Sensitive and semi-quantitative TaqMan™ real-time polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed

Brodmann, Peter; Moor, Dominik

doi:10.1016/s0309-1740(02)00253-x

Cited by 119 publications

(72 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Amplification and the size of amplicon has, in some studies, rates are affected by many factors, amongst which appeared to override all other considerations, include the number of target molecules, the compromising assay specificity in some instances sequence of the target, the buffer and thermostable (Brodmann and Moor 2003;Dooley et al 2004; polymerase, the cycling conditions, the primer Mendoza- Romero et al 2004). Brodmann and Moor designed assays for the smallest possible amplicons to ensure amplification of highly processed feeds.…”

Section: Introductionmentioning

confidence: 99%

Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction

Hird¹,

Chisholm²,

Sánchez

et al. 2006

Food Additives and Contaminants

102

View full text Add to dashboard Cite

The design of real-time polymerase chain reaction (PCR) assays for the detection of meat in processed products has focused on using small amplicons, often to the detriment of specificity. However, the relationship between amplification rates and the amplicon size for processed meat products has yet to be determined. To investigate this relationship, real-time PCR assays were designed to give a series of amplicons of increasing size. These assays were then used to assess amplification rates, in relation to amplicon size, in processed meat matrices. Although the most sensitive assays were those that used the smallest amplicons, amplification was still observed using amplicons of 351 base pairs for highly processed samples. It was found, therefore, that although in general, amplicons should be as small as possible, larger amplicons give efficient amplification and that small amplicons should not be chosen if they compromise assay specificity.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction

Hird¹,

Chisholm²,

Sánchez

et al. 2006

Food Additives and Contaminants

102

View full text Add to dashboard Cite

show abstract

“…Prado et al carried out an inter-laboratory study using the TaqMan-qPCR system, which detected the amount of 0.1% w/w bovine MBM or mixtures with different raw materials of animal origin in ruminant feedstuffs, which corresponds to the same detection limit found herein [40]. Brodmann and Moor, compared the sensitivity of TaqMan-qPCR to microscopic analysis in detecting MBM, and concluded that qPCR tests cannot compete with the microscopic method [38]. A detection limit around 1% in meat and bone-meal samples may be insufficient to eradicate BSE.…”

Section: Spiked Feeds Concentration Of Mbm (%) W/w Addedmentioning

confidence: 87%

“…The precision of the measurement decreased with the number of copies of the target sequence in the genome and harsher conditions during the processing of meat-and-bone meal, which lead to highly fragmented DNA. An alternative is to evaluate the mass concentrated from serial dilutions of each DNA template to establish the method sensitivity, as was conducted in this study [38].…”

Section: Spiked Feeds Concentration Of Mbm (%) W/w Addedmentioning

confidence: 99%

Detection of Animal Products in Ruminant Feeds by Microscopy and Real Time PCR

Golinelli¹,

Silva²

2015

J Veterinar Sci Technol

View full text Add to dashboard Cite

This study evaluated the performance of the TaqManTM real-time PCR assay to detection of DNA from not allowed-animal derivatives in reference feeds samples. The results of qPCR were compared with the microscopy, only method validated to control the presence of animal proteins, according the European Communities. The qPCR tests targeting 12S rRNA from cows, sheep, porcine and chickens and cytochrome b region from caprine in feeds were able to detect half the amount (0.0125% w/w) of meat-and-bone meal (MBM) that could be detected by microscopy in samples spiked with MBM. Although cross-contamination in feeds and food processing plants is an unexceptional problem, the presence of traces of prohibited animal products in feedstuffs is an alert to potential impact on herd and human health, because it has been associated with the transmission of transmissible spongiform encephalopathy (TSE). These results indicate that a combination of qPCR tests and microscopic analysis could be used to ensure the safety of feedstuffs, allowing the identification of the animal species of the derivative and even the kind of tissue added to the feed, providing useful information for sanitation inspection authorities in this country.

show abstract

“…PCR primers designed on the basis of sequences of short interspersed repetitive elements have been successfully used to detect low contamination levels of feed by animal material (Tajima et al, 2002). Recently, with the emergence of real-time PCR technology, PCR methods for mitochondrial encoded targets (Lahiff et al, 2002) or those in nuclear sequences (Brodmann and Moor, 2003) based on the use of Taqman probes, have been reported for the detection of bovine material in MBM and feedstuffs containing animal MBM (Castelló et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program

Corona¹,

Lleonard²,

Carpio³

et al. 2013

Span. j. agric. res.

View full text Add to dashboard Cite

The rapid identification of residues of mammalian materials in animal feedstuffs is important for the effective control of feed as a potential source of transmission of bovine spongiform encephalopathy (BSE). It is included in the monitoring program developed to avoid the appearance of this disease. In the present work a PCR analysis was tested for the detection of mammalian residues (bovine, caprine, ovine and porcine). The DNA extraction method utilised guanidium thiocianate, and primers flanking a conserved region of mitochondrial DNA were synthesized for each species. PCR tests were specific for each species and allowed detection in feedstuffs of levels as low as 1% of bovine DNA and 1.5% of ovine, caprine and porcine DNA. These PCR tests may allow the rapid detection of residues of these species constituting a powerful tool against BSE

show abstract

Sensitive and semi-quantitative TaqMan™ real-time polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed

Cited by 119 publications

References 21 publications

Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction

Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction

Detection of Animal Products in Ruminant Feeds by Microscopy and Real Time PCR

Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program

Contact Info

Product

Resources

About