Sensitive detection of aflatoxin B1 in foods by aptasensing-based qPCR

Sun, Jian; Ning, Xueping; Cui, Lanyu; Qin, Wenhui; Wang, Wenxu; He, Shengbin

doi:10.1016/j.foodchem.2023.137240

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Nucleic Acid Signal Amplification Techniques2

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2024

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Cited by 5 publications

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“…69,70 The nucleic acid signal amplification technique (NASAT) takes advantage of various enzymes, nucleases, and deoxyribozyme (DNAzyme) to amplify the signal, 71,72 and the integration of NASAT with aptamers can greatly improve the sensitivity of the mycotoxin assay. 66–80 NASAT is divided into enzyme-assisted nucleic acid amplification and enzyme-free amplification. Enzyme-assisted nucleic acid amplification includes polymerase chain reaction (PCR), rolling circle amplification (RCA), strand displacement amplification (SDA), nuclease-assisted amplification, and CRISPR/Cas system-related amplification.…”

Section: Nucleic Acid Signal Amplification Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in nucleic acid signal amplification-based aptasensors for sensing mycotoxins

Zhang,

Luo,

Cai

et al. 2024

Chem. Commun.

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Section: Nucleic Acid Signal Amplification Techniquesmentioning

confidence: 99%

“…2C). 77 When AFB1 is present, the template can be switched from a dumbbell-shaped structure to a linear structure. Due to the restriction, enzymes cannot act on the linear template, and the linear DNA templates are subjected to qPCR for the sensitive quantification of AFB1.…”

Section: Nucleic Acid Signal Amplification Techniquesmentioning

confidence: 99%