Sensitive Detection of &lt;I&gt;Mycoplasma pulmonis&lt;/I&gt; by Using the Polymerase Chain Reaction

Kunita, Satoshi; Terada, Eiji; Goto, Kazuo; Kagiyama, Naoko

doi:10.1538/expanim1978.39.1_103

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…More recently, PCR has been assessed as a diagnostic strategy (Brunnert et al, 1994;Goto et al, 1994;Harasawa et al, 1990;Kunita et al, 1990;Schoeb et al, 1997;van Kuppeveld et al, 1993). It appears capable of detecting infection rapidly, accurately, and sensitively in living mice or in paraffin-embedded tissues.…”

Section: Bacterial Diseasesmentioning

confidence: 99%

Biology and Diseases of Mice

Jacoby¹,

Fox²,

Davisson³

2002

Laboratory Animal Medicine

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Section: Bacterial Diseasesmentioning

confidence: 99%

Biology and Diseases of Mice

Jacoby¹,

Fox²,

Davisson³

2002

Laboratory Animal Medicine

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“…pulmonis (strain m53) [9], M. arthritidis (PG6, ATCC19611), and M. neurolyticum (type A, ATCC19988) were used to construct a database for MALDI-TOF MS. Media for cultivation of Mycoplasma strains have been described previously [4]. Briefly, an agar medium containing 70 ml of PPLO broth (BD, Tokyo, Japan) supplemented with 10 ml of 25% (w/v) yeast extract, 20 ml of heat-inactivated horse serum (Life Technologies Japan Ltd., Tokyo, Japan), 1 ml of 100,000 units of penicillin G, 1 ml of 2.5% thallium acetate, and 1.3% Bacto agar (BD) was used.…”

mentioning

confidence: 99%

Rapid Identification of <i>Mycoplasma pulmonis</i> Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Goto

Yamamoto

Asahara

et al. 2012

The Journal of Veterinary Medical Science

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“…Differentiation between mycoplasma species is necessary because M. arthritidis is naturally non-pathogenic and does not interfere with laboratory rodent research, though it is arthritogenic in mice after intravenous inoculation. Biological tests, PCR using Mycoplasma pulmonis specific primers, and Southern hybridization using a Mycoplasma pulmonis specific probe have been proposed for mycoplasma' differentiation [7,12,14].…”

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confidence: 99%

Differential Identification of Mycoplasma pulmonis and M. arthritidis Using PCR-Based RFLP

et al. 2005

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Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP).M. pulmonis is known to play a major etiological role in murine respiratory diseases in long-term rodent studies [3]. In addition, this organism produces genital infections resulting in reduced birth rates [3], and Cesarean derivation and barrier maintenance have little effect on prevention of in utero infection of M. pulmonis. A serological test is commercially used for diagnosis of M. pulmonis, however antigenic cross-reactivity between M. pulmonis and M. arthritidis interferes with interpretation of the serological test results. Accordingly, serological differentiation of M. pulmonis from M. arthritidis is needed because M. arthritidis is naturally non-pathogenic and does not interfere with laboratory rodent research, though it is arthritogenic in mice after intravenous inoculation [9]. Seoul, South Korea The objective of the present study was to develop a method of differential diagnosis of M. pulmonis and M. arthritidis.Thirty mice and rats showing dyspnea, chattering signs and seropositivity were collected from several conventional facilities. The serological test for M. pulmonis was performed using Murine Immunocomb™ (Charles River, U.S.A). The animals were euthanized by cervical dislocation, and the skin over the trachea was grasped with forceps and excised with scissors. By excising the sternohyoid muscle the trachea was exposed, washed with phosphate buffered saline (pH 7.2) and the tracheal fluid was collected. The tracheal wash was cultured on a PPLO agar plate supplemented with yeast extract (0.1 mg/ml), horse serum (20%), penicillin (3.5 mg/ml), and

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Sensitive Detection of <I>Mycoplasma pulmonis</I> by Using the Polymerase Chain Reaction

Cited by 9 publications

References 10 publications

Biology and Diseases of Mice

Biology and Diseases of Mice

Rapid Identification of <i>Mycoplasma pulmonis</i> Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Differential Identification of Mycoplasma pulmonis and M. arthritidis Using PCR-Based RFLP

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