Sensitive detection of ochratoxin A in wine and cereals using fluorescence-based immunosensing

Prieto‐Simón, Beatriz; Karube, Isao; Saiki, Hiroshi

doi:10.1016/j.foodchem.2012.05.060

Cited by 30 publications

(13 citation statements)

References 40 publications

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“…From other methods, immunochemical detection with SPR [20,21], electrochemical [21–25] and fluorescence [26–28] sensing can be mentioned (see also review [29] on immunochemical OTA detection and references cited). Direct oxidation of OTA from alkaline solution on glassy carbon electrode was evaluated by square-wave voltammetry [30].…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Evtugyn

Porfireva

Stepanova

et al. 2013

Sensors

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An impedimetric aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with a new modifier consisting of electropolymerized Neutral Red and a mixture of Au nanoparticles suspended in the dendrimeric polymer Botlorn H30®. Thiolated aptamer specific to OTA was covalently attached to Au nanoparticles via Au-S bonding. The interaction of the aptamer with OTA induced the conformational switch of the aptamer from linear to guanine quadruplex form followed by consolidation of the surface layer and an increase of the charge transfer resistance. The aptasensor makes it possible to detect from 0.1 to 100 nM of OTA (limit of detection: 0.02 nM) in the presence of at least 50 fold excess of ochratoxin B. The applicability of the aptasensor for real sample assay was confirmed by testing spiked beer samples. The recovery of 2 nM OTA was found to be 70% for light beer and 78% for dark beer.

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Section: Introductionmentioning

confidence: 99%

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Evtugyn

Porfireva

Stepanova

et al. 2013

Sensors

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“…Nanomaterials play an important role with respect to the enhancement of the sensitivity in the fluorescence recovery strategy. Colloidal gold nanoparticles [22], dendrimers [19], quantum dots [23], and graphene oxide [24] have been employed in the mycotoxin sensing [25]. In combination with nanoparticles, specific aptamers were also used to develop fluorescence sensors for mycotoxin detection [26,27].…”

Section: Sensing Strategiesmentioning

confidence: 99%

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Zhang

et al. 2016

Toxins

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Mycotoxin contamination threatens health and life of humans and animals throughout the food supply chains. Many of the mycotoxins have been proven to be carcinogens, teratogens and mutagens. The reliable and sensitive sensing methods are requested to monitor mycotoxin contamination. Advanced sensors based on antibodies or aptamers boast the advantages of high sensitivity and rapidity, and have been used in the mycotoxin sensing. These sensors are miniaturized, thereby lowering costs, and are applicable to high-throughput modes. In this work, the latest developments in sensing strategies for mycotoxin determination were critically discussed. Optical and electrochemical sensing modes were compared. The sensing methods for single mycotoxin or multiple mycotoxins in food samples were reviewed, along with the challenges and the future of antibody or aptamer-based sensors. This work might promote academic studies and industrial applications for mycotoxin sensing.

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“…The toxicological profile of OTA includes teratogenesis, nephrotoxicity and immunotoxicity, and it has been classified by the International Agency for Research on Cancer as a possible human carcinogen (group 2B). In order to protect consumers from risk related to OTA, the European Commission has set a maximum limit of 5 µg kg −1 for OTA in raw wheat, barley and rye …”

Section: Introductionmentioning

confidence: 99%

“…In order to protect consumers from risk related to OTA, the European Commission has set a maximum limit of 5 μg kg −1 for OTA in raw wheat, barley and rye. 2,3 In recent years a variety of well-established methodologies have been developed for rapid quantitative or semi-quantitative analysis of OTA in foods and beverages, such as thin layer chromatography, 4 high-performance liquid chromatography with fluorescent detection, 5 gas chromatography coupled with mass spectrometry 6 and enzyme-linked immunosorbent assay (ELISA). 7 However, chromatographic methods are expensive, laborious and time-consuming and need skilled personnel, so a rapid, cost-effective, field-portable assay system is needed for rapid OTA screening of contaminated samples.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay

et al. 2015

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Sensitive detection of ochratoxin A in wine and cereals using fluorescence-based immunosensing

Cited by 30 publications

References 40 publications

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay

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