Sensitive and Low‐background Electrochemical Immunosensor Employing Glucose Dehydrogenase and 1,10‐Phenanthroline‐5,6‐dione

Haque, Al‐Monsur Jiaul; Kwon, Jungwook; Kim, Jihyeon; Kim, Gyeongho; Lee, Nam-Sihk; Yoon, Young Ho; Yang, Haesik

doi:10.1002/elan.202100079

Cited by 4 publications

(3 citation statements)

References 56 publications

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“…Compared with other GDHs, the catalysis of FAD-GDH does not require external cofactors. Haque et al developed an electrochemical immunosensor for the detection of PTH with FAD-GDH-based EN redox cycling ( Figure 11 A) [ 150 ]. In this study, 1,10-phenanthroline-5,6-dione (PD), a heterocyclic electroactive quinone, was used as the electron mediator and ITO with a low electrocatalytic activity was employed as the working electrode to decrease the background current from the reduction of O 2 .…”

Section: Oxidoreductases As the Signal Labels Of Electrochemical Immu...mentioning

confidence: 99%

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Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Xia,

Gao,

Zhang

et al. 2024

Molecules

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Enzyme-linked electrochemical immunosensors have attracted considerable attention for the sensitive and selective detection of various targets in clinical diagnosis, food quality control, and environmental analysis. In order to improve the performances of conventional immunoassays, significant efforts have been made to couple enzyme-linked or nanozyme-based catalysis and redox cycling for signal amplification. The current review summarizes the recent advances in the development of enzyme- or nanozyme-based electrochemical immunosensors with redox cycling for signal amplification. The special features of redox cycling reactions and their synergistic functions in signal amplification are discussed. Additionally, the current challenges and future directions of enzyme- or nanozyme-based electrochemical immunosensors with redox cycling are addressed.

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Section: Oxidoreductases As the Signal Labels Of Electrochemical Immu...mentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Xia,

Gao,

Zhang

et al. 2024

Molecules

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“…To date, most AECs use intact antibodies, particularly immunoglobulin G (IgG) antibodies [26][27][28]. IgG antibodies have a four-chain structure in which two heavy chains (Hc) and two light chains (Lc) are combined with non-covalent and disulfide bonds.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing

Oda

Asano

2022

IJMS

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Antibody–enzyme complexes (AECs) are ideal molecular recognition elements for immunosensing applications. One molecule possesses both a binding ability to specific targets and catalytic activity to gain signals, particularly oxidoreductases, which can be integrated into rapid and sensitive electrochemical measurements. The development of AECs using fragment antibodies rather than intact antibodies, such as immunoglobulin G (IgG), has attracted attention for overcoming the ethical and cost issues associated with the production of intact antibodies. Conventionally, chemical conjugation has been used to fabricate AECs; however, controlling stoichiometric conjugation using this method is difficult. To prepare homogeneous AECs, methods based on direct fusion and enzymatic conjugation have been developed, and more convenient methods using Catcher/Tag systems as coupling modules have been reported. In this review, we summarize the methods for fabricating AECs using fragment antibodies developed for sensing applications and discuss the advantages and disadvantages of each method.

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Fabrication of Fe3O4@SiO2@PDA-Ni2+ nanoparticles for one-step affinity immobilization and purification of His-tagged glucose dehydrogenase

Huang

Shen

et al. 2023

Process Biochemistry

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Sensitive and Low‐background Electrochemical Immunosensor Employing Glucose Dehydrogenase and 1,10‐Phenanthroline‐5,6‐dione

Cited by 4 publications

References 56 publications

Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing

Fabrication of Fe3O4@SiO2@PDA-Ni2+ nanoparticles for one-step affinity immobilization and purification of His-tagged glucose dehydrogenase

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