Sensitive and rapid detection of bacterial endotoxin with a functional carbon nanotube field-effect transistor biosensor

Cui, Qing; Li, Jiahao; Li, Yutao; Tang, Lina; Li, Kun; Li, Tingxian; Chen, Xiaohua; Zhang, Zhiyong; Zhang, Guo-Jun

doi:10.1016/j.talanta.2023.125035

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…It also allowed for rapid analysis of Gram-negative bacterial infections in blood samples, demonstrating superior diagnostic accuracy (AUC = 0.990) based on the receiver operating characteristic curve (ROC). 51…”

Section: Nanodiagnostic Techniques For Early Detection Of Bacterial S...mentioning

confidence: 99%

Recent advances and prospects in nanomaterials for bacterial sepsis management

Zhou,

Liu,

et al. 2023

J. Mater. Chem. B

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Section: Nanodiagnostic Techniques For Early Detection Of Bacterial S...mentioning

confidence: 99%

Recent advances and prospects in nanomaterials for bacterial sepsis management

Zhou,

Liu,

et al. 2023

J. Mater. Chem. B

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“…6,7 Nucleic acid-based molecular biology methods such as gene sequencing demand bulky instruments and trained operators, restricting their application to centralized laboratories. 8,9 Despite the ongoing evolution of molecular hybridization, 10,11 DNA chips, 12,13 and nucleic acid biosensors, 14,15 technologies are advancing toward expedited response, reduced costs, and simplified miniaturization, the absence of a universal target compels patients with bacterial infections to undergo a complex pathogen identification procedure, thus delaying treatment.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The culture method continues to be the gold standard for the clinical detection of bacteria in hospitals. However, this method has significant limitations including a slow process time, low detection rates, which are a result of generational time delay, and the inability to cultivate certain bacteria. , Time-of-flight mass spectrometry provides faster bacterial identification but necessitates pure cultured bacteria and expensive equipment, hindering point-of-care testing (POCT). , Furthermore, immunological methods pose challenges due to low detection sensitivity and difficulties in procuring monoclonal antibodies. , Nucleic acid-based molecular biology methods such as gene sequencing demand bulky instruments and trained operators, restricting their application to centralized laboratories. , Despite the ongoing evolution of molecular hybridization, , DNA chips, , and nucleic acid biosensors, , technologies are advancing toward expedited response, reduced costs, and simplified miniaturization, the absence of a universal target compels patients with bacterial infections to undergo a complex pathogen identification procedure, thus delaying treatment.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Broad-Spectrum Pathogenic Bacteria Based on Highly Sensitive Proton Response of the Nucleic Acid Amplification SPQC Platform

Liao,

Liu,

Feng

et al. 2024

Anal. Chem.

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Pathogenic bacteria significantly contribute to elevated morbidity and mortality rates, highlighting the urgent need for early and precise detection. Currently, there is a paucity of effective broad-spectrum methods for detecting pathogenic bacteria. We have developed an innovative proton-responsive series piezoelectric quartz crystal (PR-SPQC) platform for the broad-spectrum identification of pathogenic bacteria. This was achieved by retrieving and aligning sequences from the NCBI GenBank database to identify and validate 16S rRNA oligonucleotide sequences that are signatures of pathogenic bacteria but absent in humans or fungi. The hyperbranched rolling circle amplification, activated exclusively by the screened target, exponentially generates protons that are detected by SPQC through a 2D polyaniline (PANI) film. The PR-SPQC platform demonstrates broad-spectrum capabilities in detecting pathogenic bacteria, with a detection limit of 2 CFU/mL within 90 min. Clinical testing of blood samples yielded satisfactory results. With its advantages in miniaturization, cost efficiency, and suitability for point-of-care testing, PR-SPQC has the potential to be extensively used for the rapid identification of diverse pathogenic bacteria within clinical practice and public health sectors.

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The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

Almeida Gonzalez,

Hernandez Ojeda,

Corcho‐Valdés

et al. 2024

Analysis & Sensing

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Carbon nano‐onions (CNOs) promise to improve the range of applications of carbon materials for electroanalytical applications. In this review we explore the synthesis, characterization, and electrochemical applications of CNOs. CNOs‐based sensors present impressive features, including low detection limits in the femtogram per milliliter range, a wide linear detection range spanning up to 7 orders of magnitude, exceptional selectivity, reproducibility, and stability. Synthetic methods and characterization techniques for CNOs were thoroughly examined, shedding light on their pivotal role in biosensing technologies. Comparative analyses with other carbon materials underscore CNOs' competitive performance, either surpassing or matching many counterparts. Despite their relatively recent integration in biosensing applications, CNOs exhibit comparable or superior results concerning other carbon‐based materials. Indeed, the incorporation of CNOs into hybrid nanocomposites have shown promising outcomes, indicating a synergistic potential for future advancements in biosensing technologies. Our review provides a broad approach of the application of CNOs to the field, with emphasis in breakthroughs of the last 5 years.

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Sensitive and rapid detection of bacterial endotoxin with a functional carbon nanotube field-effect transistor biosensor

Cited by 7 publications

References 42 publications

Recent advances and prospects in nanomaterials for bacterial sepsis management

Recent advances and prospects in nanomaterials for bacterial sepsis management

Rapid Detection of Broad-Spectrum Pathogenic Bacteria Based on Highly Sensitive Proton Response of the Nucleic Acid Amplification SPQC Platform

The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

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