Sensitive and selective gas chromatographic methods for the quantitation of camphor, menthol and methyl salicylate from human plasma

Valdez, Jennifer S; Martin, Debra K.; Mayersohn, Michael

doi:10.1016/s0378-4347(99)00161-9

Cited by 24 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…36 Methods for quantitation of camphor in biological samples include reverse phase high performance liquid chromatography (RP/HPLC) with UV detection and gas chromatography with fl ame ionization detection (GC/FID). 38 The between -day coeffi cient of variation for camphor in this study was about 13.5%. 38 The between -day coeffi cient of variation for camphor in this study was about 13.5%.…”

Section: Methodsmentioning

confidence: 53%

Camphor (Cinnamomum camphoraT. Nees & Eberm.)

Barceloux

2008

Medical Toxicology of Natural Substances

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 53%

Camphor (Cinnamomum camphoraT. Nees & Eberm.)

Barceloux

2008

Medical Toxicology of Natural Substances

View full text Add to dashboard Cite

“…Although high pressure liquid chromatography/mass spectrometry (HPLC/MS) has been demonstrated,[8] most method development has involved gas chromatography (GC) separation, because menthol is still within the realm of a volatile organic compound, with a boiling point of 212 °C and thermal stability. [9,10] As a result, GC methods have yielded better sensitivity than LC methods by at least two orders of magnitude, especially when combined with mass spectrometric detection. In 2004, Spichiger et al incorporated solid phase microextraction (SPME) as a means to preconcentrate menthol collected in the headspace (HS) over urine and serum specimens before and after hydrolysis of menthol glucuronide adducts.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of menthol in human urine using solid phase microextraction and stable isotope dilution gas chromatography–mass spectrometry

Huang

Blount

Watson

et al. 2017

Journal of Chromatography B

View full text Add to dashboard Cite

To accurately measure menthol levels in human urine, we developed a method using gas chromatography/electron ionization mass spectrometry with menthol-d4 stable isotope internal standardization. We used solid phase microextraction (SPME) headspace sampling for collection, preconcentration and automation. Conjugated forms of menthol were released using β-glucuronidase/sulfatase to allow for measuring total menthol. Additionally, we processed the specimens without using β-glucuronidase/sulfatase to quantify the levels of unconjugated (free) menthol in urine. This method was developed to verify mentholated cigarette smoking status to study the influence of menthol on smoking behaviour and exposure. This objective was accomplished with this method, which has no carryover or memory from the SPME fiber assembly, a method detection limit of 0.0017 μg/mL, a broad linear range of 0.002–0.5 μg/mL for free menthol and 0.01 – 10 μg/mL for total menthol, a 7.6% precision and 88.5% accuracy, and an analysis runtime of 17 min. We applied this method in analysis of urine specimens collected from cigarette smokers who smoke either mentholated or non-mentholated cigarettes. Among these smokers, the average total urinary menthol levels was three-fold higher (p <0.001) among mentholated cigarette smokers compared with non-mentholated cigarette smokers.

show abstract

“…In humans, few studies were performed to elucidate the pharmacokinetic profile of menthol and its main metabolite M-G, but not of other phase-I metabolites. For the quantification of menthol and M-G after enzymatic hydrolysis in plasma and urine different methods including liquid-liquid extraction or headspace sampling followed by analysis with GC and GC-MS were proposed [4][5][6][7][8][9][10]. In plasma unconjugated menthol was only detected after a transdermal application [5].…”

Section: Introductionmentioning

confidence: 99%

“…For the quantification of menthol and M-G after enzymatic hydrolysis in plasma and urine different methods including liquid-liquid extraction or headspace sampling followed by analysis with GC and GC-MS were proposed [4][5][6][7][8][9][10]. In plasma unconjugated menthol was only detected after a transdermal application [5]. The problem of methods basing on liquid-liquid extraction consists in loosing the lipophilic analytes by volatilisation [9], resulting in loss of recovery, sensitivity and precision.…”

Section: Introductionmentioning

confidence: 99%