Sensitive detection of aflatoxin B1 molecules on gold SPR chip surface using functionalized gold nanoparticles

Abstract

Due to the warm and favourably humid climate of Southern Hungary, the maize is one of the most important crops. The protection against crop damage caused by fusarium and Aspergillus species is essential. Detection of aflatoxin B1 (AFB1) molecules in cereal crops by selective sensors is important, while they can cause serious diseases in humans and animals if they enter the food chain. Our main objective was to develop selective AFB1 sensor with increased sensitivity applying βCD-functionalized gold nanoparticles (AuβCD NPs) in surface plasmon resonance (SPR) measuring apparatus. The nanoparticles ca. 10 nm in diameter were prepared in the presence of thiol-modified cyclodextrin. The adsorption isotherms of AFB1 on bare, thiol-modified cyclodextrin and AuβCD NPs covered Au film surface were calculated using SPR platform. The AFB1 concentration can be quantitatively determined in the 0.001-23.68 ng/mL range. The AuβCD NPs were found to be highly sensitive and exhibited a remarkably low limit of detection (LOD; 1 pg/mL) without using other analytical reagents.

A label-free ultrasensitive microfluidic surface Plasmon resonance biosensor for Aflatoxin B1 detection using nanoparticles integrated gold chip

“…It has been reported that by increasing the concentration of analyte on the SPRi sensor chip surface, signals in term of response unit increases due to the binding of antigen and antibody (Park et al, 2014). Similarly, Majzik et al, also studied the interaction of AFB 1 to anti-AFB 1 biomolecules (Majzik et al, 2015). It has also been exposed that by increasing the AFB 1 concentration into the flow system, AFB 1 analyte bind with immobilized molecules over the sensor surface results to change in SPRi signal.…”

Detection of Aflatoxins in Different Matrices and Food-Chain Positions

“…Its disadvantage is that although it is sensitive, it is not selective in the case of complex samples. However, the required selectivity can be achieved by prior sample clean-up with immunoaffinity column, providing a clean solution without interferences ( Majzik et al, 2015 ).…”

Recent advances in nanomaterials integrated immunosensors for food toxin detection

“…Majzik et al demonstrated the sensitive detection method for AFB 1 molecules onto the gold SPR disk using the functionalized AuNPs (Majzik et al 2015). The thiolmodified cyclodextrin and gold b-cyclodextrin functionalized AuNPs immobilized onto the surface through covalent bonding.…”