Sensitive detection of albuminuria by graphene oxide-mediated fluorescence quenching aptasensor

Chawjiraphan, Wireeya; Apiwat, Chayachon; Segkhoonthod, Khoonsake; Treerattrakoon, Kiatnida; Pinpradup, Preedee; Sathirapongsasuti, Nuankanya; Pongprayoon, Prapasiri; Luksirikul, Patraporn; Isarankura-Na-Ayudhya, Patcharee; Japrung, Deanpen

doi:10.1016/j.saa.2020.118128

Cited by 18 publications

(30 citation statements)

References 20 publications

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“…After mixing of GHSA and the GO–aptamer complex, the fluorescence signal was recovered owing to the detachment of the aptamer from the GO surface and subsequent binding of the aptamer and GHSA, leading to the FRET reaction. The results also demonstrated that the percent fluorescence response was dependent on the concentration of GHSA ( Figure 4 b), similar to previous electrochemical results reported by Apiwat et al [ 19 ] and Chawjiraphan et al [ 24 , 25 ]. These results support the interactions of the modified G8 aptamer on the GO surface and the binding of the aptamer with GHSA in the electrochemical sensing system.…”

Section: Resultssupporting

confidence: 90%

“…To confirm the performance of the electrochemical measurement of the GO-aptasensor for GHSA detection, the modified G8 aptamer was 5′-end labeled with a fluorescence molecule (FAM), and the fluorescence intensities were measured following a modified protocol from previous reports [ 19 , 24 , 25 ]. Briefly, 2 µL of purified GHSA or clinical samples was mixed with 10 µL of GO-aptasensor complex solution and 188 µL of electrolyte solution (5 mM K 3 Fe(CN) 6 and 10 mM of PBS).…”

Section: Methodsmentioning

confidence: 99%

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Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection

et al. 2021

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An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.

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Section: Resultssupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection

et al. 2021

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“…For the corresponding articles discussed above, the limit of detection for electrophoresis, chromatography, mass spectroscopy, immunoassay, fluorescence exceeded 2 mg/L for albumin, which is enough for the clinical purpose. For some methods, explained in [ 117 , 119 , 122 , 123 , 127 , 132 , 143 ], the limit of detection does not exceed 0.1 mg/L for albumin in the urine matrix. For low abundant proteins the limit of detection must be obviously much lower.…”

Section: Discussionmentioning

confidence: 99%

“…Another interesting approach to fluorescence spectroscopy is the fluorescent quenching. Chawjiraphan et al developed a method to determine HSA in urine and serum by the graphene oxide-mediated (GO) fluorescence quenching aptasensor [ 132 ]. When albumin was added to the complex GO with the fluorescence-labeled aptamer, the aptamer detached from the complex to bind albumin, which resulted by an increase in fluorescence intensity, as shown in Figure 4 .…”

Section: Detection Techniquesmentioning

confidence: 99%

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Review: Detection and quantification of proteins in human urine

Aitekenov

Gaipov

Bukasov

2021

Talanta

125

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Extensive medical research showed that patients, with high protein concentration in urine, have various kinds of kidney diseases, referred to as proteinuria. Urinary protein biomarkers are useful for diagnosis of many health conditions – kidney and cardio vascular diseases, cancers, diabetes, infections. This review focuses on the instrumental quantification (electrophoresis, chromatography, immunoassays, mass spectrometry, fluorescence spectroscopy, the infrared spectroscopy, and Raman spectroscopy) of proteins (the most of all albumin) in human urine matrix. Different techniques provide unique information on what constituents of the urine are. Due to complex nature of urine, a separation step by electrophoresis or chromatography are often used for proteomics study of urine. Mass spectrometry is a powerful tool for the discovery and the analysis of biomarkers in urine, however, costs of the analysis are high, especially for quantitative analysis. Immunoassays, which often come with fluorescence detection, are major qualitative and quantitative tools in clinical analysis. While Infrared and Raman spectroscopies do not give extensive information about urine, they could become important tools for the routine clinical diagnostics of kidney problems, due to rapidness and low-cost. Thus, it is important to review all the applicable techniques and methods related to urine analysis. In this review, a brief overview of each technique’s principle is introduced. Where applicable, research papers about protein determination in urine are summarized with the main figures of merits, such as the limit of detection, the detectable range, recovery and accuracy, when available.

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How human serum albumin‐selective DNA aptamer binds to bovine and canine serum albumins

2021

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Serum albumin (SA) is the most abundant carrier protein in blood. SA carries a diverse range of nutrients, drugs, and metal ions. It has wide clinical and biochemical applications. Human serum albumin (HSA) can be used as a biomarker for kidney and liver diseases. Aptasensor is one of potential HSA detection methods. HSA-specific aptamer was selected for HSA detection. In animals, bovine serum albumin (BSA) and canine serum albumins (CSA) share high sequence similarities to HSA. Thus, it is interesting to explore the possibility of using HSA-selective aptamer for BSA and CSA aptasensor. In this study, molecular dynamics (MD) simulations were initially employed to investigate the binding of aptamer to BSA and CSA in comparison to HSA. Like HSA, both BSA and CSA can bind aptamer, but different binding affinities are observed. BSA shows the tighter binding to aptamer than CSA. Domain III is found to be the aptamer-binding domain although no specific aptamer conformation is captured. However, in all cases, the aptamer utilizes the 3 0-end to attach on an albumin surface. Both nucleobases and phosphate backbones on a DNA aptamer are important for albumin-aptamer complexation. Our results imply the possibility of using HSA-specific aptamer for BSA detection due to tighter binding observed, but may be less effective in CSA. However, the test in actual complicated condition must be further studied.

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Sensitive detection of albuminuria by graphene oxide-mediated fluorescence quenching aptasensor

Cited by 18 publications

References 20 publications

Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection

Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection

Review: Detection and quantification of proteins in human urine

How human serum albumin‐selective DNA aptamer binds to bovine and canine serum albumins

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