNA (DSDNA) [ 52 ] and hemin/G-quadruplex nanowires [ 53 ] in amplification strategies for electrochemical affinity biosensors. It is worth to remark that TSPs have been used mostly as electrode modifiers [ 54 ] and DSDNA and hemin/G-quadruplex nanowires as labels [ 52 , 53 ].…”

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

“…Wang et al [ 54 ] reported a label-free Pb 2+ biosensor involving the immobilization of a specific DNAzyme (composed of a DNAzyme strand and a substrate strand) on a TSP assembled to an AuE. The TSP allowed a better control of the density and orientation of the probe thus improving the DNAzyme reaction efficiency.…”

Section: Selected Nanostructures In Electrochemical Biosensing Formentioning

confidence: 99%

“… Label-free biosensor for voltammetric determination of Pb 2+ based on the Pb-DNAzyme triggered G-quadruplex/hemin on a TSP-modified AuE. Reprinted from [ 54 ] with permission. …”

Section: Figures Scheme and Tablesmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

View full text Add to dashboard Cite

The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

show abstract

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Section: Selected Nanostructures In Electrochemical Biosensing Formentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

View full text Add to dashboard Cite

show abstract

“…[61][62][63][64] Our group designed a type of Pb 2+ -sensitive Pdots in which the polymer/dye matrices were encapsulated by polydiacetylenes (PDAs) with 15-crown-3 as the functional groups. [61][62][63][64] Our group designed a type of Pb 2+ -sensitive Pdots in which the polymer/dye matrices were encapsulated by polydiacetylenes (PDAs) with 15-crown-3 as the functional groups.…”

Section: Ion Detectionmentioning

confidence: 99%

Semiconducting polymer dots as near‐infrared fluorescent probes for bioimaging and sensing

Tsai

Chan

2018

J Chinese Chemical Soc

View full text Add to dashboard Cite

In recent years, semiconducting polymer dots (Pdots) have emerged as a new type of ultrabright fluorescent probes, which have been proved to be very useful for biomedical imaging. Pdots possess several exceptional advantages including high fluorescence brightness, fast radiative rate, excellent photostability, and negligible cytotoxicity. Among these new types of Pdots, the near‐infrared (NIR) fluorescent Pdots appear to be the most urgent and important owing to their promising deep‐tissue imaging in the clinic. This mini‐review highlights the recent progress in the design of NIR‐emitting Pdots and their biomedical applications both in vitro and in vivo.

show abstract

“…Alternatively, the use of DNAzyme, which are some G‐rich DNA aptamers, have been regarded to be a label‐free strategy . Meanwhile, DNAzyme have been accounted to strongly bind hemin to form DNAzymes with peroxidase‐like activity . More favourably, the hemin‐binding aptamers (DNAzyme) exhibit catalytic behaviour towards the H 2 O 2 ‐mediated oxidation in the presence of o ‐phenylenediamine (OPD) .…”

Section: Introductionmentioning

confidence: 99%

Target‐stimulated DNAzyme Concatamers Released from Aptasensor for Highly Sensitive and Specific Detection of Progesterone

Shi

Liu

2019

Electroanalysis

View full text Add to dashboard Cite

Aiming at the detection of ultralow concentration target progesterone (Pro), a novel electrochemical aptasensor based on DNAzyme concatamers signal amplification strategy was proposed. The strategy consists of target DNA strands (TDNAs), and two different hairpin DNA molecules (H1 and H2). The signal is amplified by the large amount of DNAzyme. The TDNAs modified on the electrode open H1 structures in sequence and propagate a reaction of hybridization events between two alternating hairpins (H1and H2) to obtain abundant DNAzyme concatamers. Upon target Pro introduction, a specific Pro‐TDNAs reaction was executed, thereby resulting in the release of DNAzyme concatamers from the electrode. Subsequent differential pulse voltammetry(DPV) detection of aminoazobenzene (DAP) resulting by DNAzyme catalyze the oxidation of o‐phenylenediamine (OPD) with the aid of hydrogen peroxide (H2O2). Likewise, a small amount of target Pro can efficiently induce the release of a large number of the DNAzyme from the electrode in the form of DNAzyme concatamer. Under optimal conditions, the the proposed assay presents good electrochemical responses for determination of target Pro in the range of 0.5 to 15 ng/mL with the detection limit of 0.36 ng/mL. In addition, the resulting sensor can successfully distinguish Pro from coexisting interfering substance and show good stability and high repeatability. What's more, the methodology has also been demonstrated by assaying Pro‐spiked samples in serum.

show abstract

Sensitive and label-free electrochemical lead ion biosensor based on a DNAzyme triggered G-quadruplex/hemin conformation

Cited by 67 publications

References 49 publications

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Semiconducting polymer dots as near‐infrared fluorescent probes for bioimaging and sensing

Target‐stimulated DNAzyme Concatamers Released from Aptasensor for Highly Sensitive and Specific Detection of Progesterone

Contact Info

Product

Resources

About