Sensitive determination of neurotransmitters in urine by microchip electrophoresis with multiple-concentration approaches combining field-amplified and reversed-field stacking

Zhang, Yan; Zhang, Yi; Wang, Guan; Chen, Wujuan; Yi, Li; Zhang, Yating; He, Pingang; Wang, Qingjiang

doi:10.1016/j.jchromb.2016.04.054

Cited by 20 publications

(8 citation statements)

References 29 publications

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“…Zhang et al. presented a method for the determination of neurotransmitters in urine by microchip electrophoresis with multiple‐concentration approaches combining field‐amplified and reversed‐field stacking with LIF detection. Using 90 mM sodium borate at pH 9.4 as the BGE, separation of three neurotransmitters (dopamine, norepinephrine and serotonin) was achieved with LODs of 1.69, 2.35, and 2.73 nM, respectively.…”

Section: Concentration Adjustment (Kohlrausch Stacking)mentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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Electrophoretic sample stacking comprises a group of capillary electrophoretic techniques where trace analytes from the sample are concentrated into a short zone (stack). This paper is a continuation of our previous reviews on the topic and brings a survey of more than 120 papers published approximately since the second quarter of 2016 till the first quarter of 2018. It is organized according to the particular stacking principles and includes chapters on concentration adjustment (Kohlrausch) stacking, on stacking techniques based on pH changes, on stacking in electrokinetic chromatography and on other stacking techniques. Where available, explicit information is given about the procedure, electrolyte(s) used, detector employed and sensitivity reached. Not reviewed are papers on transient isotachophoresis which are covered by another review in this issue.

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Section: Concentration Adjustment (Kohlrausch Stacking)mentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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“…An example of this type of analysis is given by Vlčková and Schwarz who developed an ME-based method for simultaneous determination of catecholamines (DA, adrenaline, and noradrenaline (NE)) and their O-methoxylated metabolites (methoxytyramine, normetanephrine, and metanephrine) in mouse brain homogenates [129]. A simple and rapid method employing an on-line multiple-preconcentration approach combining field-amplified stacking and reversed-field stacking for the simultaneous analysis of 5-HT, NE, and DA neurotransmitters in urine samples by ME-LIF was developed by Zhang et al [130]. Their optimized system allowed a very quick (3 min) neurotransmitters separation characterized by nanomolar-level LODs (1.69 for 5-HT, 2.35 for NE, and 2.73 for DA).…”

Section: Me For Separation and Quantification Of Neurotransmitters Inmentioning

confidence: 99%

Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis

et al. 2020

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The last decades of biological, toxicological, and pharmacological research have deeply changed the way researchers select the most appropriate ‘pre-clinical model’. The absence of relevant animal models for many human diseases, as well as the inaccurate prognosis coming from ‘conventional’ pre-clinical models, are among the major reasons of the failures observed in clinical trials. This evidence has pushed several research groups to move more often from a classic cellular or animal modeling approach to an alternative and broader vision that includes the involvement of microfluidic-based technologies. The use of microfluidic devices offers several benefits including fast analysis times, high sensitivity and reproducibility, the ability to quantitate multiple chemical species, and the simulation of cellular response mimicking the closest human in vivo milieu. Therefore, they represent a useful way to study drug–organ interactions and related safety and toxicity, and to model organ development and various pathologies ‘in a dish’. The present review will address the applicability of microfluidic-based technologies in different systems (2D and 3D). We will focus our attention on applications of microchip electrophoresis (ME) to biological and toxicological studies as well as in drug discovery and development processes. These include high-throughput single-cell gene expression profiling, simultaneous determination of antioxidants and reactive oxygen and nitrogen species, DNA analysis, and sensitive determination of neurotransmitters in biological fluids. We will discuss new data obtained by ME coupled to laser-induced fluorescence (ME-LIF) and electrochemical detection (ME-EC) regarding the production and degradation of nitric oxide, a fundamental signaling molecule regulating virtually every critical cellular function. Finally, the integration of microfluidics with recent innovative technologies—such as organoids, organ-on-chip, and 3D printing—for the design of new in vitro experimental devices will be presented with a specific attention to drug development applications. This ‘composite’ review highlights the potential impact of 2D and 3D microfluidic systems as a fast, inexpensive, and highly sensitive tool for high-throughput drug screening and preclinical toxicological studies.

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“…Recently, microchip electrophoresis (MCE), conserving the advantages of low sample consumption and high analysis speed, is increasingly being viewed as a successful alternative to CE technology for rapid analysis. Therefore, an on-line preconcentration strategy combining FASS and reversed-field stacking (RFS) was developed for the efficient and sensitive analysis of DA, NA and 5-HT in real urine samples by MCE with laser-induced fluorescence (LIF) detection [99]. The proposed multiple-preconcentration strategy greatly improved the sensitivity enhancement and surpassed other conventional analytical methods for the quantification of neurotransmitters and can yield a 182- to 292-fold increase in detection sensitivity (Figure 11).…”

Section: Analytical Strategies Of Ba Determinations Based On Separmentioning

confidence: 99%

“…Peak identification: 1, the excess of Cy5; 2, 5-HT; 3, DA; and 4, NE. Figure adopted from reference [99] with the copyright permission.…”

Section: Figurementioning

confidence: 99%

Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

Plenis

Olędzka

Kowalski

et al. 2019

JCM

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Biogenic amines (BAs) are bioactive endogenous compounds which play a significant physiological role in many cell processes like cell proliferation and differentiation, signal transduction and membrane stability. Likewise, they are important in the regulation of body temperature, the increase/decrease of blood pressure or intake of nutrition, as well as in the synthesis of nucleic acids and proteins, hormones and alkaloids. Additionally, it was confirmed that these compounds can be considered as useful biomarkers for the diagnosis, therapy and prognosis of several neuroendocrine and cardiovascular disorders, including neuroendocrine tumours (NET), schizophrenia and Parkinson’s Disease. Due to the fact that BAs are chemically unstable, light-sensitive and possess a high tendency for spontaneous oxidation and decomposition at high pH values, their determination is a real challenge. Moreover, their concentrations in biological matrices are extremely low. These issues make the measurement of BA levels in biological matrices problematic and the application of reliable bioanalytical methods for the extraction and determination of these molecules is needed. This article presents an overview of the most recent trends in the quantification of BAs in human samples with a special focus on liquid chromatography (LC), gas chromatography (GC) and capillary electrophoresis (CE) techniques. Thus, new approaches and technical possibilities applied in these methodologies for the assessment of BA profiles in human samples and the priorities for future research are reported and critically discussed. Moreover, the most important applications of LC, GC and CE in pharmacology, psychology, oncology and clinical endocrinology in the area of the analysis of BAs for the diagnosis, follow-up and monitoring of the therapy of various health disorders are presented and critically evaluated.

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Sensitive determination of neurotransmitters in urine by microchip electrophoresis with multiple-concentration approaches combining field-amplified and reversed-field stacking

Cited by 20 publications

References 29 publications

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis

Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

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