Sensitive and selective determination of butyl benzyl phthalate from environmental samples using an enzyme immunoassay

Li, Ming; Cui, Yin; Liu, Zhenjiang; Xue, Yan; Zhao, Ru-Jin; Li, Yong; Du, Daolin

doi:10.1016/j.scitotenv.2019.06.037

Cited by 19 publications

(11 citation statements)

References 38 publications

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“…In the overall development and application of immunoassays for OTA, immunochromatography has received extensive attention due to its advantages in simplicity, readability, and portability 19–22 . Shu et al .…”

Section: Introductionmentioning

confidence: 99%

“…18 In the overall development and application of immunoassays for OTA, immunochromatography has received extensive attention due to its advantages in simplicity, readability, and portability. [19][20][21][22] Shu et al used IC to measure OTA levels in food and animal feed with a sensitivity of 5 ng mL −1 and a detection time of only 10 min. 23 Li et al produced a multi-component chromatographic test strip for the simultaneous detection of AFB 1 , OTA, and ZEN, in which the LOD for OTA was 0.5 ng mL −1 .…”

Section: Introductionmentioning

confidence: 99%

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Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

et al. 2020

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BACKGROUND The toxicity and health risks of mycotoxins have encouraged increased awareness and strict monitoring of these contaminants in agricultural by‐products. In this paper, we developed and tested a sensitive, selective, and self‐assembling immunochromatographic (IC) strip for on‐site detection of ochratoxin A (OTA). We were able to demonstrate semi‐quantitative and quantitative detection of OTA in agricultural by‐product samples. RESULTS The optimized IC strip had a limit of detection (LOD) of 0.5 ng mL−1 OTA using the naked eye for semi‐quantitative detection. When a digitized strip reader was used to achieve quantitative results, the LOD for OTA was reduced to 0.1 ng mL−1 with a linear detection range of 0.1–10 ng mL−1. We also evaluated the specificity, stability, accuracy, and precision of the IC strip and demonstrated high performance in all of these areas. We then confirmed the ability of the IC strip to visually detect OTA contamination in authentic agricultural by‐product samples from the markets in China. Furthermore, quantitative detection of OTA using the IC strip showed that the concentration of OTA ranged from 1.7 to 101.3 ng g−1 in the positive agricultural by‐product samples, correlating well with the measurements obtained via liquid chromatography with tandem mass spectrometry. CONCLUSION The results indicated that this proposed IC strip was capable of sensitive, semi‐quantitative, quantitative, and on‐site detection of OTA contamination in agricultural by‐product samples. © 2020 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

et al. 2020

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“…At present, instrument‐based methods, such as high‐performance liquid chromatography (HPLC) 6 and HPLC‐mass spectrometry 7,8 have been used as reference and accurate methods for detecting ZEN. However, these methods are expensive, requring professional operation and complex pretreatment 9–12 . In addition to the instrument‐based methods, immunoassays have attracted widespread concern for ZEN monitoring, such as enzyme‐linked immunosorbent assays (ELISA), fluorescence immunoassays and immunochromatography assays 13–16 .…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive monitoring strategy of PCR‐like levels for zearalenone contamination based DNA barcode

Xia

Qiu

et al. 2021

J Sci Food Agric

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BACKGROUND The ultrasensitive monitoring strategy of zearalenone (ZEN) is essential and desirable for food safety and human health. In the present study, a coupling of gold nanoparticles‐DNA barcode and direct competitive immunoassay‐based real‐time polymerase chain reaction signal amplification (RT‐IPCR) for ZEN close to the sensitivity of PCR‐like levels is described and evaluated. RESULTS The RT‐IPCR benefited from the use of a DNA barcode and RT‐PCR detection strategy, thus resulting in ultrasensitive and simple detection for ZEN. Under the optimal RT‐IPCR, the linear range of detection was from 0.5 to 1000 pg mL−1 and the limit of detection was 0.5 pg mL−1, which was 400‐fold lower than the enzyme‐linked immunosorbent assay. The detection procedure was simplified and the detection time was shortened. The specificity, accuracy and precision of the RT‐IPCR confirmed a high performance. ZEN‐positive contamination levels were from 0.056 to 152.12 ng g−1 by the RT‐IPCR, which was demonstrated to be highly reliable by liquid chromatography‐tandem mass spectrometry. CONCLUSION The proposed RT‐IPCR could be used as an alternative for detecting ZEN with satisfactory ultrasensitivity, simplicity, low cost and high‐throughput. The present study could provide a strategy for the ultrasensitive detection of the small molecule with a simple and practical approach, which has significant appeal and application prospects.

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“…Of the variety of immunoassays, the immunochromatographic assay (ICA) has attracted more attention. It is widely used for detecting contamination, because of its outstanding characteristics of simplicity, readability, and portability [22,23].…”

Section: Introductionmentioning

confidence: 99%

Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay

Xia

Mao

Yang

et al. 2020

Toxins

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Zearalenone (ZEN) is a prevalent mycotoxin that needs intensive monitoring. A semi-quantitative and quantitative immunochromatographic assay (ICA) was assembled for investigating ZEN contamination in 187 samples of cereal and their products from China in 2019. The semi-quantitative detection model had a limit of detection (LOD) of 0.50 ng/mL with visual judgment and could be completely inhibited within 5 min at 3.0 ng/mL ZEN. The quantitative detection model had a lower LOD of 0.25 ng/mL, and ZEN could be accurately and digitally detected from 0.25–4.0 ng/mL. The ICA method had a high sensitivity, specificity, and accuracy for on-site ZEN detection. For investigation of the authentic samples, the ZEN-positive rate was 62.6%, and the ZEN-positive levels ranged from 2.7 to 867.0 ng/g, with an average ZEN-positive level being 85.0 ng/g. Of the ZEN-positive samples, 6.0% exceeded the values of the limit levels. The ZEN-positive samples were confirmed to be highly correlated using LC-MS/MS (R2 = 0.9794). This study could provide an efficiency and accuracy approach for ZEN in order to achieve visual and digitized on-site investigation. This significant information about the ZEN contamination levels might contribute to monitoring mycotoxin occurrence and for ensuring food safety.

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Sensitive and selective determination of butyl benzyl phthalate from environmental samples using an enzyme immunoassay

Cited by 19 publications

References 38 publications

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

Ultrasensitive monitoring strategy of PCR‐like levels for zearalenone contamination based DNA barcode

Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay

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