Sensitive Determination of Prulifloxacin by Its Fluorescence Enhancement on Terbium(III)–Sodium Dodecylbenzene Sulfonate System

Abstract

A terbium-sensitized fluorescence spectrophotometry method using an anionic surfactant, sodium dodecyl benzene sulphonate (SDBS), was developed for the determination of prulifloxacin (PUFX). It was found that SDBS significantly enhanced the fluorescence intensity of the PUFX-Tb 3þ complex (about 13-fold). The optimal experimental conditions were determined as follows: excitation and emission wavelengths of 290 nm and 545 nm, pH 8.0, 4.0 Â 10 À5 mol L À1 terbium(III), and 4.0 Â 10 À4 mol L À1 SDBS. The enhanced fluorescence intensity of the system (DF) showed a good linear relationship with the concentration of PUFX over the range 6.0 Â 10 À8 to 2.0 Â 10 À6 mol L À1 with a correlation 3124 coefficient of 0.9991. The detection limit (S=N ¼ 3) was determined as 8.5 Â 10 À9 mol L À1 . This method has been successfully applied for the determination of PUFX in pharmaceuticals and human urine=serum samples. Compared with most other methods reported, the rapid and simple procedure proposed here offered higher sensitivity, wider linear range, and good stability. The luminescence mechanism of the system was also discussed in detail. In the fluorescence system of PUFX-Tb 3þ -SDBS, SDBS acted not only as the surfactant but also as the energy donor.

Stability-indicating micellar enhanced spectro-fluorometric determination of Daclatasvir in its tablet and spiked human plasma

“…A likely contributing factor here is the protection of the lowest excited singlet state in the micellar micro-environment from non-radiative processes [18]. In addition, sequestering of the drug into SDS can potentially cause a decrease in non-radiative energy loss via molecular collisions resulting in an enhancement of the fluorescence quantum efficiency [27]. The optimum concentration of SDS is 6.90 mM which is above the critical micelle concentration value (6 mM) showing that the presence of micelles significantly enhances the DAC fluorescence intensity [25].…”

“…Therefore, 6.90 mM SDS solution was chosen as the optimum SDS concentration to achieve maximum enhancement in the FI of DAC. This is related to the observation that the DAC FI increases with increasing SDS concentration and reaches a maximum close to the critical micelle concentration of SDS (6 mM)[25,26], which is advantageous for increasing the effective absorption cross section of the complex causing increase of molar absorbancy index[27].…”

Stability study of Prulifloxacin and Ulifloxacin in human plasma by HPLC–DAD

“…(a) methanol-water (both containing 0.5% formic acid, v/v) 13 , (b) acetonitrile-0.5% triethylamine buffer (pH 3.0) 14 and (c) ammonium acetate buffer (5 mM, pH 5.8) and acetonitrile (12:88, v/v), respectively.…”

“…Several papers report high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of Ulifloxacin in plasma 12,13 . Some published works also report an HPLC method with fluorescence detection (FLD) 14 . The assay employed solid phase extraction (SPE) involving multi-step purification and nitrogen evaporation and 0.5 mL plasma was needed.…”

Gold nanoparticles‐based fluorescence enhancement of the terbium–levofloxacin system and its application in pharmaceutical preparations

“…A large number of spectrofluorimetry methods have been used for the assay of drugs and metals and for the determination of low concentrations of other analytes (11)(12)(13). The terbium-sensitized fluorescence method has been used in the determination of fluoroquinolones (14)(15)(16).…”