Sensitive and selective DNA detection based on the combination of hairpin-type probe with endonuclease/GNP signal amplification using quartz-crystal-microbalance transduction

Fei, Yuehua; Jin, Xiaoyong; Wu, Zai‐Sheng; Zhang, Songbai; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.aca.2011.02.040

Cited by 17 publications

(3 citation statements)

References 66 publications

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“…Since REases cleave both strands of the double-stranded DNA substrate, the use of REases for EATR with conventional probes will result in the cleavage of the target along with the probe, thus target recycling would not be possible. [89][90][91][92][93] To overcome this limitation, 3J probes are used. A group of Sintim suggested using 3J probes that operate via template enhanced hybridization processes (TeHyP).…”

Section: Restriction Endonuclease-assisted Assaysmentioning

confidence: 99%

Enzyme-assisted target recycling (EATR) for nucleic acid detection

2014

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Fast, reliable and sensitive methods for nucleic acid detection are of growing practical interest with respect to molecular diagnostics of cancer, infectious and genetic diseases. Currently, PCR-based and other target amplification strategies are most extensively used in practice. At the same time, such assays have limitations that can be overcome by alternative approaches. There is a recent explosion in the design of methods that amplify the signal produced by a nucleic acid target, without changing its copy number. This review aims at systematization and critical analysis of the enzyme-assisted target recycling (EATR) signal amplification technique. The approach uses nucleases to recognize and cleave the probe-target complex. Cleavage reactions produce a detectable signal. The advantages of such techniques are potentially low sensitivity to contamination and lack of the requirement of a thermal cycler. Nucleases used for EATR include sequence-dependent restriction or nicking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII, AP endonuclease, duplex-specific nuclease, DNase I, or T7 exonuclease. EATR-based assays are potentially useful for point-of-care diagnostics, single nucleotide polymorphisms genotyping and microRNA analysis. Specificity, limit of detection and the potential impact of EATR strategies on molecular diagnostics are discussed.

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Section: Restriction Endonuclease-assisted Assaysmentioning

confidence: 99%

Enzyme-assisted target recycling (EATR) for nucleic acid detection

2014

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“…5 However, it suffers from involving complex reactions and is hard to apply in vivo or even in situ. Nowadays, many techniques including uorescence, 6 electrochemiluminescence, 7 electrochemistry, 8 and quartz crystal microbalance 9 have been developed for DNA detection. In these methods, labels are usually necessary.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive and in situ DNA detection in various pH environments based on a microfiber with a graphene oxide linking layer

Huang

Guo

2017

RSC Adv.

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“…29 In addition, pathogens can be detected by quantitatively assaying their unique nucleic acid sequences. [30][31][32][33][34][35] Various techniques such as electrochemiluminescence, 36 electrochemistry, 37,38 fuorescence 39,40 and quartz crystal microbalance 41 have been developed for DNA detection. Among them, electrochemical genosensor possesses enormous promise due to its fast, simple and inexpensive characters in specic DNA target sequences analysis.…”

Section: Introductionmentioning

confidence: 99%

Label-free electrochemical impedance genosensor based on 1-aminopyrene/graphene hybrids

et al. 2013

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In this work, we proposed a novel simple protocol for preparing 1-aminopyrene/graphene (ApG) hybrids for fabricating label-free electrochemical impedance genosensor. Graphene, with the structure of a single-atom-thick sheet of sp(2)-bonded carbon atoms, was anchored to 1-aminopyrene (1-Ap) with the pyrenyl group viaπ-stacking interaction. The morphology, conductivity, and interaction of ApG hybrids were characterized by transmission electron microscopy (TEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), UV-visible (UV-vis) and fluorescence spectra. The amino-substituted oligonucleotide probe was conjugated to 1-Ap by the cross-linker glutaraldehyde. The DNA hybridization reaction of oligonucleotide probe with target DNA was monitored by EIS. Under optimum conditions, the proposed biosensor exhibited high sensitivity and a low detection limit for detecting the complementary oligonucleotide. The target oligonucleotide could be quantified in a wide range of 1.0 × 10(-12) to 1.0 × 10(-8) M with good linearity (R = 0.9900) and low detection limit of 4.5 × 10(-13) M (S/N = 3).

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Sensitive and selective DNA detection based on the combination of hairpin-type probe with endonuclease/GNP signal amplification using quartz-crystal-microbalance transduction

Cited by 17 publications

References 66 publications

Enzyme-assisted target recycling (EATR) for nucleic acid detection

Enzyme-assisted target recycling (EATR) for nucleic acid detection

Ultrasensitive and in situ DNA detection in various pH environments based on a microfiber with a graphene oxide linking layer

Label-free electrochemical impedance genosensor based on 1-aminopyrene/graphene hybrids

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