Sensitive detection of the okadaic acid marine toxin in shellfish by Au@Pt NPs/horseradish peroxidase dual catalysis immunoassay

Tian, Yinqi; Yuan, Lin; Zhang, Min; He, Youfen; Lin, Xucong

doi:10.1039/d1ay01973b

Cited by 13 publications

(10 citation statements)

References 40 publications

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“…In general, LODs of OA are lower in ELISAs, approximately reaching picogram values [ 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. In ICAs [ 44 , 45 , 46 , 47 , 48 , 49 ], including multiparametric ones [ 18 , 20 , 50 ], the sensitivity of the determination is somewhat worse (up to the nanogram concentration range), and improvements in this method were achieved with amplification approaches aimed at increasing analytical signal [ 19 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

A Sensitive Fluorescence Polarization Immunoassay for the Rapid Detection of Okadaic Acid in Environmental Waters

et al. 2023

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In this study, a homogeneous fluorescence polarization immunoassay (FPIA) for the detection of hazardous aquatic toxin okadaic acid (OA) contaminating environmental waters was for the first time developed. A conjugate of the analyte with a fluorophore based on a fluorescein derivative (tracer) was synthesized, and its interaction with specific anti-OA monoclonal antibodies (MAbs) was tested. A MAbs–tracer pair demonstrated highly affine immune binding (KD = 0.8 nM). Under optimal conditions, the limit of OA detection in the FPIA was 0.08 ng/mL (0.1 nM), and the working range of detectable concentrations was 0.4–72.5 ng/mL (0.5–90 nM). The developed FPIA was approbated for the determination of OA in real matrices: river water and seawater samples. No matrix effect of water was observed; therefore, no sample preparation was required before analysis. Due to this factor, the entire analytical procedure took less than 10 min. Using a compact portable fluorescence polarization analyzer enables the on-site testing of water samples. The developed analysis is very fast, easy to operate, and sensitive and can be extended to the determination of other aquatic toxins or low-molecular-weight water or food contaminants.

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Section: Resultsmentioning

confidence: 99%

A Sensitive Fluorescence Polarization Immunoassay for the Rapid Detection of Okadaic Acid in Environmental Waters

et al. 2023

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“…In that work, another kind of immunoassay, namely ELISA, was implemented, which needed laboratory equipment for its completion. Tian et al (2021) developed a competitive indirect ELISA of OA in shellfish with the Au@Pt NPs/horseradish peroxidase label [ 38 ]. ELISA typically has lower LODs than LFIA, and applying the nanozyme led to a further increase in sensitivity; the LOD of OA was 40 pg/mL.…”

Section: Resultsmentioning

confidence: 99%

Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid

et al. 2022

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In this study, a lateral flow immunoassay (LFIA) was developed to detect okadaic acid (OA) belonging to the diarrheic shellfish poisoning group of aquatic toxins. Newly obtained anti-OA monoclonal antibodies and bimetallic core@shell Au@Pt nanoparticles were used in the indirect format of the LFIA. Peroxidase-mimicking nanozyme properties of Au@Pt enabled using them to enhance band coloration on the test strips and, consequently, for increasing the LFIA sensitivity. The instrumental limit of detection (LOD), the working range of detectable concentrations, and the visual cutoff of the assay were 0.5, 0.8–6.8, and 10 ng/mL, respectively. The assay duration was 20 min. The rapid and simple sample preparation procedure was applied for seawater, river water, and fish samples. The total duration of the sample pretreatment and LFIA was 25/40 min for water/fish samples, ensuring testing rapidity. The developed test system provides sensitive control of raw materials and food products and can be used to detect OA at all stages of the food industry «from sea to fork» chains.

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“…The LFIA of OA has been described in several studies [ 21 , 22 , 23 , 24 , 25 ]. Most of these works are based on the routine direct competitive LFIA with AuNPs as a label for specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

“…The reported test systems enable the determination of OA with LODs varying in the range of 0.1–50 ng/mL. Only one recent study is devoted to the development of an enhancement strategy based on the catalysis of Au@Pt nanoparticles and horseradish peroxidase [ 25 ]. With this approach, the authors achieved an OA LOD of 0.04 ng/mL.…”

Section: Introductionmentioning

confidence: 99%

Cascade-Enhanced Lateral Flow Immunoassay for Sensitive Detection of Okadaic Acid in Seawater, Fish, and Seafood

Hendrickson

Zvereva

Zherdev

et al. 2022

Foods

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In this investigation, a new approach for developing a sensitive lateral flow immunoassay (LFIA) was proposed for the detection of the hazardous marine toxin okadaic acid (OA). It is based on the indirect format with anti-species antibodies labeled by gold nanoparticles (AuNPs) and cascade signal amplification. The latter is performed by first passing a mixture of anti-OA antibodies and a tested sample along the immunochromatographic test strip and then performing several cycles of the interaction of anti-species antibodies conjugated with AuNPs with free antibodies, which bind to anti-species antibodies but are not specific to the target analyte. As a result, branched aggregates are formed, due to which the colorimetric signal intensification occurs. The developed test system enabled the detection of OA with an instrumental detection limit of 30 pg/mL and a cutoff of 1 ng/mL, which exceeds these characteristics in the LFIA without amplification by 7 and 2 times, respectively. The OA recoveries from seawater, fish, and seafood varied from 76.9% to 126%. The test system may be required for point-of-care monitoring of samples for phycotoxin contamination; the developed principle of signal amplification can be used in cases where highly sensitive detection of trace amounts of a contaminant is required.

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Sensitive detection of the okadaic acid marine toxin in shellfish by Au@Pt NPs/horseradish peroxidase dual catalysis immunoassay

Abstract: Based on the catalysis enhanced strategy of Au@Pt nanoparticles (Au@Pt NPs) and horseradish peroxidase (HRP) related to TMB-H2O2 indicator, a sensitive colorimetric immunoassay was established for trace okadaic acid (OA)...

Cited by 13 publications

References 40 publications

A Sensitive Fluorescence Polarization Immunoassay for the Rapid Detection of Okadaic Acid in Environmental Waters

A Sensitive Fluorescence Polarization Immunoassay for the Rapid Detection of Okadaic Acid in Environmental Waters

Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid

Cascade-Enhanced Lateral Flow Immunoassay for Sensitive Detection of Okadaic Acid in Seawater, Fish, and Seafood

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