Sensitive and specific multiresidue methods for the determination of pesticides of various classes in clinical and forensic toxicology

Lacassie, Éric; Marquet, Pierre; Gaulier, Jean‐Michel; Dreyfuss, Marie-Françoise; Lachâtre, Gérard

doi:10.1016/s0379-0738(01)00461-3

Cited by 81 publications

(38 citation statements)

References 22 publications

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“…Outros tipos de cromatografia sugeridos para a detecção do aldicarb e seus metabólitos são a cromatografia líquida de alta resolução para amostras biológicas (Lacassie et al, 2001;Melito, 2003), água (Miles e Delfino, 1998) e vegetais (Minelli et al, 1997;Nunes et al, 1998), e a cromatografia a gás para amostras de água ou biológicas (Harper et al, 1998). No entanto, essas análises cromatográficas requerem equipamentos sofisticados, investimento em infra-estrutura e material e pessoal técnico especializado (Moraes, 2002), fatos que aumentam os custos da análise química de cada amostra e, muitas vezes, inviabilizam sua execução na rotina dos laboratórios de análises toxicológicas de países em desenvolvimento.…”

Section: Discussionunclassified

Cromatografia em camada delgada para o diagnóstico da intoxicação por aldicarb ("chumbinho") em cães e gatos

Xavier

Righi

Flório

2007

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliou-se a cromatografia em camada delgada (CCD) como método de diagnóstico toxicológico para os casos de intoxicação por aldicarb em cães e gatos, utilizando-se 50 amostras de conteúdo gástrico obtidas durante a necropsia e 50 amostras de alimentos utilizados como iscas para intoxicar criminalmente os animais. Todas as amostras resultaram positivas para o aldicarb, mostrando ser a CCD uma técnica qualitativa eficiente, rápida e de baixo custo, com uso potencial na toxicologia veterinária forense.Palavras-chave: cão, gato, cromatografia em camada delgada, aldicarb, conteúdo gástrico, intoxicação ABSTRACT The present study concerns about the identification of aldicarb residues using thin-layer chromatography (TLC) in 50 samples of gastric content obtained from the necropsy of dogs and cats and

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Section: Discussionunclassified

Cromatografia em camada delgada para o diagnóstico da intoxicação por aldicarb ("chumbinho") em cães e gatos

Xavier

Righi

Flório

2007

Arq. Bras. Med. Vet. Zootec.

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“…In connection to GC analysis, a single original and sensitive multiresidue method has been developed for the detection and quantification of 61 pesticides of toxicological significance where GC-MS was found to be the best for Ura detection in volatile pesticides in human biological matrixes as reported earlier [15].…”

Section: Hybrid Separation Techniques Used For Sensitive Analysis Of mentioning

confidence: 93%

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

2015

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The most important objectives that are frequently found in bio-analytical chemistry involve applying tools to relevant medical/biological problems and refining these applications. Developing a reliable sample preparation step, for the medical and biological fields is another primary objective in analytical chemistry, in order to extract and isolate the analytes of interest from complex biological matrices. Since, main inborn errors of metabolism (IEM) diagnosable through uracil analysis and the therapeutic monitoring of toxic 5-fluoruracil (an important anticancerous drug) in dihydropyrimidine dehydrogenase deficient patients, require an ultra-sensitive, reproducible, selective, and accurate analytical techniques for their measurements. Therefore, keeping in view, the diagnostic value of uracil and 5-fluoruracil measurements, this article refines several analytical techniques involved in selective recognition and quantification of uracil and 5-fluoruracil from biological and pharmaceutical samples. The prospective study revealed that implementation of molecularly imprinted polymer as a solid-phase material for sample preparation and preconcentration of uracil and 5-fluoruracil had proven to be effective as it could obviates problems related to tedious separation techniques, owing to protein binding and drastic interferences, from the complex matrices in real samples such as blood plasma, serum samples.

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“…9,15 These compounds are commonly found in foods and beverages in cases of accidental or intentional intoxication. 17 Over the last years, 157 cases involving aldicarb-contaminated commercial products, and liquid or solid foodstuffs (meat, coffee, milk, soup, juice, cookies, and chocolate) were reported in the Rio de Janeiro State. 18 The commercial formulations of these pesticides present a grayish color and therefore dark colored beverages such as juices and chocolate drinks are frequently employed in food poisoning.…”

Section: Introductionmentioning

confidence: 99%

“…17,[30][31][32] The identification of these compounds in different matrices as well as their quantification are of interest in the criminal field since the number of human 3,17,[33][34][35][36] 53 or supercritical fluid extraction 54 followed by gas 20,50,[55][56][57][58][59][60] or liquid chromatography. 1,19,48,49,52,53,[61][62][63][64][65][66][67][68][69][70] The low volatility and the thermolability of these compounds and the high polarities of the transformation products obviously favor the use of column liquid chromatography (LC) as the separation technique.…”

Section: Introductionmentioning

confidence: 99%

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Determination of carbamates in beverages by liquid-liquid extraction with low temperature partitioning and liquid chromatography

Goulart¹,

Alves²,

Paula³

et al. 2012

J. Braz. Chem. Soc.

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Carbamatos são os pesticidas mais frequentemente encontrados em alimentos e bebidas de coloração escura em casos de intoxicação acidental ou intencional. Neste trabalho, extração líquido-líquido com partição em baixa temperatura (LLE-LTP) foi otimizada e validada para determinação dos carbamatos aldicarb, carbofuran e carbaril em bebidas de sucos de uva e leite achocolatado. Este método envolve a extração com acetonitrila, partição líquido-líquido em baixa temperatura e análise por cromatografia líquida de alta eficiência com detecção ultravioleta (HPLC-UV). O método é rápido, eficiente e de baixo custo, emprega pequenos volumes de solvente por amostra e não necessita de limpeza dos extratos. O método de extração foi seletivo e apresentou porcentagens de extração acima de 90%. As premissas relacionadas com os testes estatísticos de linearidade foram verificadas e confirmadas. O método de extração e análise foi validado com resultados satisfatórios e pode ser aplicado em análises de rotina e forenses.Carbamates are the pesticides most commonly found in dark colored foods and beverages in cases of accidental or intentional poisoning. In this work, the liquid-liquid extraction with low temperature partitioning (LLE-LTP) was optimized and validated for determination of the carbamates aldicarb, carbofuran and carbaryl in grape juice and chocolate milk beverages. This method involved extraction with acetonitrile, liquid-liquid partition at low temperature and the analysis by high performance liquid chromatography with ultraviolet detection (HPLC-UV). The method is rapid, efficient and of low-cost, employing small volumes of solvent per sample and requiring no cleanup of the extracts. The extraction methodology was selective and presented recovery percentages above 90%. The premises related to the statistical linearity tests were checked and confirmed. The method of extraction and analysis was validated with satisfactory results, and may be applied in forensic and routine analysis Keywords: carbamates, low temperature partitioning, high performance liquid chromatography, sample preparation, validation IntroductionCarbamate insecticides are widely used in gardening and agriculture due to their broad spectrum of activity and relatively short environmental persistence. However, they are potentially toxic to humans, particularly in the context of intentional self-poisoning. 1,2 Carbamates are found as commercialized products for agricultural practices and some compounds of this group, with their respective commercial names are: carbaryl (Sevin ® ), aldicarb (Temik ® ) and carbofuran (Furadan ® ). From these, aldicarb stands out as the main target on the list of controlled pesticides of the United States Environmental Protection Agency (EPA) due to its elevated toxicity to mammals. 3,4 The maximum residue level (MRL) of aldicarb 5,6 and carbofuran 6 in water is 10 and 7 μg L -1 , respectively. In Goulart et al. 1155 Vol. 23, No. 6, 2012 milk, the MRLs established for aldicarb, 7,8 Exposures may occur accidentally, with ...

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Sensitive and specific multiresidue methods for the determination of pesticides of various classes in clinical and forensic toxicology

Cited by 81 publications

References 22 publications

Cromatografia em camada delgada para o diagnóstico da intoxicação por aldicarb ("chumbinho") em cães e gatos

Cromatografia em camada delgada para o diagnóstico da intoxicação por aldicarb ("chumbinho") em cães e gatos

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

Determination of carbamates in beverages by liquid-liquid extraction with low temperature partitioning and liquid chromatography

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