Sensitive detection of Escherichia coli O157:H7 using Pt–Au bimetal nanoparticles with peroxidase-like amplification

Lateral flow assays: Principles, designs and labels

“…Platinum (Pt) nanoparticles have peroxidase activity and are used in LFA development due to a high termostability than that of enzymes [171,173]. A lateral flow biosensor, based on PtNPs, showed consistent signal intensities at temperatures from 20 to 90 o C. However, an enzyme is denaturated in these temperatures.…”

“…Other labels used in LFA development include Fe 3 O 4 @silika nanoparticles [167,168], silika naoparticle/CdTe [169], latex beads [170], platinium nanoparticles [171], blue polysterene carboxylic acid-modified seradyn nanoparticles [172], and platinium-gold bimetal nanoparticles [173]. The developed LFAs, which are based on other materials, are summarized in Table 3.…”

“…The developed biosensor' signals amplification strategies were based on peroxidase-like activity of PtNPs toward TMB solution. TMB is a chromogenic substrate that showed the peroxidase activity in hydrogen peoxidase solution [171,173]. Park et al (2015) reported that the sensitivity of their LFA, which was based on chemiluminescence reaction, PtNPs, increased 1000 fold when compared to the conventional rapid test [171].…”

Biochars and their magnetic derivatives as enzyme-like catalysts mimicking peroxidases

“…Discovery of the peroxidase-mimetic activity of ferromagnetic nanoparticles in 2007 (Gao et al 2007) inspired the investigation of other potential peroxidase-like systems based on metallic and carbonaceous (nano)materials or their mixtures. Gold nanoparticles (Deng et al 2016), ceria nanorods (Tian et al 2015), cupric oxide nanoparticles (Chen et al 2012), Cu 2+ ions , diverse bimetallic nanoparticles (Jiang et al 2016;Zhang et al 2015), FeVO 4 nanobelts (Yu et al 2016), iron phosphate microflowers (Wang et al 2012b) and many other nanomaterials have been successfully used to catalyze peroxidase substrates oxidation in the presence of H 2 O 2 (Wei and Wang 2013).…”

“…Peroxidase-like systems can be utilized in diverse areas of bioscience, medicine and environmental technology. New types of peroxidase-mimetic (nano)particles have been successfully employed as a component of biosensors for the detection of cancer (Tian et al 2015;Zheng et al 2016), glucose (Ding et al 2016), H 2 O 2 (Qiao et al 2014), heavy metal ions , cholesterol and pathogenic bacteria (Jiang et al 2016;Park et al 2015), as well as, for degradation of xenobiotics (Guo et al 2015;Mu et al 2016;Wan et al 2016), or for inhibition of bacterial growth and biofilm elimination .…”